Nrp1^tm1Ddg
Targeted Allele Detail

Summary

• Symbol: Nrp1^tm1Ddg
• Name: neuropilin 1; targeted mutation 1, David D Ginty
• MGI ID: MGI:3512099
• Synonyms: npn-1^Sema-, Nrp1^sema
• Gene: Nrp1 Location: Chr8:129085085-129229844 bp, + strand Genetic Position: Chr8, 75.78 cM, cytoband E
• Alliance: Nrp1^tm1Ddg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93799
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Exon 2 was replaced with a mutated exon 2 encoding 7 amino acid substitutions (H46E, S47A, S51A, E52R, K53D, D79K, R80E) in three regions of the amino-terminal CUB domain, and a loxP site flanked neomycin resistance gene cassette was inserted into intron 1. The neo cassette was removed through subsequent cre-mediated recombination. Mutation of these sites was shown to abolish Sema3 family binding but not VEGF binding. Immunohistochemistry showed that homozygotes express normal levels of protein. (J:93799)

Expression

In Mice Carrying this Mutation:	46 assay results
In Structures Affected by this Mutation:	11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Nrp1 Mutation: 81 strains or lines available

References

• Original: J:93799 Gu C, et al., Neuropilin-1 conveys semaphorin and VEGF signaling during neural and cardiovascular development. Dev Cell. 2003 Jul;5(1):45-57
• All: 37 reference(s)