Col1a1^{tm11(tetO-Dnmt3b_i6)Jae}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm11(tetO-Dnmt3b_i6)Jae}
• Name: collagen, type I, alpha 1; targeted mutation 11, Rudolf Jaenisch
• MGI ID: MGI:5313407
• Synonyms: tetO-Dnmt3b6
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm11(tetO-Dnmt3b_i6)Jae} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127808
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm11(tetO-Dnmt3b_i6)Jae} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Dnmt3b (MGI:1261819)

• Col1a1^{tm11(tetO-Dnmt3b_i6)Jae} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: RMCE on KH2 cells inserted a cassette that contains a splice acceptor-double polyA sequence and the tetracycline responsive element (tetO) upstream of the DNA methyltransferase 3B gene isoform 6 (Dnmt3b) into the 3'UTR. (J:127808)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:127808 Linhart HG, et al., Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev. 2007 Dec 1;21(23):3110-22
• All: 1 reference(s)