Gene Expression Data
Assay Details
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Assay
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Reference:
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J:270646
Limi S, et al., Transcriptional burst fraction and size dynamics during lens fiber cell differentiation and detailed insights into the denucleation process. J Biol Chem. 2018 Aug 24;293(34):13176-13190
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7618443
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Gene symbol:
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Polr2a
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Gene name:
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polymerase (RNA) II (DNA directed) polypeptide A
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Results
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Specimen
9 region a WT:
postnatal day 1
(more  )
Note: This region represents the least differentiated lens tissue.
Specimen
9 region a WT:
(close  )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This region represents the least differentiated lens tissue.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
9A a WT
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There is punctate staining in lens fiber cells that include regions a, b, and c.
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Specimen
9 region b WT:
postnatal day 1
(more )
Note: This region represents the intermediate differentiated lens tissue.
Specimen
9 region b WT:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This region represents the intermediate differentiated lens tissue.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
9A b WT
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There is punctate staining in lens fiber cells that include regions a, b, and c.
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Specimen
9 region c WT:
postnatal day 1
(more )
Note: This region represents the most highly differentiated lens tissue.
Specimen
9 region c WT:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This region represents the most highly differentiated lens tissue.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
9A c WT
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There is punctate staining in lens fiber cells that include regions a, b, and c.
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Specimen
9 region a Snf2hnull:
postnatal day 1; Smarca5tm2.1Ais/ Smarca5tm2.1Ais
(more )
Note: This region represents the least differentiated lens tissue.
Specimen
9 region a Snf2hnull:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J * SJL
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Mutant Allele(s):
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Smarca5tm2.1Ais/Smarca5tm2.1Ais
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This region represents the least differentiated lens tissue.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
9A a Snf2hnull
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Expression is altered due to disorganized lens fibers in this mutant.
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Specimen
9 region b Snf2hnull:
postnatal day 1; Smarca5tm2.1Ais/ Smarca5tm2.1Ais
(more )
Note: This region represents the intermediate differentiated lens tissue.
Specimen
9 region b Snf2hnull:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J * SJL
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Mutant Allele(s):
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Smarca5tm2.1Ais/Smarca5tm2.1Ais
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
|
This region represents the intermediate differentiated lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
9A b Snf2hnull
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Expression is altered due to disorganized lens fibers in this mutant.
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Specimen
9 region c Snf2hnull:
postnatal day 1; Smarca5tm2.1Ais/ Smarca5tm2.1Ais
(more )
Note: This region represents the most highly differentiated lens tissue.
Specimen
9 region c Snf2hnull:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J * SJL
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Mutant Allele(s):
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Smarca5tm2.1Ais/Smarca5tm2.1Ais
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
|
This region represents the most highly differentiated lens tissue.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
9A c Snf2hnull
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Expression is altered due to disorganized lens fibers in this mutant.
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Specimen
S3a:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - beta-actin.
Specimen
S3a:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Polr2a; red - beta-actin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S3a Pol II
,
S3a Merge
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Expression is in transcription start sites in the nuclei.
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Specimen
S3b:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - beta-actin.
Specimen
S3b:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Polr2a; red - beta-actin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S3b Merge
,
S3b Pol II
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Expression is in transcription start sites in the nuclei.
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Specimen
S3c:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - beta-actin.
Specimen
S3c:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
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Note:
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Double labeled: green - Polr2a; red - beta-actin.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S3c Pol II
,
S3c Merge
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Expression is in transcription start sites in the nuclei.
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Specimen
S4a:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
Specimen
S4a:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S4a Pol II
,
S4a Merge
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Expression is in transcription start sites in the nuclei.
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Specimen
S4b:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
Specimen
S4b:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S4b Merge
,
S4b Pol II
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Expression is in transcription start sites in the nuclei.
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Specimen
S4c:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
Specimen
S4c:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S4c Merge
,
S4c Pol II
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Expression is in transcription start sites in the nuclei.
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Specimen
S5a:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
Specimen
S5a:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
|
Cryosection
|
|
Note:
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Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S5a Merge
,
S5a Pol II
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Expression is in transcription start sites in the nuclei.
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Specimen
S5b:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
Specimen
S5b:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S5b Pol II
,
S5b Merge
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Expression is in transcription start sites in the nuclei.
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Specimen
S5c:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
Specimen
S5c:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
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Note:
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Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S5c Merge
,
S5c Pol II
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Expression is in transcription start sites in the nuclei.
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Specimen
S6a:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
Specimen
S6a:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
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Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S6a Pol II
,
S6a Merge
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Expression is in transcription start sites in the nuclei.
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Specimen
S6b:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
Specimen
S6b:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
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Cryosection
|
|
Note:
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Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S6b Pol II
,
S6b Merge
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Expression is in transcription start sites in the nuclei.
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Specimen
S6c:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
Specimen
S6c:
(close )
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Genetic Background:
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FVB
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S6c Merge
,
S6c Pol II
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Expression is in transcription start sites in the nuclei.
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Analysis Tools