Gt(ROSA)26Sor<tm1(rtTA*M2)Jae> Targeted Allele Detail MGI Mouse (MGI:3702294)

Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}
Targeted Allele Detail

Summary

Symbol:	Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Rudolf Jaenisch
MGI ID:	MGI:3702294
Synonyms:	Gt(ROSA)26Sor^{tm1(M2rtTA)Jae}, M2, M2-rtTA, R26-M2rtTA, R26-rtTA, Rosa26-M2rtTA
Gene:	Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} page

Col1a1^{tm1(tetO-Tcfap2c)Hsc}/Col1a1⁺ Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/Gt(ROSA)26Sor⁺ mice show increased proliferation and induced hepatic steatosis

Show the 4 phenotype image(s) involving this allele.

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:98920
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:		Targeted (Transactivator)
Mutation:		Insertion
		Mutation details: An optimized form of reverse tetracycline controlled transactivator (rtTA-M2) was inserted downstream of the Gt(ROSA)26Sor promoter and was followed by a PGK-puro selection cassette. This mutant form of rtTA termed M2 has five amino acid substitutions in the tetR moiety of tTA: S12G, E19G, A56P, D148E and H179R. This mutated form of transactivator protein has increased doxycycline sensitivity. Mice have widespread expression of the rtTA-M2 protein. (J:98920)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 32 strains available Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	1046 strains or lines available

Notes

This mutation was created during the generation of mutant ES cell line KH2, which also contains a FRT docking site downstream of the endogenous Col1a1 locus on Chr 11 (Col1a1). The two mutations have subsequently been bred apart.

References

Original:	J:98920 Hochedlinger K, et al., Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell. 2005 May 6;121(3):465-77
All:	345 reference(s)

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