Pecam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:305946 Xu M, et al., The SRCAP chromatin remodeling complex promotes oxidative metabolism during prenatal heart development. Development. 2021 Apr 15;148(8):dev199026
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7448839
Gene symbol:	Pecam1
Gene name:	platelet/endothelial cell adhesion molecule 1

Antibody:

MEC 13.3

Results

3F Ctrl LV left ©

3F Ctrl LV right ©

Specimen 3F LV Ctrl: embryonic day 12.5 (more

)
Note: Double labeled: white - Pecam1; green - Tnnt2 (assay MGI:7448838).

Structure	Level	Pattern	Image	Note
TS20: left ventricle endocardial lining	Present		3F Ctrl LV right , 3F Ctrl LV left

3F KO LV left ©

3F KO LV right ©

Specimen 3F LV KO: embryonic day 12.5; Znhit1^tm1.1Nju/Znhit1^tm1.1Nju, Tg(Tnnt2-rtTA,tetO-cre)1Wtp/0 (more

)
Note: Conditional mutant. Double labeled: white - Pecam1; green - Tnnt2 (assay MGI:7448838).

Structure	Level	Pattern	Image	Note
TS20: left ventricle endocardial lining	Present		3F KO LV left , 3F KO LV right

3F Ctrl RV high mag ©

3F Ctrl RV left ©

3F Ctrl RV right ©

Specimen 3F RV Ctrl: embryonic day 12.5 (more

)
Note: Double labeled: white - Pecam1; green - Tnnt2 (assay MGI:7448838).

Structure	Level	Pattern	Image	Note
TS20: right ventricle endocardial lining	Present		3F Ctrl RV right , 3F Ctrl RV high mag , 3F Ctrl RV left

3F KO RV high mag ©

3F KO RV left ©

3F KO RV right ©

Specimen 3F RV KO: embryonic day 12.5; Znhit1^tm1.1Nju/Znhit1^tm1.1Nju, Tg(Tnnt2-rtTA,tetO-cre)1Wtp/0 (more

)
Note: Conditional mutant. Double labeled: white - Pecam1; green - Tnnt2 (assay MGI:7448838).

Structure	Level	Pattern	Image	Note
TS20: right ventricle endocardial lining	Present		3F KO RV left , 3F KO RV right , 3F KO RV high mag

S3A top ©

Specimen S3A top: embryonic day 10.5 (more

)
Note: Triple labeled: white - Pecam1; green - Tnnt2 (assay MGI:7448838); red - Znhit1 (assay MGI:7448864).

Structure	Level	Pattern	Image	Note
TS17: endocardial lining	Present		S3A top

S3A bottom ©

Specimen S3A bottom: embryonic day 11.5 (more

)
Note: Triple labeled: white - Pecam1; green - Tnnt2 (assay MGI:7448838); red - Znhit1 (assay MGI:7448864).

Structure	Level	Pattern	Image	Note
TS19: endocardial lining	Present		S3A bottom

S5D top ©

Specimen S5D Ctrl: embryonic day 12.5 (more

)
Note: Double labeled: red - Pecam1; white - alpha-actinin.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: right ventricle endocardial lining	cardiac endothelial cell	Present		S5D top

S5D bottom ©

Specimen S5D Znhit1^f/f;Mef2c-AHF Cre: embryonic day 12.5; Znhit1^tm1.1Nju/Znhit1^tm1.1Nju, Tg(Mef2c-cre)2Blk/0 (more

)
Note: Conditional mutant. Double labeled: red - Pecam1; white - alpha-actinin.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: right ventricle endocardial lining	cardiac endothelial cell	Present		S5D bottom

Specimen S5E Ctrl: embryonic day 18.5 (more

)
Note: Triple labeled: red - Pecam1; green - Vim (assay MGI:7448920); white - alpha-actinin.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: right ventricle endocardial lining	cardiac endothelial cell	Present		S5E Ctrl right , S5E Ctrl center , S5E Ctrl left

Specimen S5E Znhit1^f/f;Mef2c-AHF Cre: embryonic day 18.5; Znhit1^tm1.1Nju/Znhit1^tm1.1Nju, Tg(Mef2c-cre)2Blk/0 (more

)
Note: Conditional mutant. Triple labeled: red - Pecam1; green - Vim (assay MGI:7448920); white - alpha-actinin.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: right ventricle endocardial lining	cardiac endothelial cell	Present		S5E Znhit1^f/f;Mef2c-AHF Cre left , S5E Znhit1^f/f;Mef2c-AHF Cre right , S5E Znhit1^f/f;Mef2c-AHF Cre center

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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