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Gene Expression Data
Assay Details
Assay
Reference: J:270646 Limi S, et al., Transcriptional burst fraction and size dynamics during lens fiber cell differentiation and detailed insights into the denucleation process. J Biol Chem. 2018 Aug 24;293(34):13176-13190
Assay type: RNA in situ
MGI Accession ID: MGI:7618450
Gene symbol: Cryga
Gene name: crystallin, gamma A
Probe: gammaA-Crystallin exon oligo
Probe preparation: Antisense RNA
Visualized with: Fluorescence
Assay notes: Probes were detected by CAL Fluor Red 610 and Quasar 670.
Results
Specimen 2 region a: postnatal day 1 (more )
Note: This region represents the least differentiated lens tissue.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 2_region a gammaA Expression is in lens fiber cells as one or two transcription sites per individual nuclei.
Specimen 2 region b: postnatal day 1 (more )
Note: This region represents the intermediate differentiated lens tissue.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 2_region b gammaA Expression is in lens fiber cells as one or two transcription sites per individual nuclei.
Specimen 2 region c: postnatal day 1 (more )
Note: This region represents the most highly differentiated lens tissue.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 2_region c gammaA Expression is in lens fiber cells as one or two transcription sites per individual nuclei.
Specimen 2 region d: postnatal day 1 (more )
Note: This region contained degraded pyknotic nuclear remnants with no intact nuclei.
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 2_region d gammaA Expression is in lens fiber cells as one or two transcription sites per individual nuclei.
Specimen 4 region a: embryonic day 14.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS22: lens fiber secondary lens fiber Present Regionally restricted 4_region a gammaA Expression is in early differentiating secondary lens fiber cells.
Specimen 4 region b: embryonic day 14.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS22: lens fiber secondary lens fiber Present Regionally restricted 4_region b gammaA Expression is in early differentiating secondary lens fiber cells.
Specimen 4 region c and d: embryonic day 14.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS22: lens fiber secondary lens fiber Present Regionally restricted 4_region c and dgammaA Expression is in early differentiating secondary lens fiber cells.
Specimen 5 region a: embryonic day 16.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS24: lens fiber lens fiber cell Present Punctate 5_region a gammaA Expression is in lens fiber cells as transcription sites per individual nuclei.
Specimen 5 region b: embryonic day 16.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS24: lens fiber lens fiber cell Present Punctate 5_region b gammaA Expression is in lens fiber cells as transcription sites per individual nuclei.
Specimen 5 region c: embryonic day 16.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS24: lens fiber lens fiber cell Present Punctate 5_region c gammaA Expression is in lens fiber cells as transcription sites per individual nuclei.
Specimen 5 region d: embryonic day 16.5 (more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Structure Cell Type Level Pattern Image Note
TS24: lens fiber lens fiber cell Present Punctate 5_region d gammaA Expression is in lens fiber cells as transcription sites per individual nuclei.
Specimen 9B WT: postnatal day 1 (more )
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 9B gammaA WT
Specimen 9B Snf2hnull: postnatal day 1; Smarca5tm2.1Ais/Smarca5tm2.1Ais (more )
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate 9B gammaA Snf2hnull
Specimen S6 region a: postnatal day 1 (more )
Note: Double labeled: red - Cryga; green - Polr2a (assay MGI:7618443).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S6a Merge , S6a gammaA Expression is in lens fiber cells as one or two transcription sites per individual nuclei.
Specimen S6 region b: postnatal day 1 (more )
Note: Double labeled: red - Cryga; green - Polr2a (assay MGI:7618443).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S6b gammaA , S6b Merge Expression is in lens fiber cells as one or two transcription sites per individual nuclei.
Specimen S6 region c: postnatal day 1 (more )
Note: Double labeled: red - Cryga; green - Polr2a (assay MGI:7618443).
Structure Cell Type Level Pattern Image Note
TS27: lens fiber lens fiber cell Present Punctate S6c Merge , S6c gammaA Expression is in lens fiber cells as one or two transcription sites per individual nuclei.
Specimen S7: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: lens epithelium Absent S7_gammaA
Specimen not shown: embryonic day 12.5 (more )
Structure Cell Type Level Pattern Image Note
TS20: lens primordium primary lens fiber Trace
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory