Plk4^em1Ahol
Endonuclease-mediated Allele Detail

Summary

• Symbol: Plk4^em1Ahol
• Name: polo like kinase 4; endonuclease-mediated mutation 1, Andrew Holland
• MGI ID: MGI:7316773
• Synonyms: Plk4^deltau
• Gene: Plk4 Location: Chr3:40754463-40771318 bp, + strand Genetic Position: Chr3, 19.54 cM
• Alliance: Plk4^em1Ahol page

Mutation origin

• Strain of Origin: (C57BL/6 x SJL)F2

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/cas9 genome editing uses two guide RNAs (uORF1 sgRNA: 5'- CCGGCGGGAATTTTTCAAAA-3' and uORF2 sgRNA: 5'- GCGTAGAGAAGGCGTCCTGA-3') and a single-stranded DNA repair template to insert the desired modification (ATG start codons of uORF1 and uORF2 were changed to ATA). (J:326689)

Expression

In Mice Carrying this Mutation:

48 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Plk4 Mutation: 38 strains or lines available

References

• Original: J:326689 Phan TP, et al., Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells. Genes Dev. 2022 Jun 1;36(11-12):718-736
• All: 2 reference(s)