Tmprss13^tm1Dgen
Targeted Allele Detail

Summary

• Symbol: Tmprss13^tm1Dgen
• Name: transmembrane protease, serine 13; targeted mutation 1, Deltagen
• MGI ID: MGI:3663353
• Synonyms: Tmprss13^-
• Gene: Tmprss13 Location: Chr9:45230398-45258879 bp, + strand Genetic Position: Chr9, 24.84 cM
• Alliance: Tmprss13^tm1Dgen page

Mutation origin

• Mutant Cell Line: 7908
• Germline Transmission: Earliest citation of germline transmission: J:101679
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Deltagen

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A 4 kb region, including the last 92 bp of exon 9 and the entire exon 10, was replaced with a promoterless LacZ-Neo fusion gene (beta-galactosidase reporter). This deletion includes exon 9 sequences that encode Asp395, which forms part of the catalytic His-Asp-Ser triad. The in-frame insertion of the LacZ-Neo fusion gene leads to the expression of a fusion protein consisting of the first 377 amino acids fused to beta-galactosidase. High and moderate levels of RNA transcripts were detected in urinary bladder, skin, tongue, seminal vesicle, coagulating gland, and prostate gland. No RNA transcripts are detectable in nervous tissue, liver, bone marrow, small intestine, or cecum. LacZ expression was detected in many tissues examined, particularly in the epidermis and urinary bladder. No LacZ expression was detected in spinal cord, sciatic nerve, spleen, lymph nodes, bone marrow, aorta, liver, pancreas, pituitary gland, adrenal gland, skeletal muscle, testis, ovary, or uterus. Detailed X-gal staining analysis of young adult heterozygotes revealed particularly strong expression in multi-layered squamous epithelium, including the epidermis, hair follicles, oral epithelium, cornea and upper digestive tract, high levels of expression in the transitional epithelium of the bladder, and much lower levels in the prostate, heart, intestine, kidney and thymus. (J:101679, J:215250)

Expression

In Mice Carrying this Mutation:	40 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tmprss13 Mutation: 24 strains or lines available

Notes

See also, data as provided by Deltagen, Inc.

References

• Original: J:101679 Deltagen Inc, NIH initiative supporting placement of Deltagen, Inc. mice into public repositories. MGI Direct Data Submission. 2005
• All: 3 reference(s)