Phenotypes associated with this allele

• Allele Symbol: Vegfa⁺
• Allele Name: wild type
• Allele ID: MGI:1889790
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Vegfa^tm1Dco/Vegfa^+	involves: 129	MGI:3849561
ht2	Vegfa^tm2.1Nagy/Vegfa^+	involves: 129S1/Sv * 129X1/SvJ	MGI:4422208
ht3	Vegfa^tm1Gne/Vegfa^+	involves: 129S2/SvPas * C57BL/6J	MGI:2174800
cn4	Vegfa^tm2Gne/Vegfa^+ Tg(Nphs1-cre)1Seq/0	involves: 129	MGI:2654003
cn5	Vegfa^tm2Gne/Vegfa^+ Tg(SFTPC-rtTA)5Jaw/0 Tg(tetO-cre)1Jaw/0	involves: 129 * C57BL/6	MGI:4938194
cn6	Vegfa^tm2Gne/Vegfa^+ Tg(Nes-cre)1Kln/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL	MGI:4422199

Genotype
MGI:3849561

ht1

• Allelic Composition: Vegfa^tm1Dco/Vegfa⁺
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

abnormal dorsal aorta morphology ( J:32219 )

• the dorsal aorta has a smaller lumen and fewer endothelial cells than in wild-type mice

• at E9.5, the anterior part of the dorsal aorta is poorly developed

decreased angiogenesis ( J:32219 )

• the density of sprouting intersomitic and head mesenchyme vessels is decreased compared to in wild-type mice

abnormal vitelline vasculature morphology ( J:32219 )

• however, endothelial cell density and proliferation rates are normal

• at E9.5, yolk sacs display an irregular plexus of small vessels with no larger collecting vessels unlike in wild-type mice

embryo

abnormal vitelline vasculature morphology ( J:32219 )

• at E9.5, yolk sacs display an irregular plexus of small vessels with no larger collecting vessels unlike in wild-type mice

• however, endothelial cell density and proliferation rates are normal

Genotype
MGI:4422208

ht2

• Allelic Composition: Vegfa^tm2.1Nagy/Vegfa⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

cardiovascular system

abnormal brain vasculature morphology ( J:86207 )

• at P1, mice exhibit a decrease in the number of branch points of blood vessels in the forebrain and cortex while the distance between branch points is increased compared with wild-type mice

abnormal vascular branching morphogenesis ( J:86207 )

• at P1, mice exhibit a decrease in the number of branch points of blood vessels in the forebrain and cortex while the distance between branch points is increased compared with wild-type mice

nervous system

abnormal brain vasculature morphology ( J:86207 )

• at P1, mice exhibit a decrease in the number of branch points of blood vessels in the forebrain and cortex while the distance between branch points is increased compared with wild-type mice

Genotype
MGI:2174800

ht3

• Allelic Composition: Vegfa^tm1Gne/Vegfa⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:32218 )

• heterozygotes die between E11 and E12

embryo

abnormal placenta vasculature ( J:32218 )

• at E10.5, mutant placentas exhibit a condensed vasculature with a degenerating endothelium lining the fetal capillaries

abnormal vitelline vasculature morphology ( J:32218 )

• at E9.5-E11.5, several heterozygotes display an apparent absence of blood supply in the yolk sac

abnormal vitelline vein morphology ( J:32218 )

• at E9.5-E11.5, vitelline veins fail to fuse with the yolk sac

increased embryonic tissue cell apoptosis ( J:32218 )

• at E11.5, heterozygus mutant embryos exhibit significant apoptosis, consistent with a vascular deficit

abnormal pharyngeal arch morphology ( J:32218 )

• at E9.5-E12.5, heterozygotes display poorly developed and unsegmented branchial arches in the cranial region

abnormal forelimb bud morphology ( J:32218 )

• at E9.5-E11.5, the forelimb buds of heterozygotes are abnormally located at the earlier caudal position and appear unsegmented

abnormal visceral yolk sac blood island morphology ( J:32218 )

• at E9.5-E11.5, heterozygotes show significantly less nucleated red blood cells in the blood islands

cardiovascular system

abnormal blood vessel morphology ( J:32218 )

• at E10.5, heterozygotes display abnormal vascular patterns in the placenta and forebrain

abnormal dorsal aorta morphology ( J:32218 )

• at E9.5-E11.5, heterozygotes exhibit a rudimentary dorsal aorta

abnormal placenta vasculature ( J:32218 )

• at E10.5, mutant placentas exhibit a condensed vasculature with a degenerating endothelium lining the fetal capillaries

abnormal vitelline vasculature morphology ( J:32218 )

• at E9.5-E11.5, several heterozygotes display an apparent absence of blood supply in the yolk sac

abnormal vitelline vein morphology ( J:32218 )

• at E9.5-E11.5, vitelline veins fail to fuse with the yolk sac

delayed heart development ( J:32218 )

• at E9.5-E11.5, the common atrium and primitive ventricle are developmentally delayed

• at E10.5, heterozygotes show lack of myoblast differentiation in the cardiac region

thin ventricular wall ( J:32218 )

• at E9.5-E11.5, heterozygotes show a significantly thinned ventricular wall

nervous system

increased embryonic neuroepithelium apoptosis ( J:32218 )

• at E10.5, heterozygotes exhibit increased apoptosis and disorganization of neuroepithelial cells

abnormal forebrain development ( J:32218 )

• at E9.5-E11.5, heterozygotes display an underdeveloped forebrain region with vascular elements present in the mesenchyme but not in the neuroepithelium

limbs/digits/tail

abnormal forelimb bud morphology ( J:32218 )

• at E9.5-E11.5, the forelimb buds of heterozygotes are abnormally located at the earlier caudal position and appear unsegmented

craniofacial

abnormal pharyngeal arch morphology ( J:32218 )

• at E9.5-E12.5, heterozygotes display poorly developed and unsegmented branchial arches in the cranial region

cellular

increased embryonic tissue cell apoptosis ( J:32218 )

• at E11.5, heterozygus mutant embryos exhibit significant apoptosis, consistent with a vascular deficit

increased embryonic neuroepithelium apoptosis ( J:32218 )

• at E10.5, heterozygotes exhibit increased apoptosis and disorganization of neuroepithelial cells

Genotype
MGI:2654003

cn4

• Allelic Composition: Vegfa^tm2Gne/Vegfa⁺; Tg(Nphs1-cre)1Seq/0
• Genetic Background: involves: 129

behavior/neurological

lethargy ( J:82440 )

• mutants are lethargic at 9-12 weeks of age

hematopoietic system

anemia ( J:82440 )

• normochromic, normocytic anemia

homeostasis/metabolism

increased circulating creatinine level ( J:82440 )

• more than 10 times the nomral levels of creatinine

increased blood urea nitrogen level ( J:82440 )

• elevation in urea

increased urine protein level ( J:82440 )

albuminuria ( J:82440 )

renal/urinary system

increased urine protein level ( J:82440 )

albuminuria ( J:82440 )

abnormal kidney morphology ( J:82440 )

• renal lesions are first detected at 2.5 weeks of age with swelling of the endothelial cells and hyaline deposits

abnormal glomerular capillary morphology ( J:82440 )

• no patent capillary loops can be seen at 9 weeks of age

abnormal glomerular capillary endothelium morphology ( J:82440 )

• at 2.5 weeks of age, mice display swelling of the endothelial cells (endotheliosis)

• endothelial cells become necrotic by 9 weeks of age

absent glomerular endothelium fenestra ( J:82440 )

• endothelial fenestrations are no longer identifiable by 9 weeks of age

• however, well-formed endothelial fenestrations are present at 2.5 weeks of age

abnormal podocyte morphology ( J:82440 )

• podocytes containing large empty cytoplasmic vacuoles are seen by 9 weeks of age

podocyte foot process effacement ( J:82440 )

• no podocyte foot processes are identifiable by 9 weeks of age

increased renal glomerulus basement membrane thickness ( J:82440 )

• at 6.5 weeks of age, the glomerular basement membrane is expanded

expanded mesangial matrix ( J:82440 )

• expansion of the mesangial matrix is seen by 9 weeks of age

glomerulosclerosis ( J:82440 )

• hyaline deposits are first detected at 2.5 weeks of age

kidney atrophy ( J:82440 )

• pale, shrunken kidneys at 9 weeks of age

renal glomerulus atrophy ( J:82440 )

• glomerular tufts are retracted by 9 weeks of age

dilated renal tubule ( J:82440 )

• dilated tubules can be seen at 9 weeks of age

renal cast ( J:82440 )

• dilated tubules are packed with proteinaceous material in most places

pale kidney ( J:82440 )

• at 9 weeks of age

kidney failure ( J:82440 )

• develop end-stage kidney failure by 9-12 weeks of age

• no gross signs of renal failure prior to 7-8 weeks of age

integument

decreased skin turgor ( J:82440 )

cardiovascular system

abnormal glomerular capillary morphology ( J:82440 )

• no patent capillary loops can be seen at 9 weeks of age

abnormal glomerular capillary endothelium morphology ( J:82440 )

• at 2.5 weeks of age, mice display swelling of the endothelial cells (endotheliosis)

• endothelial cells become necrotic by 9 weeks of age

absent glomerular endothelium fenestra ( J:82440 )

• endothelial fenestrations are no longer identifiable by 9 weeks of age

• however, well-formed endothelial fenestrations are present at 2.5 weeks of age

Genotype
MGI:4938194

cn5

• Allelic Composition: Vegfa^tm2Gne/Vegfa⁺; Tg(SFTPC-rtTA)5Jaw/0; Tg(tetO-cre)1Jaw/0
• Genetic Background: involves: 129 * C57BL/6

respiratory system

decreased pulmonary endothelial cell surface ( J:124125 )

• at E18.5, doxycycline-exposed mice exhibit a reduction in pulmonary endothelial cell development that is intermediate between those of wild-type controls and those carrying two Vegfa^tm2Gne alleles

abnormal pulmonary circulation ( J:124125 )

• newborn mice exposed to doxycycline from E6.5 display pale lungs, indicating reduced pulmonary circulation

abnormal lung development ( J:124125 )

• mice exposed to doxycycline from E6.5 display defective distal lung saccular development due to moderately reduced primary septation

abnormal lung saccule morphology ( J:124125 )

• at E18.5, doxycycline-exposed mice display dilated distal airspaces of a size that is intermediate between those of wild-type controls and those carrying two Vegfa^tm2Gne alleles

• the mean linear intercept, a reflection of airspace size and hence an indirect measure of septation incidence, is inversely related to the Vegfa gene dosage

pale lung ( J:124125 )

• newborn mice exposed to doxycycline from E6.5 display pale lungs

cardiovascular system

decreased pulmonary endothelial cell surface ( J:124125 )

• at E18.5, doxycycline-exposed mice exhibit a reduction in pulmonary endothelial cell development that is intermediate between those of wild-type controls and those carrying two Vegfa^tm2Gne alleles

abnormal pulmonary circulation ( J:124125 )

• newborn mice exposed to doxycycline from E6.5 display pale lungs, indicating reduced pulmonary circulation

Genotype
MGI:4422199

cn6

• Allelic Composition: Vegfa^tm2Gne/Vegfa⁺; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CD-1 * SJL

vision/eye

abnormal retina morphology ( J:86207 )

• retinas that lack a proper outer vascular plexus are also thin compared to in wild-type mice

abnormal retina vasculature morphology ( J:86207 )

• at P21, retinas lack a proper outer vascular plexus unlike in wild-type mice

abnormal retina outer nuclear layer morphology ( J:86207 )

• between P5 and 3 weeks of age, mice lack clear development of the outer retinal layer compared to in wild-type mice

abnormal retina outer plexiform layer morphology ( J:86207 )

• at P21, mice lack clear development of the outer plexiform layer unlike in wild-type mice

retina degeneration ( J:86207 )

retina gliosis ( J:86207 )

• at P7, some retinas exhibit an increase in microglia/macrophage numbers near the developing veins compared to in wild-type mice

nervous system

abnormal brain vasculature morphology ( J:86207 )

• vascular density and sprouting angiogenesis in the cerebellum, brain stem, and spinal cord are decreased compared to in wild-type mice

• at P1, mice exhibit a decrease in the number of branch points of blood vessels in the forebrain and cortex while the distance between branch points is increased compared with wild-type mice

decreased brain size ( J:86207 )

• cortical brain size is reduced to in wild-type mice

decreased neuron number ( J:86207 )

• neuronal cellularity in the more superficial cortical layers I-III is decreased compared to in wild-type mice

cardiovascular system

abnormal brain vasculature morphology ( J:86207 )

• vascular density and sprouting angiogenesis in the cerebellum, brain stem, and spinal cord are decreased compared to in wild-type mice

• at P1, mice exhibit a decrease in the number of branch points of blood vessels in the forebrain and cortex while the distance between branch points is increased compared with wild-type mice

abnormal retina vasculature morphology ( J:86207 )

• at P21, retinas lack a proper outer vascular plexus unlike in wild-type mice

abnormal vascular branching morphogenesis ( J:86207 )

• at P1, mice exhibit a decrease in the number of branch points of blood vessels in the forebrain and cortex while the distance between branch points is increased compared with wild-type mice

decreased angiogenesis ( J:86207 )

• in the cerebellum, brain stem, and spinal cord