Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
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muscle
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• anterograde shifts of the cranial and caudal borders of the multifidus muscles in 13 of 15 mice
• in 6 of 15 mice there is a unilateral or bilateral anterograde shift of the cranial multifidus border to C2 or C1 rather than T2
• results in the addition of new muscle segments with novel insertion sites craniad to T2
• posterograde shifts of the cranial multifidus border are also seen with cranial insertion points at only T6 or T9 rather than T2
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skeleton
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• fusions and bifurcations resulting in highly variable intercostal distances
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• widespread vertebral defects at almost all vertebral levels
• defects are more severe in the lumbar region than in the thoracic and cervical regions
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• fusions are seen
• however, the spinous process at T2 is present and distinct
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Allelic
Composition |
Lfngtm1Rjo/Lfngtm1Rjo
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Genetic
Background |
either: (involves: 129S7/SvEvBrd * C57BL/6J) or (involves: 129S7/SvEvBrd * C57BL/6J * FVB/N) |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
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mortality/aging
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• survival of homozygotes postnatally is only rarely observed
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embryo
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• at E9.5, little compartmentalization of somites is observed, with rostral and caudal cells appearing mixed
• at E10.5, somites in thoracic region fail to compartmentalize, maintaining an unsegmented pattern and this continues during secondary body formation
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skeleton
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• in thoracic region, multiple rib fusions are observed
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• thoracic vertebrae are disorganized; vertebral condensations are irregular and misaligned
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• vertebral disorganization extends through lumbar region; vertebral condensations are irregular and misaligned
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• vertebral disorganization extends through sacral region; vertebral condensations are irregular and misaligned
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limbs/digits/tail
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• tail is severely truncated
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reproductive system
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• males surviving to adulthood are sterile
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Allelic
Composition |
Lfngtm1Rjo/Lfngtm1Rjo
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Genetic
Background |
involves: 129P2/OlaHsd * 129S7/SvEvBrd * C57BL/6J * FVB/NJ |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
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mortality/aging
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• fewer than expected are found at weaning however survivors are viable and fertile
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• fewer than expected are found at weaning
• Background Sensitivity: survival is improved compared to mice on a mixed C57BL/6J and 129 background
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skeleton
limbs/digits/tail
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
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Regional and dose-dependent rescue of the Lfng phenotype by transgenic expression in the stripe domain
mortality/aging
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• relative viability compared to wild-type controls is 9%
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• most die before weaning
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skeleton
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• have either 1 or 0 tail vertebrae
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• have either 1 or 0 tail vertebrae
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• at E10.5 - E11.5, sclerotomes cranial to the hindlimb buds are fused and borders never become as visible as in wild-type controls
• at E11.5 the PAX1 domain in the tail is thicker indicating limited formation of the scleretome
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embryo
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• embryos are rostralized,compared to wild-type
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• axial extension ceases around E10.5 resulting in a truncated tail
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• somites become increasingly irregular towards the end of the tail
• however, segmentation in the sacrum and adjacent tail area is regular
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limbs/digits/tail
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• have either 1 or 0 tail vertebrae
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• have either 1 or 0 tail vertebrae
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
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mortality/aging
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• homozygotes die late in embryonic lethality
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skeleton
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• incompletely formed vertebrae
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embryo
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• failure of caudal and rostral spatial definition of half-somites
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• when mutant fetal liver cells is mixed 1:1 with wild-type bone marrow and transferred into lethally irradiated hosts, there is a slight reduction in the number of marginal zone B cells generated compared to controls
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reproductive system
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• reproductive tracts of 4 and 7 week old null mice are smaller than heterozygous littermates
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• sexually mature mice had normal follicles of all sizes and abnormal follicles
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• ovaries of 4 and 7 week old null mice are smaller than heterozugous littermates
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• after induced ovulation and fertilization, only 9.7% of eggs became 2-cell embryos compared to 48.8% of controls
• only 2% of embryos became 4 to 8 cell embryos and none reached the blastocyst stage compared to 31.4% of controls that reached blastocysts stage
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• oocytes do not complete meiotic maturation
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• all females found infertile due to defects in meiotic maturation
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endocrine/exocrine glands
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• sexually mature mice had normal follicles of all sizes and abnormal follicles
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• ovaries of 4 and 7 week old null mice are smaller than heterozugous littermates
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hematopoietic system
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• when mutant fetal liver cells is mixed 1:1 with wild-type bone marrow and transferred into lethally irradiated hosts, there is a slight reduction in the number of marginal zone B cells generated compared to controls
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cellular
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• after induced ovulation and fertilization, only 9.7% of eggs became 2-cell embryos compared to 48.8% of controls
• only 2% of embryos became 4 to 8 cell embryos and none reached the blastocyst stage compared to 31.4% of controls that reached blastocysts stage
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
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mortality/aging
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• mice rarely survive postnatally
• Background Sensitivity: survival is reduced compared to mice on a mixed C57BL/6, 129, and FVB/NJ background
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
Mfngtm1Cjg mutation
(0 available);
any
Mfng mutation
(33 available)
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embryo
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• when mutant fetal liver cells is transferred into lethally irradiated hosts, there is a 50% reduction in the number of marginal zone B cells generated compared to controls
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hematopoietic system
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• when mutant fetal liver cells is transferred into lethally irradiated hosts, there is a 50% reduction in the number of marginal zone B cells generated compared to controls
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
Mfngtm1Seco mutation
(1 available);
any
Mfng mutation
(33 available)
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mortality/aging
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• fewer than expected are found at weaning however survivors are viable and fertile
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• fewer than expected are found at weaning
• reduction in numbers found at weaning is not greater than that of Lfngtm1Rjo single homozygous littermates
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skeleton
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• skeleton phenotype is not significantly different from that of Lfngtm1Rjo single homozygous mice
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limbs/digits/tail
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
Mfngtm1Seco mutation
(1 available);
any
Mfng mutation
(33 available)
Rfngtm1Tfv mutation
(0 available);
any
Rfng mutation
(15 available)
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mortality/aging
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• fewer than expected are found at weaning however survivors are viable and fertile
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• fewer than expected are found at weaning
• reduction in numbers found at weaning is not greater than that of Lfngtm1Rjo single homozygous littermates
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skeleton
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• skeleton phenotype is not significantly different from that of Lfngtm1Rjo single homozygous mice
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limbs/digits/tail
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N |
• unlike in chick embryo experiments, no limb abnormalities are seen
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
Mesp2tm15.1(Lfng)Ysa mutation
(0 available);
any
Mesp2 mutation
(30 available)
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embryo
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• the segmental morphology in the developing embryos is similar to that in Lfngtm1Rjo single homozygotes
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• abnormal vertebral morphology along the anteroposterior axis as is seen in Lfngtm1Rjo single homozygotes
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
Tg(Hes7-Lfng,-EGFP)#Ysa mutation
(0 available)
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embryo
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N |
• no defects in somite segmentation are detected unlike in null mice not carrying the transgene
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skeleton
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N |
• no defects are detected in the skeletal system unlike in null mice not carrying the transgene
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
Ripply2tm1.1Ysa mutation
(1 available);
any
Ripply2 mutation
(15 available)
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embryo
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• embryos show a more rostralized phenotype than Ripply2-null embryos
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skeleton
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• pedicals of the neural arches are almost completely absent
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
Tg(Lfng-LFNG)1Dihz mutation
(0 available)
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Regional and dose-dependent rescue of the Lfng phenotype by transgenic expression in the stripe domain
mortality/aging
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• survival is improved compared to null mice not carrying the transgene but still less than that of wild-type controls
• relative viability compared to wild-type controls is 33%
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skeleton
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• many mice have extra pair of true ribs
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• many mice have extra rib-bearing thoracic vertebrae
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• many mice are missing a lumbar vertebra
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• many mice have extra rib-bearing thoracic vertebra indicating homeotic transformation
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reproductive system
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
Tg(Lfng-LFNG)2Dihz mutation
(0 available)
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Adult tail phenotype of Lfngtm1Rjo/Lfngtm1Rjo and Hes7tm1.1Dihz/Hes7tm1.1Dihz mice and the effect on the Lfng tail phenotype by transgenic expression in the stripe domain
mortality/aging
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• survival is improved compared to null mice not carrying the transgene but still less than that of wild-type controls
• relative viability compared to wild-type controls is 68%
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skeleton
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• many mice have extra pair of true ribs
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• many mice have extra rib-bearing thoracic vertebrae
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• many mice are missing a lumbar vertebra
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• many mice have extra rib-bearing thoracic vertebra indicating homeotic transformation
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reproductive system
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
Tg(Lfng*-LFNG)2Dihz mutation
(0 available)
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Regional and dose-dependent rescue of the Lfng phenotype by transgenic expression in the stripe domain
mortality/aging
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• relative viability compared to wild-type controls is 9%
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skeleton
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• severely disorganized and truncated axial skeleton with irregular and fused vertebrae and vertebral bodies that rarely align with the midline
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• slight restoration of thoracic vertebrae morphology compared to null mice not carrying the transgene
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• slight restoration of cervical vertebrae morphology compared to null mice not carrying the transgene
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• slight restoration of lumbar vertebrae morphology compared to null mice not carrying the transgene
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• truncated axial skeleton
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limbs/digits/tail
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• tails are longer than in null mice not carrying the transgene but still shorter than in wild-type controls
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
Tg(Lfng*-LFNG)1Dihz mutation
(0 available)
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Regional and dose-dependent rescue of the Lfng phenotype by transgenic expression in the stripe domain
mortality/aging
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• survival is improved compared to null mice not carrying the transgene but still less than that of wild-type controls
• relative viability compared to wild-type controls is 24%
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skeleton
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• severely disorganized and truncated axial skeleton with irregular and fused vertebrae and vertebral bodies that rarely align with the midline
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• slight restoration of thoracic vertebrae morphology compared to null mice not carrying the transgene
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• slight restoration of cervical vertebrae morphology compared to null mice not carrying the transgene
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• slight restoration of lumbar vertebrae morphology compared to null mice not carrying the transgene
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• truncated axial skeleton
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limbs/digits/tail
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• tails are longer than in null mice not carrying the transgene but still shorter than in wild-type controls
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lfngtm1Rjo mutation
(0 available);
any
Lfng mutation
(32 available)
Tg(Lfng*-LFNG)1Dihz mutation
(0 available)
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Regional and dose-dependent rescue of the Lfng phenotype by transgenic expression in the stripe domain
mortality/aging
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• survival is improved compared to null mice not carrying the transgene but still less than that of wild-type controls
• relative viability compared to wild-type controls is 20%
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skeleton
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• severely disorganized and truncated axial skeleton with irregular and fused vertebrae and vertebral bodies that rarely align with the midline
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• rescue of abnormalities is confined to segmentation of the tail area
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• truncated axial skeleton
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limbs/digits/tail
Analysis Tools