All Phenotypes Wnt5a<tm1Amc> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Wnt5a^tm1Amc
targeted mutation 1, Andrew P McMahon
MGI:1857617

Summary

15 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Wnt5a^tm1Amc/Wnt5a^tm1Amc	involves: 129S7/SvEvBrd	MGI:4361494
hm2	Wnt5a^tm1Amc/Wnt5a^tm1Amc	involves: 129S7/SvEvBrd * C57BL/6	MGI:2653626
cn3	Daam1^tm1.1Tpy/Daam1^tm1.1Tpy Nkx2-5^tm1(cre)Rjs/Nkx2-5⁺ Wnt5a^tm1Amc/Wnt5a^tm1Amc	involves: 129S1/Sv * 129S7/SvEvBrd	MGI:6150926
cn4	Daam1^tm1.1Tpy/Daam1^tm1.1Tpy Nkx2-5^tm1(cre)Rjs/Nkx2-5⁺ Wnt5a^tm1Amc/Wnt5a⁺	involves: 129S1/Sv * 129S7/SvEvBrd	MGI:6151066
cn5	Wnt5a^tm1Amc/Wnt5a^tm1.1Krvl H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺	involves: 129S6/SvEvTac * 129S7/SvEvBrd * C57BL/6 * CBA/J	MGI:5605991
cx6	Fzd7^tm1.1Nat/Fzd7^tm1.1Nat Wnt5a^tm1Amc/Wnt5a⁺	involves: 129 * C57BL/6	MGI:5444717
cx7	Fzd2^tm1.1Nat/Fzd2⁺ Fzd7^tm1.1Nat/Fzd7^tm1.1Nat Wnt5a^tm1Amc/Wnt5a⁺	involves: 129 * C57BL/6	MGI:5444718
cx8	Wnt4^tm1Amc/Wnt4^tm1Amc Wnt5a^tm1Amc/Wnt5a⁺	involves: 129S1/Sv * 129S7/SvEvBrd	MGI:4412393
cx9	Wnt4^tm1Amc/Wnt4^tm1Amc Wnt5a^tm1Amc/Wnt5a^tm1Amc Wnt5b^tm1Tmj/Wnt5b^tm1Tmj	involves: 129S1/Sv * 129S7/SvEvBrd	MGI:4412392
cx10	Wnt4^tm1Amc/Wnt4⁺ Wnt5a^tm1Amc/Wnt5a^tm1Amc Wnt5b^tm1Tmj/Wnt5b^tm1Tmj	involves: 129S1/Sv * 129S7/SvEvBrd	MGI:4412398
cx11	Wnt4^tm1Amc/Wnt4^tm1Amc Wnt5a^tm1Amc/Wnt5a⁺ Wnt5b^tm1Tmj/Wnt5b^tm1Tmj	involves: 129S1/Sv * 129S7/SvEvBrd	MGI:4412399
cx12	Wnt4^tm1Amc/Wnt4^tm1Amc Wnt5a^tm1Amc/Wnt5a^tm1Amc Wnt5b^tm1Tmj/Wnt5b⁺	involves: 129S1/Sv * 129S7/SvEvBrd	MGI:4412400
cx13	Wnt4^tm1Amc/Wnt4^tm1Amc Wnt5a^tm1Amc/Wnt5a^tm1Amc	involves: 129S1/Sv * 129S7/SvEvBrd	MGI:4412395
cx14	Wnt4^tm1Amc/Wnt4⁺ Wnt5a^tm1Amc/Wnt5a^tm1Amc	involves: 129S1/Sv * 129S7/SvEvBrd	MGI:4412394
cx15	Wnt5a^tm1Amc/Wnt5a^tm1Amc Wnt5b^tm1Tmj/Wnt5b^tm1Tmj	involves: 129S7/SvEvBrd	MGI:4412397

Allelic Composition	Wnt5a^tm1Amc/Wnt5a^tm1Amc
Genetic Background	involves: 129S7/SvEvBrd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Wnt5a^tm1Amc mutation (1 available); any Wnt5a mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Primordial germ cell depletion in Ror2^Y324C/Ror2^Y324C and Wnt5a^tm1Amc/Wnt5a^tm1Amc mice

digestive/alimentary system

abnormal anus morphology ( J:153152 )

• at E18.5, all mice exhibit anorectal malformations and fail to show continuity of the rectum and anal epithelium unlike in wild-type mice

absent anus ( J:153152 )

anal atresia ( J:153152 )

• at E18.5

abnormal colon morphology ( J:153152 )

• at E15.5, mice exhibit a fistula between the bladder and the colon unlike wild-type mice

• at E18.5, only 25% of mice display a fistula between the bladder and colon unlike in wild-type mice

• most mice exhibit a blind-ending pouch without a fistula unlike in wild-type mice

decreased colon length ( J:153152 )

• mice exhibit a shortening of the colon

abnormal rectum morphology ( J:153152 )

• at E18.5, all mice exhibit anorectal malformations and fail to show continuity of the rectum and anal epithelium unlike in wild-type mice

absent rectum ( J:153152 )

• mice lack rectal tissues

renal/urinary system

abnormal urinary bladder morphology ( J:153152 )

• at E15.5, mice exhibit a fistula between the bladder and the colon unlike wild-type mice

• at E18.5, only 25% of mice display a fistula between the bladder and colon unlike in wild-type mice

• most mice exhibit a blind-ending pouch without a fistula unlike in wild-type mice

liver/biliary system

abnormal liver morphology ( J:153152 )

• the liver is displaced caudally

enlarged liver ( J:153152 )

limbs/digits/tail

decreased autopod size ( J:184918 )

• truncated

short limbs ( J:153152 , J:184918 )

short tail ( J:153152 )

reproductive system

decreased primordial germ cell number ( J:179805 )

• at E10.5, E11.5 and E12.5

increased primordial germ cell apoptosis ( J:179805 )

• increase in apoptosis of extragonadal primordial germ cells at E10.5 and E11.5

abnormal primordial germ cell migration ( J:179805 )

• fail to migrate rostrally at E10.5, remaining caudally distributed

• ectopic PGCs make up over 70% of the total PGCs at E11.5 compared to less than 5% in wild-type controls

genital tubercle hypoplasia ( J:153152 )

• at E18.5, the genital tubercle is hypoplastic compared to in wild-type mice

growth/size/body

decreased body size ( J:153152 )

enlarged liver ( J:153152 )

nervous system

nervous system phenotype ( J:155074 )

N	• mice exhibit normal numbers of thoracic motor neurons and proportions of motor columnar subtypes

cellular

decreased primordial germ cell number ( J:179805 )

• at E10.5, E11.5 and E12.5

increased primordial germ cell apoptosis ( J:179805 )

• increase in apoptosis of extragonadal primordial germ cells at E10.5 and E11.5

abnormal primordial germ cell migration ( J:179805 )

• fail to migrate rostrally at E10.5, remaining caudally distributed

• ectopic PGCs make up over 70% of the total PGCs at E11.5 compared to less than 5% in wild-type controls

Allelic Composition	Wnt5a^tm1Amc/Wnt5a^tm1Amc
Genetic Background	involves: 129S7/SvEvBrd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Wnt5a^tm1Amc mutation (1 available); any Wnt5a mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:52600 , J:78384 )

• live at birth but dead shortly thereafter (J:52600)

craniofacial

short mandible ( J:52600 )

decreased tongue size ( J:52600 )

• tongue truncated

short snout ( J:52600 )

small ears ( J:52600 )

• reduced outgrowth of external ears

embryo

abnormal embryonic tissue morphology ( J:52600 )

• embryos examined at E17.5 to E18.5 showed gross morphological defects of outgrowing tissues

abnormal mesenchyme morphology ( J:204986 )

• ventral outgrowth of the dorsal mesenchyme is arrested unlike in wild-type mice

• however, the dorsal mesenchyme does form

abnormal presomitic mesoderm morphology ( J:52600 )

• reduction in presomitic mesoderm, evident at E9.5

abnormal primitive streak formation ( J:52600 )

• shortened, evident at E8.5

decreased somite size ( J:52600 )

• at E9.5, evident that caudal somites were smaller than controls

• same number of somites existed, however

growth/size/body

decreased tongue size ( J:52600 )

• tongue truncated

short snout ( J:52600 )

small ears ( J:52600 )

• reduced outgrowth of external ears

decreased body length ( J:52600 )

• embryos examined at E17.5 to E18.5 were truncated caudally

• significant shortening of embryonic anterior-posterior axis

hearing/vestibular/ear

small ears ( J:52600 )

• reduced outgrowth of external ears

limbs/digits/tail

adactyly ( J:52600 )

• of both fore- and hindlimbs

short humerus ( J:52600 )

• 59% the length of littermate controls

short radius ( J:52600 )

• 30 to 40% the length of littermate controls

short ulna ( J:52600 )

• 30 to 40% the length of littermate controls

short limbs ( J:52600 )

absent caudal vertebrae ( J:52600 )

vestigial tail ( J:52600 )

reproductive system

abnormal reproductive system morphology ( J:52600 )

absent external female genitalia ( J:52600 )

absent external male genitalia ( J:52600 )

respiratory system

abnormal lung development ( J:78384 )

• hypercellular with thickened interstitium, evident at E18

• increased numbers of terminal airways, evident at E16

• overexpanded distal airways

• failed coupling of airways to lung capillaries

• differentiation of specialized lung epithelial cells was normal, however

abnormal lung interstitium morphology ( J:78384 )

• thickened, evident at E18

decreased tracheal cartilage ring number ( J:78384 )

• fewer cartilage rings than in littermate controls

short trachea ( J:78384 )

• short, with fewer cartilage rings than littermate controls

• no evidence of tracheoesophogeal fusion or of abnormal cellular differentation

respiratory failure ( J:78384 )

• neonatal death apparently due to respiratory failure

skeleton

short mandible ( J:52600 )

short humerus ( J:52600 )

• 59% the length of littermate controls

short radius ( J:52600 )

• 30 to 40% the length of littermate controls

short ulna ( J:52600 )

• 30 to 40% the length of littermate controls

decreased tracheal cartilage ring number ( J:78384 )

• fewer cartilage rings than in littermate controls

rib fusion ( J:52600 )

abnormal vertebrae morphology ( J:52600 )

• abnormalities increased in severity caudally

absent caudal vertebrae ( J:52600 )

small vertebrae ( J:52600 )

vertebral fusion ( J:52600 )

short vertebral column ( J:78384 )

• length of vertebral column was 1/2 that of littermate controls

delayed bone ossification ( J:52600 )

• in proximal limb elements

• absent in radius and metacarpals

digestive/alimentary system

decreased tongue size ( J:52600 )

• tongue truncated

abnormal digestive system development ( J:204986 )

• ventral outgrowth of the dorsal mesenchyme is arrested unlike in wild-type mice

• however, the dorsal mesenchyme does form

abnormal midgut morphology ( J:204986 )

• mice fail to initiate the leftward tilt of the midgut unlike wild-type mice

Allelic Composition	Daam1^tm1.1Tpy/Daam1^tm1.1Tpy Nkx2-5^tm1(cre)Rjs/Nkx2-5⁺ Wnt5a^tm1Amc/Wnt5a^tm1Amc
Genetic Background	involves: 129S1/Sv * 129S7/SvEvBrd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Daam1^tm1.1Tpy mutation (0 available); any Daam1 mutation (77 available)
Nkx2-5^tm1(cre)Rjs mutation (1 available); any Nkx2-5 mutation (21 available)
Wnt5a^tm1Amc mutation (1 available); any Wnt5a mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cardiovascular system

abnormal heart morphology ( J:228507 )

• right ventricle shifted anterior to left ventricle in E12.5 embryos

• AV cushion atop single ventricle connected to both atria in some embryos in E12.5 embryos

abnormal myocardial trabeculae morphology ( J:228507 )

• rudimentary in left ventricle of E16.5 embryos

abnormal myocardium compact layer morphology ( J:228507 )

• rudimentary in left ventricle of E16.5 embryos

abnormal interventricular septum morphology ( J:228507 )

• shorter than controls, not extended to the endocardial cushion in the atrioventricular canal in E12.5 embryos

abnormal heart right ventricle morphology ( J:228507 )

• mesenchyme-like organization of cardiomyocytes at ventricle base in E12.5 embryos

• right ventricle shifted anterior to left ventricle in E12.5 embryos

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:228507 )

• very few embryos seen at E16.5 and those that are, are mostly necrotic

muscle

abnormal myocardial trabeculae morphology ( J:228507 )

• rudimentary in left ventricle of E16.5 embryos

abnormal myocardium compact layer morphology ( J:228507 )

• rudimentary in left ventricle of E16.5 embryos

Allelic Composition	Daam1^tm1.1Tpy/Daam1^tm1.1Tpy Nkx2-5^tm1(cre)Rjs/Nkx2-5⁺ Wnt5a^tm1Amc/Wnt5a⁺
Genetic Background	involves: 129S1/Sv * 129S7/SvEvBrd

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cardiovascular system

abnormal myocardial trabeculae morphology ( J:228507 )

• thicker right ventricular trabecular zones, extending into lumen in E16.5 embryos

thick myocardium compact layer ( J:228507 )

• in right ventricle of E16.5 embryos

muscle

abnormal myocardial trabeculae morphology ( J:228507 )

• thicker right ventricular trabecular zones, extending into lumen in E16.5 embryos

thick myocardium compact layer ( J:228507 )

• in right ventricle of E16.5 embryos

Allelic Composition	Wnt5a^tm1Amc/Wnt5a^tm1.1Krvl H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺
Genetic Background	involves: 129S6/SvEvTac * 129S7/SvEvBrd * C57BL/6 * CBA/J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
H2az2^{Tg(Wnt1-cre)11Rth} mutation (2 available); any H2az2 mutation (26 available)
Wnt5a^tm1.1Krvl mutation (1 available); any Wnt5a mutation (40 available)
Wnt5a^tm1Amc mutation (1 available); any Wnt5a mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:196291 )

• pups die within a few hours of birth

nervous system

increased neuron apoptosis ( J:196291 )

• increased neuron apoptosis observed in superior cervical ganglia

abnormal axon extension ( J:196291 )

• defects in extension and branching of sympathetic axons are observed in salivary glands, thymus, heart, spleen and bladder at E16.5

abnormal sympathetic neuron innervation pattern ( J:196291 )

• incomplete innervation in paravertebral sympathetic ganglia and prevertebral ganglia

• sympathetic axons in spleen extend to target, but fail to grow and arborize spleen parenchyma

craniofacial

abnormal craniofacial morphology ( J:196291 )

• pups exhibit craniofacial abnormalities

cellular

increased neuron apoptosis ( J:196291 )

• increased neuron apoptosis observed in superior cervical ganglia

abnormal axon extension ( J:196291 )

• defects in extension and branching of sympathetic axons are observed in salivary glands, thymus, heart, spleen and bladder at E16.5

Allelic Composition	Fzd7^tm1.1Nat/Fzd7^tm1.1Nat Wnt5a^tm1Amc/Wnt5a⁺
Genetic Background	involves: 129 * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fzd7^tm1.1Nat mutation (1 available); any Fzd7 mutation (35 available)
Wnt5a^tm1Amc mutation (1 available); any Wnt5a mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

limbs/digits/tail

short tail ( J:189062 )

• truncation is more severe than in mice homozygous for Fzd7^tm1.1Nat alone

Allelic Composition	Fzd2^tm1.1Nat/Fzd2⁺ Fzd7^tm1.1Nat/Fzd7^tm1.1Nat Wnt5a^tm1Amc/Wnt5a⁺
Genetic Background	involves: 129 * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fzd2^tm1.1Nat mutation (1 available); any Fzd2 mutation (34 available)
Fzd7^tm1.1Nat mutation (1 available); any Fzd7 mutation (35 available)
Wnt5a^tm1Amc mutation (1 available); any Wnt5a mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:189062 )

• all die by E11

growth/size/body

embryonic growth retardation ( J:189062 )

• starting at E9.5

embryo

embryonic growth retardation ( J:189062 )

• starting at E9.5

Allelic Composition	Wnt4^tm1Amc/Wnt4^tm1Amc Wnt5a^tm1Amc/Wnt5a⁺
Genetic Background	involves: 129S1/Sv * 129S7/SvEvBrd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Wnt4^tm1Amc mutation (2 available); any Wnt4 mutation (20 available)
Wnt5a^tm1Amc mutation (1 available); any Wnt5a mutation (40 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal motor neuron morphology ( J:155074 )

• at E13.5, median motor column motor neurons at the thoracic level are decreased 46% compared to in wild-type mice

• the ratio of median motor column motor neurons at the hind limb level is decreased compared to in wild-type mice

• at E13.5, hypaxial motor column motor neurons at the thoracic level are increased compared to in wild-type mice

Allelic Composition	Wnt4^tm1Amc/Wnt4^tm1Amc Wnt5a^tm1Amc/Wnt5a^tm1Amc Wnt5b^tm1Tmj/Wnt5b^tm1Tmj
Genetic Background	involves: 129S1/Sv * 129S7/SvEvBrd

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:155074 )

• embryos die before the onset of motor neuron differentiation

Allelic Composition	Wnt4^tm1Amc/Wnt4⁺ Wnt5a^tm1Amc/Wnt5a^tm1Amc Wnt5b^tm1Tmj/Wnt5b^tm1Tmj
Genetic Background	involves: 129S1/Sv * 129S7/SvEvBrd

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal motor neuron morphology ( J:155074 )

• at E13.5, median motor column motor neurons at the thoracic level are decreased 59% compared to in wild-type mice

• at E13.5, hypaxial motor column motor neurons at the thoracic level are increased 62% compared to in wild-type mice

limbs/digits/tail

abnormal limb morphology ( J:155074 )

Allelic Composition	Wnt4^tm1Amc/Wnt4^tm1Amc Wnt5a^tm1Amc/Wnt5a⁺ Wnt5b^tm1Tmj/Wnt5b^tm1Tmj
Genetic Background	involves: 129S1/Sv * 129S7/SvEvBrd

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal motor neuron morphology ( J:155074 )

• at E13.5, median motor column motor neurons at the thoracic level are decreased 63% compared to in wild-type mice

• mice exhibit a 50% reduction in median motor column motor neurons at the lumbar level compared with wild-type mice

• at the hind limb level, the total number of motor neurons is decreased 20% while the ratio of median motor column motor neurons is decreased 50% compared to in wild-type mice

• at E13.5, hypaxial motor column motor neurons at the thoracic level are increased 50% compared to in wild-type mice

decreased motor neuron number ( J:155074 )

• at the hind limb level, the total number of motor neurons is decreased 20% compared to in wild-type mice

limbs/digits/tail

abnormal limb morphology ( J:155074 )

Allelic Composition	Wnt4^tm1Amc/Wnt4^tm1Amc Wnt5a^tm1Amc/Wnt5a^tm1Amc Wnt5b^tm1Tmj/Wnt5b⁺
Genetic Background	involves: 129S1/Sv * 129S7/SvEvBrd

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal motor neuron morphology ( J:155074 )

• at E13.5, median motor column motor neurons at the thoracic level are decreased 67% compared to in wild-type mice

• at E13.5, hypaxial motor column motor neurons at the thoracic level are increased 39% compared to in wild-type mice

limbs/digits/tail

abnormal limb morphology ( J:155074 )

Allelic Composition	Wnt4^tm1Amc/Wnt4^tm1Amc Wnt5a^tm1Amc/Wnt5a^tm1Amc
Genetic Background	involves: 129S1/Sv * 129S7/SvEvBrd

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal motor neuron morphology ( J:155074 )

• at E13.5, median motor column motor neurons at the thoracic level are decreased 58% compared to in wild-type mice

• at E13.5, hypaxial motor column motor neurons at the thoracic level are increased compared to in wild-type mice

limbs/digits/tail

abnormal limb morphology ( J:155074 )

Allelic Composition	Wnt4^tm1Amc/Wnt4⁺ Wnt5a^tm1Amc/Wnt5a^tm1Amc
Genetic Background	involves: 129S1/Sv * 129S7/SvEvBrd

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

decreased motor neuron number ( J:155074 )

• at E13.5, median motor column motor neurons at the thoracic level are decreased 22% compared to in wild-type mice

limbs/digits/tail

abnormal limb morphology ( J:155074 )

Allelic Composition	Wnt5a^tm1Amc/Wnt5a^tm1Amc Wnt5b^tm1Tmj/Wnt5b^tm1Tmj
Genetic Background	involves: 129S7/SvEvBrd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Wnt5a^tm1Amc mutation (1 available); any Wnt5a mutation (40 available)
Wnt5b^tm1Tmj mutation (0 available); any Wnt5b mutation (35 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal motor neuron morphology ( J:155074 )

• at E13.5, median motor column motor neurons at the thoracic level are decreased 50% compared to in wild-type mice

• at E13.5, hypaxial motor column motor neurons at the thoracic level are increased compared to in wild-type mice

limbs/digits/tail

abnormal limb morphology ( J:155074 )

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