About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:326689 Phan TP, et al., Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells. Genes Dev. 2022 Jun 1;36(11-12):718-736
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7442562
Gene symbol: Tubg1
Gene name: tubulin, gamma 1
Antibody: anti-gamma-tubulin
Detection system: Secondary antibody coupled to Alexa Fluor 647
Assay notes: The specificity of the antibody/probe used was not detailed; both family members have been annotated.
Results
Specimen 2C +/+: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: intestine lamina propria plasma cell Present 2C +/+ Expressed in the pericentriolar material.
Specimen 2C +/deltau: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4+ (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: intestine lamina propria plasma cell Present 2C +/deltau Expressed in the pericentriolar material, similar as in the wild-type.
Specimen 2C deltau/deltau: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: intestine lamina propria plasma cell Present 2C deltau/deltau Expressed in the pericentriolar material, similar as in the wild-type.
Specimen 2D +/+: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: epidermis keratinocyte Present 2D +/+ Expressed in the pericentriolar material.
Specimen 2D +/deltau: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4+ (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: epidermis keratinocyte Present 2D +/deltau Expressed in the pericentriolar material, similar as in the wild-type.
Specimen 2D deltau/deltau: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: epidermis keratinocyte Present 2D deltau/deltau Expressed in the pericentriolar material, similar as in the wild-type.
Specimen 2E +/+: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: spleen splenocyte Present 2E +/+ Expressed in the pericentriolar material.
Specimen 2E +/deltau: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4+ (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: spleen splenocyte Present 2E +/deltau Expressed in the pericentriolar material, similar as in the wild-type.
Specimen 2E deltau/deltau: postnatal week 8-12; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Double labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Cell Type Level Pattern Image Note
TS28: spleen splenocyte Present 2E deltau/deltau Expressed in the pericentriolar material, similar as in the wild-type.
Specimen 4C +/+: embryonic day 10.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Pou5f1 (assay MGI:7442648).
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4C +/+
Specimen 4C deltau/deltau: embryonic day 10.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Pou5f1 (assay MGI:7442648).
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4C deltau/deltau Similar as in the wild-type. A small fraction had an increased number of centrin-GFP foci that were often observed in a "flower-like" pattern.
Specimen 4G +/+: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4G merge +/+
Specimen 4G +/deltau: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4+ (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4G merge +/deltau An increased number of centrin-GFP foci was seen.
Specimen 4G deltau/deltau: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4G merge deltau/deltau An increased number of centrin-GFP foci was seen.
Specimen 5K +/+: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 5K +/+ lower , 5K +/+ upper
Specimen 5K deltau/deltau: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 5K deltau/deltau upper , 5K deltau/deltau lower An increased number of centrin-GFP foci was seen.
Specimen 5K deltau/-: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em2.1Ahol (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 5K deltau/- lower , 5K deltau/- upper No longer display centriole amplification.
Specimen S4D +/+: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present S4D +/+ lower , S4D +/+ upper
Specimen S4D deltau/deltau: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: magenta - Tubg1 and Tubg2; green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev; red - Ddx4 (assay MGI:7442667).
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present S4D deltau/deltau lower , S4D deltau/deltau upper Some positive cells with supernumerary centrioles exhibited multipolar mitotic spindles, suggesting that these cells could generate aneuploid daughter cells with micronuclei or abnormal nuclear shape.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory