About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:326689 Phan TP, et al., Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells. Genes Dev. 2022 Jun 1;36(11-12):718-736
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7442648
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4 [EPR17929] (ab181557)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 4B E10.25 +/+: embryonic day 10.25 (more )
Structure Level Pattern Image Note
TS16: primordial germ cell of dorsal mesentery Present 4B E10.25 +/+
Specimen 4B E10.25 +/deltau: embryonic day 10.25; Plk4em1Ahol/Plk4+ (more )
Structure Level Pattern Image Note
TS16: primordial germ cell of dorsal mesentery Present 4B E10.25 +/deltau Similar as in the wild-type.
Specimen 4B E10.25 deltau/deltau: embryonic day 10.25; Plk4em1Ahol/Plk4em1Ahol (more )
Structure Level Pattern Image Note
TS16: primordial germ cell of dorsal mesentery Present 4B E10.25 deltau/deltau Similar as in the wild-type.
Specimen 4B E10.5 +/+: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4B E10.5 +/+
Specimen 4B E10.5 +/deltau: embryonic day 10.5; Plk4em1Ahol/Plk4+ (more )
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4B E10.5 +/deltau Similar as in the wild-type.
Specimen 4B E10.5 deltau/deltau: embryonic day 10.5; Plk4em1Ahol/Plk4em1Ahol (more )
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4B E10.5 deltau/deltau Similar as in the wild-type.
Specimen 4C +/+: embryonic day 10.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: red - Pou5f1; magenta - Tubg1 (assay MGI:7442562) and Tubg2 (assay MGI:7442686); green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4C +/+
Specimen 4C deltau/deltau: embryonic day 10.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: red - Pou5f1; magenta - Tubg1 (assay MGI:7442562) and Tubg2 (assay MGI:7442686); green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4C deltau/deltau Similar as in the wild-type. A small fraction had an increased number of centrin-GFP foci that were often observed in a "flower-like" pattern.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory