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Gene Expression Data
Assay Details
Assay
Reference: J:278916 Maruyama K, et al., Isl1-expressing non-venous cell lineage contributes to cardiac lymphatic vessel development. Dev Biol. 2019 Aug 15;452(2):134-143
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6758952
Gene symbol: Lyve1
Gene name: lymphatic vessel endothelial hyaluronan receptor 1
Antibody: anti-LYVE1 (AF2125)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 1A: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; blue - Pecam1 (assay MGI:6758967); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1A LYVE1 , 1A merge Expressed extensively in cardiac lymphatic vessels.
TS26: lymphatic vessel endothelium Present Regionally restricted 1A LYVE1 , 1A merge Expressed extensively in cardiac lymphatic vessels.
Specimen 1B: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; blue - Pecam1 (assay MGI:6758967); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1B LYVE1 , 1B merge Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
TS26: lymphatic vessel endothelium Present Regionally restricted 1B LYVE1 , 1B merge Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
Specimen 1C: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; blue - Pecam1 (assay MGI:6758967); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: heart Present 1C LYVE1 , 1C merge Expressed in cardiac lymphatic vessels.
TS26: lymphatic vessel endothelium Present 1C LYVE1 , 1C merge Expressed in cardiac lymphatic vessels.
Specimen 1D: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; blue - Pecam1 (assay MGI:6758967); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1D LYVE1 , 1D merge Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
TS26: lymphatic vessel endothelium Present Regionally restricted 1D LYVE1 , 1D merge Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
Specimen 1E: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; blue - Pecam1 (assay MGI:6758967); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1E merge , 1E LYVE1 Expressed extensively in cardiac lymphatic vessels.
TS26: lymphatic vessel endothelium Present Regionally restricted 1E merge , 1E LYVE1 Expressed extensively in cardiac lymphatic vessels.
Specimen 1F: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; blue - Pecam1 (assay MGI:6758967); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: heart Present Regionally restricted 1F merge , 1F LYVE1 Expressed in hypoplastic cardiac lymphatic vessels. Scarce lymphatic vessels on the dorsal ventricular wall.
TS26: lymphatic vessel endothelium Present Regionally restricted 1F merge , 1F LYVE1 Expressed in hypoplastic cardiac lymphatic vessels. Scarce lymphatic vessels on the dorsal ventricular wall.
Specimen 3A/B: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3B , 3A Expressed in cardiac lymphatic vessels.
Specimen 3C/D: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3D , 3C Expressed in cardiac lymphatic vessels.
Specimen 3E/F: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3E , 3F Decreased expression in impaired lymphatic vessels particularly on the ventral side.
Specimen 3G/H: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3H , 3G Decreased expression in impaired lymphatic vessels particularly on the ventral side.
Specimen 3I/J: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3J LYVE1 , 3I , 3J Merge Expressed in lymphatic vessels around the aorta on the ventral side of the heart.
Specimen 3K/L: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted 3K , 3L LYVE1 , 3L Merge Decreased expression in impaired lymphatic vessels and did not apparently form mature thick vessels.
Specimen 6A: embryonic day 12.0 (more )
Note: Left lateral. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6758996); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS20: lymphatic system Present Regionally restricted 6A LYVE1 , 6A Prox1-LYVE1 , 6A Merge Expressed in vessels around the sinus venosus. No coexpression with Prox1 mostly.
Specimen S1D fl/+: embryonic day 17.5; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Right lateral. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: heart Present Regionally restricted S1D left Expressed in cardiac lymphatic vessels.
TS25: lymphatic vessel endothelium Present Regionally restricted S1D left Expressed in cardiac lymphatic vessels.
Specimen S1D fl/fl: embryonic day 17.5; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Right lateral. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: heart Present Regionally restricted S1D right Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
TS25: lymphatic vessel endothelium Present Regionally restricted S1D right Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
Specimen S1E/F fl/+: embryonic day 17.5; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: heart Present Regionally restricted S1F LYVE1 left , S1E left , S1F merge left Expressed in cardiac lymphatic vessels.
TS25: lymphatic vessel endothelium Present Regionally restricted S1F LYVE1 left , S1E left , S1F merge left Expressed in cardiac lymphatic vessels.
Specimen S1E/F fl/fl: embryonic day 17.5; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; green - Prox1 (assay MGI:6759001); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS25: heart Present Regionally restricted S1E right , S1F merge right , S1F LYVE1 right Expressed in hypoplastic cardiac lymphatic vessels. Aberrant Lyve1-negative microvasculature was formed by Foxa1+/Pecam1+ cells around the aortic stem sites.
TS25: lymphatic vessel endothelium Present Regionally restricted S1E right , S1F merge right , S1F LYVE1 right Expressed in hypoplastic cardiac lymphatic vessels. Aberrant Lyve1-negative microvasculature was formed by Foxa1+/Pecam1+ cells around the aortic stem sites.
Specimen S3A/B/C: embryonic day 17.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Ventral side. Triple labeled: red - Lyve1; blue - Prox1 (assay MGI:6758992); green - Cre reporter.
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted S3C LYVE1 , S3A , S3C merge , S3B Colocalized with Isl1+ lineage and Prox1 around the outflow tract.
Specimen S3D/E: embryonic day 17.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Dorsal side. Triple labeled: red - Lyve1; blue - Prox1 (assay MGI:6758992); green - Cre reporter.
Structure Level Pattern Image Note
TS25: lymphatic vessel endothelium Present Regionally restricted S3D , S3E merge , S3E LYVE1 Colocalized with Prox1, not the Isl1+ lineage on the dorsal side of the heart.
Specimen S5A/B: embryonic day 14.5 (more )
Note: Ventral side. Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS22: arch of aorta Present Scattered S5B merge , S5A , S5B LYVE1 Sparsely coexpressed with Adgre1 (likely macrophages) on the aortic wall.
TS22: lymphatic vessel endothelium Present S5B merge , S5A , S5B LYVE1
Specimen S5C: embryonic day 14.5 (more )
Note: Dorsal side. Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS22: heart Present Scattered S5C merge , S5C LYVE1 A small number of Adgre1+/Lyve1+ macrophages were identified around the sinus venosus.
TS22: lymphatic vessel endothelium Present S5C merge , S5C LYVE1
Specimen S5D/E: embryonic day 14.5 (more )
Note: Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS22: visceral pericardium Present Scattered S5E LYVE1 , S5D , S5E merge Sparsely coexpressed with Lyve1 in macrophages.
TS22: lymphatic vessel endothelium Present S5E LYVE1 , S5E merge , S5D
Specimen S5F/G: embryonic day 15.5 (more )
Note: Ventral side. Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS23: aorta Present Scattered S5F , S5G merge , S5G LYVE1 Coexpressed with Adgre1 in macrophages.
TS23: lymphatic vessel endothelium Present S5G merge , S5G LYVE1 , S5F
Specimen S5H: embryonic day 15.5 (more )
Note: Dorsal side. Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS23: visceral pericardium Present Scattered S5H LYVE1 , S5H merge Coexpressed with Adgre1 in macrophages.
TS23: lymphatic vessel endothelium Present S5H LYVE1 , S5H merge
Specimen S5I/J: embryonic day 15.5 (more )
Note: Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS23: visceral pericardium Present Scattered S5I , S5J merge , S5J LYVE1 Coexpressed with Adgre1 in macrophages.
TS23: lymphatic vessel endothelium Present S5J merge , S5J LYVE1 , S5I
Specimen S5K/L: embryonic day 16.5 (more )
Note: Ventral side. Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS24: aorta Present Scattered S5L LYVE1 , S5L merge , S5K Coexpressed with Adgre1 in macrophages.
TS24: lymphatic vessel endothelium Present S5L merge , S5K , S5L LYVE1
Specimen S5M: embryonic day 16.5 (more )
Note: Dorsal side. Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS24: visceral pericardium Present Scattered S5M merge , S5M LYVE1 Coexpressed with Adgre1 in macrophages.
TS24: lymphatic vessel endothelium Present S5M LYVE1 , S5M merge
Specimen S5N/O: embryonic day 16.5 (more )
Note: Double labeled: red - Lyve1; green - Adgre1 (assay MGI:6758995).
Structure Level Pattern Image Note
TS24: visceral pericardium Present Scattered S5O merge , S5N , S5O LYVE1 Coexpressed with Adgre1 in macrophages.
TS24: lymphatic vessel endothelium Present S5N , S5O merge , S5O LYVE1
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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