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Gene Expression Data
Assay Details
Assay
Reference: J:279927 Batiuk MY, et al., An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody. J Biol Chem. 2017 May 26;292(21):8874-8891
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7619762
Gene symbol: Atp1b2
Gene name: ATPase, Na+/K+ transporting, beta 2 polypeptide
Antibody: ACSA-2
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 9A top: postnatal day 21 (more )
Note: Double labeled: magenta - Atp1b2; green - Slc1a2 (assay MGI:7619788).
Structure Cell Type Level Pattern Image Note
TS28: visual cortex astrocyte of the cerebral cortex Present 9A ACSA-2 top , 9A Merge top Astrocyte staining pattern was highly similar to Slc1a2 immunostaining although non-overlapping.
Specimen 9A bottom: postnatal day 21 (more )
Note: Double labeled: magenta - Atp1b2; green - Map2 (assay MGI:7619790).
Structure Cell Type Level Pattern Image Note
TS28: visual cortex cerebral cortex neuron Absent 9A Merge bottom , 9A ACSA-2 bottom Staining pattern was highly dissimilar to the neuronal microtubule marker Map2 immunostaining.
TS28: visual cortex astrocyte of the cerebral cortex Present 9A ACSA-2 bottom , 9A Merge bottom
Specimen 9B bottom: postnatal day 21 (more )
Note: Double labeled: magenta - Atp1b2; green - Slc1a2 (assay MGI:7619788).
Structure Cell Type Level Pattern Image Note
TS28: corpus callosum astrocyte of the cerebral cortex Present 9B Merge bottom , 9B ACSA-2 bottom ACSA-2 signal was detected around astrocyte cell bodies and extended into astrocyte processes. Astrocyte Atp1b2 immunostaining and Slc1a2 immunostaining colocalize to the same cell, albeit in non-overlapping domains.
Specimen 10B row 1/2: postnatal month 6; Apptm3.1Tcs/Apptm3.1Tcs (more )
Note: Double labeled: magenta - Atp1b2; green - Aif1 (assay MGI:7619797).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex astrocyte of the cerebral cortex Present Regionally restricted 10B ACSA-2 (low mag row 1) , 10B ACSA-2 (high mag row 2) , 10B Merge (high mag row 2) The plaques in App<NL-G-F> cortex exclude astrocytes, and are devoid of staining. Astrocytes immediately adjacent to the plaques show immunoreactivity.
Specimen 10B row 3: postnatal month 6; Apptm3.1Tcs/Apptm3.1Tcs (more )
Note: Double labeled: magenta - Atp1b2; green - Gfap (assay MGI:7619800).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex astrocyte of the cerebral cortex Present Regionally restricted 10B Merge (high mag row 3) , 10B ACSA-2 (high mag row 3) The plaques in App<NL-G-F> cortex exclude astrocytes, and are devoid of staining. Astrocytes immediately adjacent to the plaques show immunoreactivity.
Specimen 10B row 4: postnatal month 6; Apptm3.1Tcs/Apptm3.1Tcs (more )
Note: Double labeled: magenta - Atp1b2; green - S100b (assay MGI:7619801).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex astrocyte of the cerebral cortex Present Regionally restricted 10B Merge (high mag row 4) , 10B ACSA-2 (high mag row 4) The plaques in App<NL-G-F> cortex exclude astrocytes, and are devoid of staining. Astrocytes immediately adjacent to the plaques show immunoreactivity.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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