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Gene Expression Data
Assay Details
Assay
Reference: J:279927 Batiuk MY, et al., An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody. J Biol Chem. 2017 May 26;292(21):8874-8891
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7619788
Gene symbol: Slc1a2
Gene name: solute carrier family 1 (glial high affinity glutamate transporter), member 2
Antibody: Anti-GLT-1 (AB1783)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 9A top: postnatal day 21 (more )
Note: Double labeled: green - Slc1a2; magenta - Atp1b2 (assay MGI:7619762).
Structure Cell Type Level Pattern Image Note
TS28: visual cortex astrocyte of the cerebral cortex Present 9A GLT-1 , 9A Merge top Astrocyte staining pattern was highly similar to Atp1b2 immunostaining although non-overlapping.
Specimen 9B bottom: postnatal day 21 (more )
Note: Double labeled: green - Slc1a2; magenta - Atp1b2 (assay MGI:7619762).
Structure Cell Type Level Pattern Image Note
TS28: corpus callosum astrocyte of the cerebral cortex Present 9B Merge bottom , 9B GLT-1 Astrocyte Atp1b2 immunostaining and Slc1a2 immunostaining colocalize to the same cell, albeit in non-overlapping domains.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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