About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:178497 Huettl RE, et al., Cranial nerve fasciculation and Schwann cell migration are impaired after loss of Npn-1. Dev Biol. 2011 Nov 15;359(2):230-41
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7514320
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Results
Specimen 1B: embryonic day 10.5 (more )
Note: Control littermates were Npn-1cond+/+;Cre+ or Npn-1cond-/-;Cre-.
Structure Level Pattern Image Note
TS18: cranial nerve Present Widespread 1B
Specimen 1C: embryonic day 10.5 (more )
Note: Control littermates were Npn-1cond+/+;Cre+ or Npn-1cond-/-;Cre-.
Structure Level Pattern Image Note
TS18: glossopharyngeal nerve Present 1C
TS18: cranial nerve Present 1C Immunostaining of cranial projections including developing vagus projections.
Specimen 1D: embryonic day 10.5; Nrp1tm2Ddg/Nrp1tm2Ddg, Tg(PLAT-cre)116Sdu/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: cranial nerve Present Widespread 1D Mutation led to phenotypic defects including distal defasciculation of the facial nerve, and the ophthalmic, maxillary and mandibular branches of the trigeminal ganglion. Ectopic projections between the facial and trigeminal ganglia were also observed.
Specimen 1E: embryonic day 10.5; Nrp1tm2Ddg/Nrp1tm2Ddg, Tg(PLAT-cre)116Sdu/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: glossopharyngeal nerve Present 1E Mutation led to phenotypic defects including distal defasciculation.
TS18: cranial nerve Present 1E Mutation led to phenotypic defects including distal defasciculation of the glossopharyngeal and vagus projections.
Specimen 1F: embryonic day 11.5 (more )
Note: Control littermates were Npn-1cond+/+;Cre+ or Npn-1cond-/-;Cre-.
Structure Level Pattern Image Note
TS20: cranial nerve Present 1F
TS20: trigeminal nerve mandibular division Present 1F
TS20: trigeminal nerve maxillary division Present 1F
TS20: trigeminal nerve ophthalmic division Present 1F
Specimen 1G: embryonic day 11.5 (more )
Note: Control littermates were Npn-1cond+/+;Cre+ or Npn-1cond-/-;Cre-.
Structure Level Pattern Image Note
TS20: cranial nerve Present 1G
TS20: facial nerve Present 1G
TS20: glossopharyngeal nerve Present 1G
TS20: vagus nerve Present 1G
Specimen 1H: embryonic day 11.5; Nrp1tm2Ddg/Nrp1tm2Ddg, Tg(PLAT-cre)116Sdu/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: cranial nerve Present 1H Mutation led to phenotypic defects including distal defasciculation. Ectopic projections were also observed between facial and trigeminal ganglia.
TS20: trigeminal nerve mandibular division Present 1H Mutation led to phenotypic defects including distal defasciculation.
TS20: trigeminal nerve maxillary division Present 1H Mutation led to phenotypic defects including distal defasciculation.
TS20: trigeminal nerve ophthalmic division Present 1H Mutation led to phenotypic defects including distal defasciculation.
Specimen 1I: embryonic day 11.5; Nrp1tm2Ddg/Nrp1tm2Ddg, Tg(PLAT-cre)116Sdu/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: cranial nerve Present 1I Mutation led to phenotypic defects including distal defasciculation.
TS20: facial nerve Present 1I Mutation led to phenotypic defects including distal defasciculation.
TS20: glossopharyngeal nerve Present 1I Mutation led to phenotypic defects including distal defasciculation.
TS20: vagus nerve Present 1I Mutation led to phenotypic defects including distal defasciculation.
Specimen 5B: embryonic day 11.5 (more )
Note: Control littermates were Npn-1cond+/+;Cre+ or Npn-1cond-/-;Cre-. Double labeled: green - Nefm; red - Sox10 (assay MGI:7514336).
Structure Level Pattern Image Note
TS20: hypoglossal nerve Present 5B
Specimen 5C: embryonic day 11.5; Nrp1tm2Ddg/Nrp1tm2Ddg, Tg(PLAT-cre)116Sdu/0 (more )
Note: Conditional mutant. Double labeled: green - Nefm; red - Sox10 (assay MGI:7514336).
Structure Level Pattern Image Note
TS20: hypoglossal nerve Present 5C Defective fasciculation was noted.
Specimen 5D: embryonic day 11.5; Gt(ROSA)26Sortm1(DTA)Riet/Gt(ROSA)26Sortm1(DTA)Riet, Tg(PLAT-cre)116Sdu/0 (more )
Note: Conditional mutant. Double labeled: green - Nefm; red - Sox10 (assay MGI:7514336).
Structure Level Pattern Image Note
TS20: hypoglossal nerve Present 5D Defective initial assembly was noted.
Specimen 5E: embryonic day 11.5 (more )
Note: Control littermates were Npn-1cond+/+;Cre+ or Npn-1cond-/-;Cre-. Double labeled: green - Nefm; red - Sox10 (assay MGI:7514336).
Structure Level Pattern Image Note
TS20: hypoglossal nerve Present 5E
Specimen 5F: embryonic day 11.5; Nrp1tm2Ddg/Nrp1tm2Ddg, Tg(PLAT-cre)116Sdu/0 (more )
Note: Conditional mutant. Double labeled: green - Nefm; red - Sox10 (assay MGI:7514336).
Structure Level Pattern Image Note
TS20: hypoglossal nerve Present 5F
Specimen 5G: embryonic day 11.5; Gt(ROSA)26Sortm1(DTA)Riet/Gt(ROSA)26Sortm1(DTA)Riet, Tg(PLAT-cre)116Sdu/0 (more )
Note: Conditional mutant. Double labeled: green - Nefm; red - Sox10 (assay MGI:7514336).
Structure Level Pattern Image Note
TS20: hypoglossal nerve Present 5G
Specimen S1D: embryonic day 10.5 (more )
Note: Control littermates were Npn-1cond+/+;Cre+ or Npn-1cond-/-;Cre-. Double labeled: green - Nefm; red - Emcn (assay MGI:7514350).
Structure Level Pattern Image Note
TS18: cranial nerve Present S1D Expression was at the level of the hypoglossal nerve.
Specimen S1E: embryonic day 10.5; Nrp1tm2Ddg/Nrp1tm2Ddg, Tg(PLAT-cre)116Sdu/0 (more )
Note: Conditional mutant. Double labeled: green - Nefm; red - Emcn (assay MGI:7514350).
Structure Level Pattern Image Note
TS18: cranial nerve Present Regionally restricted S1E Expression was at the level of the hypoglossal nerve.
Specimen S2B: embryonic day 11.5 (more )
Note: Control littermates were Npn-1cond+/+;Cre+ or Npn-1cond-/-;Cre-.
Structure Level Pattern Image Note
TS20: facial nerve Present S2B
TS20: trigeminal nerve Present S2B
Specimen S2C: embryonic day 11.5 (more )
Note: Control littermates were Npn-1cond+/+;Cre+ or Npn-1cond-/-;Cre-.
Structure Level Pattern Image Note
TS20: facial nerve Present S2C
TS20: glossopharyngeal nerve Present S2C
TS20: vagus nerve Present S2C
Specimen S2D: embryonic day 11.5; Nrp1tm1Ddg/Nrp1tm1Ddg (more )
Structure Level Pattern Image Note
TS20: facial nerve Present S2D Projections of nerves were defasciculated in the periphery in the mutant embryos.
TS20: trigeminal nerve Present S2D Projections of nerves were defasciculated in the periphery in the mutant embryos.
TS20: cranial nerve Present Regionally restricted S2D Aberrant projections between trigeminal and facial ganglia were observed in mutant embryos.
Specimen S2E: embryonic day 11.5; Nrp1tm1Ddg/Nrp1tm1Ddg (more )
Structure Level Pattern Image Note
TS20: facial nerve Present S2E Projections of nerves were defasciculated in the periphery in the mutant embryos.
TS20: glossopharyngeal nerve Present S2E Projections of nerves were defasciculated in the periphery in the mutant embryos.
TS20: vagus nerve Present S2E Projections of nerves were defasciculated in the periphery in the mutant embryos.
Specimen S2F: embryonic day 11.5 (more )
Note: Control littermates were Npn-1cond+/+;Cre+ or Npn-1cond-/-;Cre-. Double labeled: green - Nefm; red - Sox10 (assay MGI:7514336).
Structure Level Pattern Image Note
TS20: hypoglossal nerve Present S2F
Specimen S2G: embryonic day 11.5; Nrp1tm1Ddg/Nrp1tm1Ddg (more )
Note: Double labeled: green - Nefm; red - Sox10 (assay MGI:7514336).
Structure Level Pattern Image Note
TS20: hypoglossal nerve Present S2G
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/14/2024
MGI 6.23 		The Jackson Laboratory