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Gt(ROSA)26Sortm1(rtTA*M2)Jae
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm1(rtTA*M2)Jae
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Rudolf Jaenisch
MGI ID: MGI:3702294
Synonyms: Gt(ROSA)26Sortm1(M2rtTA)Jae, M2, M2-rtTA, R26-M2rtTA, R26-rtTA, Rosa26-M2rtTA
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm1(rtTA*M2)Jae page
Col1a1tm1(tetO-Tcfap2c)Hsc/Col1a1+ Gt(ROSA)26Sortm1(rtTA*M2)Jae/Gt(ROSA)26Sor+ mice show increased proliferation and induced hepatic steatosis

Show the 4 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:98920
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Transactivator)
Mutation:    Insertion
 
Mutation detailsAn optimized form of reverse tetracycline controlled transactivator (rtTA-M2) was inserted downstream of the Gt(ROSA)26Sor promoter and was followed by a PGK-puro selection cassette. This mutant form of rtTA termed M2 has five amino acid substitutions in the tetR moiety of tTA: S12G, E19G, A56P, D148E and H179R. This mutated form of transactivator protein has increased doxycycline sensitivity. Mice have widespread expression of the rtTA-M2 protein. (J:98920)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 30 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  935 strains or lines available
Notes
This mutation was created during the generation of mutant ES cell line KH2, which also contains a frt docking site downstream of the endogenous Col1a1 locus on Chr 11 (Col1a1). The two mutations have subsequently been bred apart.
References
Original:  J:98920 Hochedlinger K, et al., Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell. 2005 May 6;121(3):465-77
All:  284 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory