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Targeted Allele Detail
Symbol: Col1a1tm2(tetO-LIN28B)Gqda
Name: collagen, type I, alpha 1; targeted mutation 2, George Q Daley
MGI ID: MGI:5294612
Synonyms: iLIN28B
Gene: Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance: Col1a1tm2(tetO-LIN28B)Gqda page
Germline Transmission:  Earliest citation of germline transmission: J:177113
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Allele Type:    Targeted (Humanized sequence, Inducible, Inserted expressed sequence, RMCE-ready)
Inducer:    doxycycline/tetracycline
Mutation:    Insertion
Col1a1tm2(tetO-LIN28B)Gqda expresses 1 gene
Mutation detailsRMCE on KH2 cells inserted a construct, which contains (5' to 3') a splice acceptor, 2 polyadenylation sequence, tetracycline operator, a flag-tagged human LIN28B cDNA, and an SV40 polyadenylation signal, into the 3'UTR. No expression of the inserted gene is detected in the absence of doxycycline treatment. (J:177113)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  140 strains or lines available
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae (J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae.
Original:  J:177113 Zhu H, et al., The Lin28/let-7 Axis Regulates Glucose Metabolism. Cell. 2011 Sep 30;147(1):81-94
All:  17 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
MGI 6.22
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