Phenotypes associated with this allele

• Allele Symbol: Tg(Tyr)SpotNpln
• Allele Name: transgene insertion Spot, Nicolas Pilon
• Allele ID: MGI:8211391
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(Gata4-GFP)6AVigr/0 Tg(Tyr)SpotNpln/Tg(Tyr)SpotNpln	FVB/N-Tg(Arrb2/GFP)#Npln Tg(Gata4-GFP)6Avigr Tg(Tyr)SpotNpln	MGI:8213741
cx2	Tg(Gata4-RFP)#Vigr/0 Tg(Tyr)SpotNpln/Tg(Tyr)SpotNpln	FVB/N-Tg(Arrb2/GFP)#Npln Tg(Gata4-RFP)#Vigr Tg(Tyr)SpotNpln	MGI:8213743
tg3	Tg(Tyr)SpotNpln/Tg(Tyr)SpotNpln	FVB/N-Tg(Arrb2/GFP)#Npln Tg(Tyr)SpotNpln	MGI:8213737

Genotype
MGI:8213741

cx1

• Allelic Composition: Tg(Gata4-GFP)6AVigr/0; Tg(Tyr)SpotNpln/Tg(Tyr)SpotNpln
• Genetic Background: FVB/N-Tg(Arrb2/GFP)#Npln Tg(Gata4-GFP)6Avigr Tg(Tyr)SpotNpln

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

circling ( J:236786 )

• at age P20

cellular

abnormal glial cell physiology ( J:236786 )

• premature differentiation of enteric neural crest cells (ENCCs) towards glial lineage in proximal colon in E15.5 embryos and proximal small intestine in E13.5 embryos

digestive/alimentary system

aganglionic megacolon ( J:236786 )

• at age P20

integument

absent coat pigmentation ( J:236786 )

• at age P20

nervous system

abnormal glial cell physiology ( J:236786 )

• premature differentiation of enteric neural crest cells (ENCCs) towards glial lineage in proximal colon in E15.5 embryos and proximal small intestine in E13.5 embryos

pigmentation

absent coat pigmentation ( J:236786 )

• at age P20

Genotype
MGI:8213743

cx2

• Allelic Composition: Tg(Gata4-RFP)#Vigr/0; Tg(Tyr)SpotNpln/Tg(Tyr)SpotNpln
• Genetic Background: FVB/N-Tg(Arrb2/GFP)#Npln Tg(Gata4-RFP)#Vigr Tg(Tyr)SpotNpln

behavior/neurological

circling ( J:236786 )

• at age P20

cellular

abnormal glial cell physiology ( J:236786 )

• premature differentiation of enteric neural crest cells (ENCCs) towards glial lineage in proximal colon in E15.5 embryos and proximal small intestine in E13.5 embryos

digestive/alimentary system

aganglionic megacolon ( J:236786 )

• at age P20

integument

absent coat pigmentation ( J:236786 )

• at age P20

nervous system

abnormal glial cell physiology ( J:236786 )

• premature differentiation of enteric neural crest cells (ENCCs) towards glial lineage in proximal colon in E15.5 embryos and proximal small intestine in E13.5 embryos

pigmentation

absent coat pigmentation ( J:236786 )

• at age P20

Genotype
MGI:8213737

tg3

• Allelic Composition: Tg(Tyr)SpotNpln/Tg(Tyr)SpotNpln
• Genetic Background: FVB/N-Tg(Arrb2/GFP)#Npln Tg(Tyr)SpotNpln

behavior/neurological

circling ( J:236786 )

• at age P20

digestive/alimentary system

aganglionic megacolon ( J:236786 )

• at age P20

hearing/vestibular/ear

hearing/vestibular/ear phenotype ( J:236786 )

N • normal hearing at age P21

small endolymphatic duct ( J:236786 )

• absence of endolymph liquid and collapsed cellular membrane in inner ear at age P20

integument

absent coat pigmentation ( J:236786 )

• at age P20

limbs/digits/tail

abnormal forelimb morphology ( J:236786 )

• dropping forelimbs

mortality/aging

neonatal lethality, incomplete penetrance ( J:236786 )

• most newborns die between P1 and P2

nervous system

abnormal hypoglossal nerve morphology ( J:236786 )

• abnormal connection with spinal nerve I at right side in 71% of E10.5 embryos

abnormal spinal nerve morphology ( J:236786 )

• abnormal connection with cranial nerve XII (hypoglossal) at right side in 71% of E10.5 embryos

pigmentation

absent coat pigmentation ( J:236786 )

• at age P20

decreased melanocyte number ( J:236786 )

• absent vestibular melanocytes at age P20

skeleton

abnormal spine curvature ( J:236786 )

• abnormal body curvature