Vegfa^tm1Gne
Targeted Allele Detail

Summary

• Symbol: Vegfa^tm1Gne
• Name: vascular endothelial growth factor A; targeted mutation 1, Genentech
• MGI ID: MGI:1931041
• Synonyms: VEGF^-
• Gene: Vegfa Location: Chr17:46327919-46343295 bp, - strand Genetic Position: Chr17, 22.79 cM
• Alliance: Vegfa^tm1Gne page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:32218
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Replacement of exon 3 and flanking intronic sequence with a neomycin cassette. Heterozygous ES cells were shown to secrete half the wild-type amount of protein by radioreceptor assay. (J:32218)
• Inheritance: Dominant

Expression

In Mice Carrying this Mutation:	3 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vegfa Mutation: 37 strains or lines available

Notes

Homozygous null ES cells display a significantly impaired ability to form tumors in nude mice (J:32218).

References

• Original: J:32218 Ferrara N, et al., Heterozygous embryonic lethality induced by targeted inactivation of the VEGF gene. Nature. 1996 Apr 4;380(6573):439-42
• All: 1 reference(s)