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Gene Expression Data
Assay Details
Assay
Reference: J:270646 Limi S, et al., Transcriptional burst fraction and size dynamics during lens fiber cell differentiation and detailed insights into the denucleation process. J Biol Chem. 2018 Aug 24;293(34):13176-13190
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7618443
Gene symbol: Polr2a
Gene name: polymerase (RNA) II (DNA directed) polypeptide A
Antibody: anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) (ab5131)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 9 region a WT: postnatal day 1 (more )
Note: This region represents the least differentiated lens tissue.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 9A a WT There is punctate staining in lens fiber cells that include regions a, b, and c.
Specimen 9 region b WT: postnatal day 1 (more )
Note: This region represents the intermediate differentiated lens tissue.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 9A b WT There is punctate staining in lens fiber cells that include regions a, b, and c.
Specimen 9 region c WT: postnatal day 1 (more )
Note: This region represents the most highly differentiated lens tissue.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 9A c WT There is punctate staining in lens fiber cells that include regions a, b, and c.
Specimen 9 region a Snf2hnull: postnatal day 1; Smarca5tm2.1Ais/Smarca5tm2.1Ais (more )
Note: This region represents the least differentiated lens tissue.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 9A a Snf2hnull Expression is altered due to disorganized lens fibers in this mutant.
Specimen 9 region b Snf2hnull: postnatal day 1; Smarca5tm2.1Ais/Smarca5tm2.1Ais (more )
Note: This region represents the intermediate differentiated lens tissue.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 9A b Snf2hnull Expression is altered due to disorganized lens fibers in this mutant.
Specimen 9 region c Snf2hnull: postnatal day 1; Smarca5tm2.1Ais/Smarca5tm2.1Ais (more )
Note: This region represents the most highly differentiated lens tissue.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 9A c Snf2hnull Expression is altered due to disorganized lens fibers in this mutant.
Specimen S3a: postnatal day 1 (more )
Note: Double labeled: green - Polr2a; red - beta-actin.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S3a Pol II , S3a Merge Expression is in transcription start sites in the nuclei.
Specimen S3b: postnatal day 1 (more )
Note: Double labeled: green - Polr2a; red - beta-actin.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S3b Merge , S3b Pol II Expression is in transcription start sites in the nuclei.
Specimen S3c: postnatal day 1 (more )
Note: Double labeled: green - Polr2a; red - beta-actin.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S3c Pol II , S3c Merge Expression is in transcription start sites in the nuclei.
Specimen S4a: postnatal day 1 (more )
Note: Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S4a Pol II , S4a Merge Expression is in transcription start sites in the nuclei.
Specimen S4b: postnatal day 1 (more )
Note: Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S4b Merge , S4b Pol II Expression is in transcription start sites in the nuclei.
Specimen S4c: postnatal day 1 (more )
Note: Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S4c Merge , S4c Pol II Expression is in transcription start sites in the nuclei.
Specimen S5a: postnatal day 1 (more )
Note: Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S5a Merge , S5a Pol II Expression is in transcription start sites in the nuclei.
Specimen S5b: postnatal day 1 (more )
Note: Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S5b Pol II , S5b Merge Expression is in transcription start sites in the nuclei.
Specimen S5c: postnatal day 1 (more )
Note: Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S5c Merge , S5c Pol II Expression is in transcription start sites in the nuclei.
Specimen S6a: postnatal day 1 (more )
Note: Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S6a Pol II , S6a Merge Expression is in transcription start sites in the nuclei.
Specimen S6b: postnatal day 1 (more )
Note: Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S6b Pol II , S6b Merge Expression is in transcription start sites in the nuclei.
Specimen S6c: postnatal day 1 (more )
Note: Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S6c Merge , S6c Pol II Expression is in transcription start sites in the nuclei.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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07/29/2025
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