Phenotypes associated with this allele
mortality/aging
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• die at E14.5 of severe dorsal edema and sometimes hemorrhage
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cardiovascular system
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• in one of two surviving mice
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• ventricular septation defects
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cellular
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• in one of two surviving mice
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mortality/aging
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• at P21 fewer mice are alive than wild type
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cardiovascular system
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• ventricular septation defects
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cellular
cardiovascular system
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• mice show normal numbers of epicardium-derived cells (EPDCs) and epicardial epithelial-mesenchymal transition (EMT) occurs normally
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Atoh7tm1Gla mutation
(1 available);
any
Atoh7 mutation
(9 available)
Gt(ROSA)26Sortm1Sho mutation
(4 available);
any
Gt(ROSA)26Sor mutation
(942 available)
Tg(Crx-Atoh7,-cre)251Gla mutation
(0 available)
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vision/eye
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• animals much thinner optic nerves relative to controls
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• in some animals, severe pathfinding defects are observed as the optic nerve exits the retina, forming a knot-like structure
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• cell death is increased relative to Atoh7-null homozygotes with most dying cells observed in the ganglion cell layer (GCL)
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• the transgenic Crx-Atoh7 (Math5) construct shows variable rescue of retinal axons and prevention of fasciculation defects in double mutants compared to Atoh7-null homozygotes
• rescue is less pronounced with successive generations
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nervous system
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• animals much thinner optic nerves relative to controls
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• in some animals, severe pathfinding defects are observed as the optic nerve exits the retina, forming a knot-like structure
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Atoh7tm1Gla mutation
(1 available);
any
Atoh7 mutation
(9 available)
Gt(ROSA)26Sortm1Sho mutation
(4 available);
any
Gt(ROSA)26Sor mutation
(942 available)
Tg(Crx-Atoh7,-cre)60Gla mutation
(0 available)
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vision/eye
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• knots of retinal ganglion cell axons are observed, consistent with the small degree of rescue
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• the BAC Crx-Atoh7 (Crx-Math5) construct shows some rescue of retinal axons and prevention of fasciculation defects in double mutants compared to Atoh7 homozygotes; level of rescue is lower than that seen in Atoh7-null homozygotes crossed to the conventional transgenic Atoh7 mice (Tg(Crx-Atoh7,-cre)251Gla)
• rescue is less pronounced with successive generations
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nervous system
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• knots of retinal ganglion cell axons are observed, consistent with the small degree of rescue
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1Sho mutation
(4 available);
any
Gt(ROSA)26Sor mutation
(942 available)
H2az2Tg(Wnt1-cre)11Rth mutation
(2 available);
any
H2az2 mutation
(26 available)
Nfatc1tm1Glm mutation
(0 available);
any
Nfatc1 mutation
(47 available)
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cardiovascular system
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• thinning of neural crest-derived mesenchyme in the distal outflow tract (dOFT) of E12.5 embryos, affecting the formation of the base of the aortic valve
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1Sho mutation
(4 available);
any
Gt(ROSA)26Sor mutation
(942 available)
Nfatc1tm1Glm mutation
(0 available);
any
Nfatc1 mutation
(47 available)
Tg(Tek-cre)1Ywa mutation
(6 available)
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cardiovascular system
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• boundary of the proximal outflow tract (pOFT) and distal outflow tract (dOFT) at the outflow tract bend is distrupted, with an extension of endocardium-derived mesenchyme into the dOFT cushion in mutants
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Brains of Tsc1tm1Djk/Tsc1tm1Djk Gt(ROSA)26Sortm1Sho/Gt(ROSA)26Sor+ mice injected with an adenovirus expressing cre recombinase show ventricular region abnormalities
mortality/aging
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• mice injected with a high dose of adenovirus expressing cre (AAVrh8-CBA-Cre) into each cerebral lateral ventricle on the day of birth exhibit a median survival of 38 days
• mice injected with a low dose of adenovirus expressing cre (AAVrh8-CBA-Cre) into each cerebral lateral ventricle on the day of birth exhibit a median survival of 180 days
• mice injected with a high dose of a different adenovirus expressing cre (AAV1-CBA-Cre) into each cerebral lateral ventricle on the day of birth exhibit a median survival of 66.5 days
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growth/size/body
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• mice injected with the AAV1-CBA-Cre adenovirus exhibit signs of distress at 1-5 months of age, including hunched back, dehydration, and weight loss
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nervous system
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• brains exhibit a smoother surface in mice injected with the AAV1-CBA-Cre adenovirus
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• severe hydrocephalus in 2 of 10 and mild hydrocephalus in 6 of 10 mice injected with AAV1-CBA-Cre
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• brains appear enlarged and swollen in 30 day old mice injected with the high dose of adenovirus (AAVrh8-CBA-Cre or AAV1-CBA-Cre) expressing cre
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• ventricular volume at P30 is about 4 times larger in mice injected with the AAV1-CBA-Cre adenovirus, while the brain tissue volume is about 6% larger
• 3 of 4 AAV1-CBA-Cre injected mice show nodules and thickening of the ventricular lining
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• massively enlarged lateral ventricles in mice injected with AAV1-CBA-Cre, resulting from a constriction between the 3rd and lateral ventricles
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• mice injected with AAV1-CBA-Cre develop hypertrophy of the subependymal layer, with expansion of the normal one cell thick layer into a convoluted layer with projections and isolated nodules
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• almost 2-fold increase in neuronal diameter near the periventricular area of 30 day old mice injected with the high dose of adenovirus expressing cre (AAVrh8-CBA-Cre or AAV1-CBA-Cre)
• some phospho-S6-positive Purkinje cells in the cerebellum and neurons in the caudate are enlarged in mice injected with AAV1-CBA-Cre
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behavior/neurological
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• mice injected with the high dose of adenovirus expressing cre (AAVrh8-CBA-Cre or AAV1-CBA-Cre) develop a hunched back
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Analysis Tools