Gt(ROSA)26Sor^{tm1.1(EGFP/CETN1)Sev}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(EGFP/CETN1)Sev}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Sylvia Evans
• MGI ID: MGI:7442432
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(EGFP/CETN1)Sev} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:326689
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector containing a loxP-flanked PGK-NEO cassette and a triplicate SV40 polyA sequence, followed by an EGFP-Centrin 1 fusion cassette (cDNA of the human centrin 1 gene, CETN1, fused in frame, with EGFP at the N-terminus) with Bovine Growth Hormone polyA sequence, was inserted into the Gt(ROSA)26Sor locus. Cre-mediated recombination removed the stop cassette allowing for ubiquitous expression. (J:326689)

Expression

In Mice Carrying this Mutation:

51 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

References

• Original: J:326689 Phan TP, et al., Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells. Genes Dev. 2022 Jun 1;36(11-12):718-736
• All: 1 reference(s)