|
Results
|
Specimen
2 region a:
postnatal day 1
(more  )
Note: This region represents the least differentiated lens tissue.
Specimen
2 region a:
(close  )
|
Genetic Background:
|
FVB
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
This region represents the least differentiated lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Regionally restricted |
2_region a alphaA
|
Expression is in early differentiating lens fiber cells as one or two transcription sites per individual nuclei and is absent in the area where denucleation has occurred.
|
Specimen
2 region b:
postnatal day 1
(more )
Note: This region represents the intermediate differentiated lens tissue.
Specimen
2 region b:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
This region represents the intermediate differentiated lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Regionally restricted |
2_region b alphaA
|
Expression is in early differentiating lens fiber cells as one or two transcription sites per individual nuclei and is absent in the area where denucleation has occurred.
|
Specimen
2 region c:
postnatal day 1
(more )
Note: This region represents the most highly differentiated lens tissue.
Specimen
2 region c:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
This region represents the most highly differentiated lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Regionally restricted |
2_region c alphaA
|
Expression is as one or two transcription sites per individual nuclei and is reduced during lens fiber differentiation and is absent in the area where denucleation has occurred.
|
Specimen
2 region d:
postnatal day 1
(more )
Note: This region contained degraded pyknotic nuclear remnants with no intact nuclei.
Specimen
2 region d:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
This region contained degraded pyknotic nuclear remnants with no intact nuclei.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Regionally restricted |
2_region d alphaA
|
Expression is in early differentiating lens fiber cells and is absent in the area where denucleation has occurred (shown).
|
Specimen
3 region a:
embryonic day 12.5
(more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Specimen
3 region a:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: lens primordium |
primary lens fiber |
Present |
Regionally restricted |
3_region a alphaA
|
Expression is in the early differentiating primary lens fibers.
|
Specimen
3 region b:
embryonic day 12.5
(more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Specimen
3 region b:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: lens primordium |
primary lens fiber |
Present |
Regionally restricted |
3_region b alphaA
|
Expression is in the early differentiating primary lens fibers.
|
Specimen
3 region c and d:
embryonic day 12.5
(more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Specimen
3 region c and d:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
|
Specimen
4 region a:
embryonic day 14.5
(more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Specimen
4 region a:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: lens fiber |
secondary lens fiber |
Present |
Regionally restricted |
4_region a alphaA
|
Expression is in early differentiating secondary lens fiber cells.
|
Specimen
4 region b:
embryonic day 14.5
(more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Specimen
4 region b:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: lens fiber |
secondary lens fiber |
Present |
Regionally restricted |
4_region b alphaA
|
Expression is in early differentiating secondary lens fiber cells.
|
Specimen
4 region c and d:
embryonic day 14.5
(more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Specimen
4 region c and d:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: lens fiber |
secondary lens fiber |
Present |
Regionally restricted |
4_region c and d alphaA
|
Expression is in early differentiating secondary lens fiber cells.
|
Specimen
5 region a:
embryonic day 16.5
(more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Specimen
5 region a:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: lens fiber |
lens fiber cell |
Present |
Punctate |
5_region a alphaA
|
Expression is in lens fiber cells as transcription sites per individual nuclei.
|
Specimen
5 region b:
embryonic day 16.5
(more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Specimen
5 region b:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: lens fiber |
lens fiber cell |
Present |
Punctate |
5_region b alphaA
|
Expression is in lens fiber cells as transcription sites per individual nuclei.
|
Specimen
5 region c:
embryonic day 16.5
(more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Specimen
5 region c:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: lens fiber |
lens fiber cell |
Present |
Punctate |
5_region c alphaA
|
Expression is in lens fiber cells as transcription sites per individual nuclei.
|
Specimen
5 region d:
embryonic day 16.5
(more )
Note: Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
Specimen
5 region d:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Regions a, b, c, and d indicate progressive stages of differentiation throughout the lens tissue.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: lens fiber |
lens fiber cell |
Present |
Punctate |
5_region d alphaA
|
Expression is in lens fiber cells as transcription sites per individual nuclei.
|
Specimen
9B WT:
postnatal day 1
(more )
Specimen
9B WT:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
9B Snf2hnull:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6J * SJL
|
|
Mutant Allele(s):
|
Smarca5tm2.1Ais/Smarca5tm2.1Ais
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S2:
postnatal day 1
(more )
Note: Double labeled: red - Cryaa mRNA; green - Cryaa mRNA (assay MGI:7618445).
Specimen
S2:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Cryaa mRNA; green - Cryaa mRNA (assay MGI:7618445).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Regionally restricted |
S2_alphaA intron/exon
,
S2_alphaA exon
|
Expression is in early differentiating lens fiber cells as one or two transcription sites per individual nuclei and is absent in the area where denucleation has occurred.
|
Specimen
S4 region a:
postnatal day 1
(more )
Note: Double labeled: red - Cryaa; green - Polr2a (assay MGI:7618443).
Specimen
S4 region a:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Cryaa; green - Polr2a (assay MGI:7618443).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Regionally restricted |
S4a Merge
,
S4a alphaA
|
Expression is in early differentiating lens fiber cells as one or two transcription sites per individual nuclei and is absent in the area where denucleation has occurred.
|
Specimen
S4 region b:
postnatal day 1
(more )
Note: Double labeled: red - Cryaa; green - Polr2a (assay MGI:7618443).
Specimen
S4 region b:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Cryaa; green - Polr2a (assay MGI:7618443).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Regionally restricted |
S4b Merge
,
S4b alphaA
|
Expression is in early differentiating lens fiber cells as one or two transcription sites per individual nuclei and is absent in the area where denucleation has occurred.
|
Specimen
S4 region c:
postnatal day 1
(more )
Note: Double labeled: red - Cryaa; green - Polr2a (assay MGI:7618443).
Specimen
S4 region c:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Cryaa; green - Polr2a (assay MGI:7618443).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: lens fiber |
lens fiber cell |
Present |
Regionally restricted |
S4c Merge
,
S4c AlphaA
|
Expression is in early differentiating lens fiber cells as one or two transcription sites per individual nuclei and is absent in the area where denucleation has occurred.
|
Specimen
S7:
postnatal day 1
(more )
Specimen
S7:
(close )
|
Genetic Background:
|
FVB
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Analysis Tools