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Gene Expression Data
Assay Details
Assay
Reference: J:355262 Kadhim AZ, et al., Transcriptional coactivator MED15 is required for beta cell maturation. Nat Commun. 2024 Oct 8;15(1):8711
Assay type: Immunohistochemistry
MGI Accession ID: MGI:8166933
Gene symbol: Med15
Gene name: mediator complex subunit 15
Antibody: anti-Med15
Results
Specimen 1B Med15: embryonic day 13.5 (more )
Note: Triple labeled: green - Med15; white - Gcg (assay MGI:8166935); red - Ins1 (assay MGI:8166934) and Ins2 (assay MGI:8166967).
Structure Cell Type Level Pattern Image Note
TS21: pancreas type B pancreatic cell Strong Regionally restricted 1B (upper) , 1B (lower) High nuclear expression in nascent insulin-expressing beta-cells.
TS21: pancreas pancreatic A cell Absent 1B (upper) , 1B (lower) No expression in glucagon-expressing alpha-cells.
Specimen 1C Med15: embryonic day 18.5 (more )
Note: Triple labeled: green - Med15; white - Gcg (assay MGI:8166935); red - Ins1 (assay MGI:8166934) and Ins2 (assay MGI:8166967).
Structure Cell Type Level Pattern Image Note
TS26: pancreas type B pancreatic cell Present Regionally restricted 1C (upper) , 1C (lower) Nuclear expression in some beta-cells and in alpha-cells.
TS26: pancreas pancreatic A cell Present 1C (upper) , 1C (lower) Expression in alpha-cells.
Specimen 1D Med15: postnatal week 8 (more )
Note: Triple labeled: green - Med15; white - Gcg (assay MGI:8166935); red - Ins1 (assay MGI:8166934) and Ins2 (assay MGI:8166967).
Structure Cell Type Level Pattern Image Note
TS28: pancreas pancreatic A cell Present Regionally restricted 1D (lower) , 1D (upper) Nuclear expression in both alpha-cells and beta-cells.
TS28: pancreas type B pancreatic cell Present Regionally restricted 1D (lower) , 1D (upper) Nuclear expression in both alpha-cells and beta-cells.
Specimen 2B CTRL Med15: postnatal week 8; Ins1tm1.1(cre)Thor/Ins1+ (more )
Note: Double labeled: green - Med15; red - Ins1 (assay MGI:8166934) and Ins2 (assay MGI:8166967).
Structure Level Pattern Image Note
TS28: pancreas Present 2B CTRL
Specimen 2B M15betaKO Med15: postnatal week 8; Ins1tm1.1(cre)Thor/Ins1+, Med15tm1c(KOMP)Wtsi/Med15tm1c(KOMP)Wtsi (more )
Note: Conditional mutant. Double labeled: green - Med15; red - Ins1 (assay MGI:8166934) and Ins2 (assay MGI:8166967).
Structure Level Pattern Image Note
TS28: pancreas Absent 2B M15betaKO Authors report beta-cell-specific knockout.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/06/2026
MGI 6.29 		The Jackson Laboratory