Tg(Tyr)SpotNpln
Transgene Detail

Summary

• Symbol: Tg(Tyr)SpotNpln
• Name: transgene insertion Spot, Nicolas Pilon
• MGI ID: MGI:8211391
• Synonyms: Spot^Tg
• Transgene: Tg(Tyr)SpotNpln Location: unknown Genetic Position: Chr13, Syntenic
• Alliance: Tg(Tyr)SpotNpln page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Modified regulatory region)
• Mutations: Insertion, Intergenic deletion
• Tg(Tyr)SpotNpln expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tyr (MGI:98880)

• Mutation details: Around 17-20 copies of a tyrosinase minigene (consisting of the Tyr distal enhancer in reverse orientation upstream of the proximal promoter and complete Tyr cDNA) replaced a 353-bp sequence in an A830082K12Rik intron near Nr2f1 and A830082K12Rik silencer Tg(Gt(ROSA)26Sor-EGFP/Arrb2)1Svs transgene, but that transgene is not expressed does not seem to have integrated. Insertion of the transgene disrupts the silencer activity, leading to increased transcription of the Nr2f1 and A830082K12Rik genes in neural crest-derived cells, including enteric neural crest cells (ENCCs) and Schwann cell precursors (SCPs) of E12.5 embryos. This Spot line was generated in a forward genetic screen aimed at identifying genes with key roles in neural crest cells. (J:236786)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:236786 Bergeron KF, et al., Upregulation of the Nr2f1-A830082K12Rik gene pair in murine neural crest cells results in a complex phenotype reminiscent of Waardenburg syndrome type 4. Dis Model Mech. 2016 Nov 1;9(11):1283-1293
• All: 1 reference(s)