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Gene Expression Data
Assay Details
Assay
Reference: J:229936 Cano E, et al., Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections. Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):656-61
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7431145
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody
Results
Specimen 1H: embryonic day 14.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant. Triple labeled: blue - Acta2; green - Cre reporter; red - Pecam1 (assay MGI:7431138).
Structure Cell Type Level Pattern Image Note
TS22: coronary artery smooth muscle cell of the coronary artery Present 1H
TS22: myocardium layer cardiac muscle cell Present 1H

Specimen 1J: embryonic day 18.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Gata4*G2-cre)#Roja/0 (more )
Note: Conditional mutant. Triple labeled: blue - Acta2; green - Cre reporter; red - Pecam1 (assay MGI:7431138).
Structure Cell Type Level Pattern Image Note
TS26: coronary artery smooth muscle cell of the coronary artery Present 1J

Specimen 2F: embryonic day 13.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Triple labeled: blue - Acta2; red - Pecam1 (assay MGI:7431138); green - Wt1 reporter (assay MGI:7431230).
Structure Cell Type Level Pattern Image Note
TS21: coronary artery smooth muscle cell of the coronary artery Present 2F
TS21: myocardium layer cardiac muscle cell Present 2F

Specimen 2G: embryonic day 14.5; Wt1tm1Nhsn/Wt1+ (more )
Note: Triple labeled: blue - Acta2; red - Pecam1 (assay MGI:7431138); green - Wt1 reporter (assay MGI:7431230).
Structure Cell Type Level Pattern Image Note
TS22: myocardium layer cardiac muscle cell Present 2G

Specimen 2O: postnatal newborn; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Wt1-cre)#Jbeb/0 (more )
Note: Conditional mutant. Triple labeled: blue - Acta2; red - Pecam1 (assay MGI:7431138); green - Cre reporter.
Structure Cell Type Level Pattern Image Note
TS27: coronary artery smooth muscle cell of the coronary artery Present 2O


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/28/2024
MGI 6.13
The Jackson Laboratory