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Gene Expression Data
Assay Details
Assay
Reference: J:229936 Cano E, et al., Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections. Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):656-61
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7431138
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: Secondary antibody
Results
Specimen 1H: embryonic day 14.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant. Triple labeled: red - Pecam1; green - Cre reporter; blue - Acta2 (assay MGI:7431145).
Structure Cell Type Level Pattern Image Note
TS22: coronary artery endothelial cell of coronary artery Present 1H
Specimen 1I: embryonic day 16.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Cre reporter
Structure Cell Type Level Pattern Image Note
TS24: coronary artery endothelial cell of coronary artery Present 1I
Specimen 1J: embryonic day 18.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant. Triple labeled: red - Pecam1; green - Cre reporter; blue - Acta2 (assay MGI:7431145).
Structure Cell Type Level Pattern Image Note
TS26: coronary artery endothelial cell of coronary artery Present 1J
Specimen 2C: embryonic day 11.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Double labeled: red - Pecam1; green - Wt1 reporter (assay MGI:7431230).
Structure Level Pattern Image Note
TS19: visceral pericardium Absent 2C No expression in epicardium or epicardial-derived cells.
TS19: endocardial lining Present 2C
Specimen 2D: embryonic day 12.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Double labeled: red - Pecam1; green - Wt1 reporter (assay MGI:7431230).
Structure Cell Type Level Pattern Image Note
TS20: coronary vascular plexus endothelial cell Present 2D Coexpressed with Wt1 in coronary vasculature.
Specimen 2E'/E": embryonic day 12.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Double labeled: red - Pecam1; green - Wt1 reporter (assay MGI:7431230).
Structure Cell Type Level Pattern Image Note
TS20: coronary vascular plexus endothelial cell Present 2E" , 2E' Coexpressed with Wt1 in subepicardial coronary vasculature.
Specimen 2F: embryonic day 13.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Triple labeled: red - Pecam1; green - Wt1 reporter (assay MGI:7431230); blue - Acta2 (assay MGI:7431145).
Structure Cell Type Level Pattern Image Note
TS21: coronary artery endothelial cell of coronary artery Present 2F Coexpressed with Wt1 in intramyocardial coronary vasculature.
TS21: endocardial lining Present 2F
TS21: visceral pericardium Present 2F
Specimen 2G: embryonic day 14.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Triple labeled: red - Pecam1; green - Wt1 reporter (assay MGI:7431230); blue - Acta2 (assay MGI:7431145).
Structure Cell Type Level Pattern Image Note
TS22: coronary artery endothelial cell of coronary artery Present 2G Coexpressed with Wt1 in intramyocardial coronary vasculature.
TS22: endocardial lining endocardial cell Present 2G
TS22: visceral pericardium Present 2G
Specimen 2H: embryonic day 18.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Double labeled: red - Pecam1; green - Wt1 reporter (assay MGI:7431230).
Structure Cell Type Level Pattern Image Note
TS26: coronary artery endothelial cell of coronary artery Present 2H
Specimen 2J: embryonic day 13.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Wt1-cre)#Jbeb/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Cre reporter
Structure Level Pattern Image Note
TS21: coronary vascular plexus Present 2J
Specimen 2K: embryonic day 15.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Wt1-cre)#Jbeb/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Cre reporter
Structure Level Pattern Image Note
TS23: coronary vascular plexus Present 2K
Specimen 2L: embryonic day 18.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Wt1-cre)#Jbeb/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Cre reporter
Structure Cell Type Level Pattern Image Note
TS26: coronary artery endothelial cell of coronary artery Present 2L
Specimen 2N: postnatal newborn; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Wt1-cre)#Jbeb/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Cre reporter
Structure Cell Type Level Pattern Image Note
TS27: coronary artery endothelial cell of coronary artery Present 2N
TS27: coronary vascular plexus endothelial cell Present 2N Expressed in the capillaries.
Specimen 2O: postnatal newborn; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Wt1-cre)#Jbeb/0 (more )
Note: Conditional mutant. Triple labeled: red - Pecam1; green - Cre reporter; blue - Acta2 (assay MGI:7431145).
Structure Cell Type Level Pattern Image Note
TS27: coronary artery endothelial cell of coronary artery Present 2O
Specimen 3B: embryonic day 17.5; Wt1tm1.1Ndha/Wt1tm1.1Ndha (more )
Note: Double labeled: red - Pecam1; green - Acta2 (assay MGI:7431189).
Structure Cell Type Level Pattern Image Note
TS25: coronary artery endothelial cell of coronary artery Present 3B
TS25: endocardial lining endocardial cell Present 3B
Specimen 3E: embryonic day 17.5; Wt1tm1.1Ndha/Wt1tm1.1Ndha, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Acta2 (assay MGI:7431189).
Structure Cell Type Level Pattern Image Note
TS25: endocardial lining endocardial cell Present 3E Normal coronary artery was missing in the mutants, which also displayed a thin ventricular compact myocardium.
Specimen S1E: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS20: sinus venosus endocardial cell Present S1E
Specimen S1F: embryonic day 12.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS20: ventricle endocardial lining endocardial cell Present S1F
Specimen S2B/C: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: proepicardium Weak S2B , S2C Expressed in the differentiated developing coronary endothelium cells in the dorsal region.
Specimen S3B: embryonic day 9.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Wt1-cre)#Jbeb/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS15: heart blood vessel endothelial cell Present S3B
Specimen S3C: embryonic day 11.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Wt1-cre)#Jbeb/0 (more )
Note: Conditional mutant. Double labeled: red - Pecam1; green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS19: endocardial lining endocardial cell Present S3C
TS19: heart blood vessel endothelial cell Present S3C
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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