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Gene Expression Data
Assay Details
Assay
Reference: J:305946 Xu M, et al., The SRCAP chromatin remodeling complex promotes oxidative metabolism during prenatal heart development. Development. 2021 Apr 15;148(8):dev199026
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7448838
Gene symbol: Tnnt2
Gene name: troponin T2, cardiac
Results
Specimen 3F LV Ctrl: embryonic day 12.5 (more )
Note: Double labeled: green - Tnnt2; white - Pecam1 (assay MGI:7448839).
Structure Level Pattern Image Note
TS20: heart left ventricle Present 3F Ctrl LV right
Specimen 3F LV KO: embryonic day 12.5; Znhit1tm1.1Nju/Znhit1tm1.1Nju, Tg(Tnnt2-rtTA,tetO-cre)1Wtp/0 (more )
Note: Conditional mutant. Double labeled: green - Tnnt2; white - Pecam1 (assay MGI:7448839).
Structure Level Pattern Image Note
TS20: heart left ventricle Present 3F KO LV right
Specimen 3F RV Ctrl: embryonic day 12.5 (more )
Note: Double labeled: green - Tnnt2; white - Pecam1 (assay MGI:7448839).
Structure Level Pattern Image Note
TS20: heart right ventricle Present 3F Ctrl RV high mag , 3F Ctrl RV right
Specimen 3F RV KO: embryonic day 12.5; Znhit1tm1.1Nju/Znhit1tm1.1Nju, Tg(Tnnt2-rtTA,tetO-cre)1Wtp/0 (more )
Note: Conditional mutant. Double labeled: green - Tnnt2; white - Pecam1 (assay MGI:7448839).
Structure Level Pattern Image Note
TS20: heart right ventricle Present 3F KO RV high mag , 3F KO RV right
Specimen S3A top: embryonic day 10.5 (more )
Note: Triple labeled: green - Tnnt2; white - Pecam1 (assay MGI:7448839); red - Znhit1 (assay MGI:7448864).
Structure Level Pattern Image Note
TS17: heart Present S3A top
Specimen S3A bottom: embryonic day 11.5 (more )
Note: Triple labeled: green - Tnnt2; white - Pecam1 (assay MGI:7448839); red - Znhit1 (assay MGI:7448864).
Structure Level Pattern Image Note
TS19: heart Present S3A bottom
Specimen S4A Ctrl: embryonic day 10.5 (more )
Note: Double labeled: red - Tnnt2; green - Mki67 (assay MGI:7448888).
Structure Cell Type Level Pattern Image Note
TS17: cardiac muscle tissue cardiac muscle cell Present Regionally restricted S4A top Co-expression of Mki67 and Tnnt2 was detected in proliferating cardiomyocytes.
Specimen S4A KO: embryonic day 10.5; Znhit1tm1.1Nju/Znhit1tm1.1Nju, Tg(Tnnt2-rtTA,tetO-cre)1Wtp/0 (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - Mki67 (assay MGI:7448888).
Structure Cell Type Level Pattern Image Note
TS17: cardiac muscle tissue cardiac muscle cell Present Regionally restricted S4A bottom Quantification of proliferating cardiomyocytes was not significantly different in the mutant compared to control at E10.5.
Specimen S4B Ctrl E11.5: embryonic day 11.5 (more )
Note: Double labeled: red - Tnnt2; green - Mki67 (assay MGI:7448888).
Structure Cell Type Level Pattern Image Note
TS19: cardiac muscle tissue cardiac muscle cell Present Regionally restricted S4B Ctrl E11.5 Co-expression of Mki67 and Tnnt2 was detected in proliferating cardiomyocytes.
Specimen S4B KO E11.5: embryonic day 11.5; Znhit1tm1.1Nju/Znhit1tm1.1Nju, Tg(Tnnt2-rtTA,tetO-cre)1Wtp/0 (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - Mki67 (assay MGI:7448888).
Structure Cell Type Level Pattern Image Note
TS19: cardiac muscle tissue cardiac muscle cell Present Regionally restricted S4B KO E11.5 Quantification of proliferating cardiomyocytes was not significantly different in the mutant compared to control at E11.5.
Specimen S4B Ctrl E12.5: embryonic day 12.5 (more )
Note: Double labeled: red - Tnnt2; green - Mki67 (assay MGI:7448888).
Structure Cell Type Level Pattern Image Note
TS20: cardiac muscle tissue cardiac muscle cell Present Regionally restricted S4B Ctrl E12.5 Co-expression of Mki67 and Tnnt2 was detected in proliferating cardiomyocytes.
Specimen S4B KO E12.5: embryonic day 12.5; Znhit1tm1.1Nju/Znhit1tm1.1Nju, Tg(Tnnt2-rtTA,tetO-cre)1Wtp/0 (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - Mki67 (assay MGI:7448888).
Structure Cell Type Level Pattern Image Note
TS20: cardiac muscle tissue cardiac muscle cell Present Regionally restricted S4B KO E12.5 Double labeled cells indicated decreased cardiomyocyte proliferation after E12.5.
Specimen S4C Ctrl E11.5: embryonic day 11.5 (more )
Note: Double labeled: red - Tnnt2; green - pHH3.
Structure Cell Type Level Pattern Image Note
TS19: cardiac muscle tissue cardiac muscle cell Present Regionally restricted S4C Ctrl E11.5 Co-expression of Tnnt2 and pHH3 was detected in proliferating cardiomyocytes.
Specimen S4C KO E11.5: embryonic day 11.5; Znhit1tm1.1Nju/Znhit1tm1.1Nju, Tg(Tnnt2-rtTA,tetO-cre)1Wtp/0 (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - pHH3.
Structure Cell Type Level Pattern Image Note
TS19: cardiac muscle tissue cardiac muscle cell Present Regionally restricted S4C KO E11.5 Quantification of proliferating cardiomyocytes was not significantly different in the mutant compared to control at E11.5.
Specimen S4C Ctrl E12.5: embryonic day 12.5 (more )
Note: Double labeled: red - Tnnt2; green - pHH3.
Structure Cell Type Level Pattern Image Note
TS20: cardiac muscle tissue cardiac muscle cell Present Regionally restricted S4C Ctrl E12.5 Co-expression of Tnnt2 and pHH3 was detected in proliferating cardiomyocytes.
Specimen S4C KO E12.5: embryonic day 12.5; Znhit1tm1.1Nju/Znhit1tm1.1Nju, Tg(Tnnt2-rtTA,tetO-cre)1Wtp/0 (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - pHH3.
Structure Cell Type Level Pattern Image Note
TS20: cardiac muscle tissue cardiac muscle cell Present Regionally restricted S4C KO E12.5 Double labeled cells indicated decreased cardiomyocyte proliferation after E12.5.
Specimen S4C Ctrl E13.5: embryonic day 13.5 (more )
Note: Double labeled: red - Tnnt2; green - pHH3.
Structure Cell Type Level Pattern Image Note
TS21: cardiac muscle tissue cardiac muscle cell Present Regionally restricted S4C Ctrl E13.5 Co-expression of Tnnt2 and pHH3 was detected in proliferating cardiomyocytes.
Specimen S4C KO E13.5: embryonic day 13.5; Znhit1tm1.1Nju/Znhit1tm1.1Nju, Tg(Tnnt2-rtTA,tetO-cre)1Wtp/0 (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - pHH3.
Structure Cell Type Level Pattern Image Note
TS21: cardiac muscle tissue cardiac muscle cell Present Regionally restricted S4C KO E13.5 Double labeled cells indicated decreased cardiomyocyte proliferation after E12.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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