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Gene Expression Data
Assay Details
Assay
Reference: J:278916 Maruyama K, et al., Isl1-expressing non-venous cell lineage contributes to cardiac lymphatic vessel development. Dev Biol. 2019 Aug 15;452(2):134-143
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6758967
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; red - Lyve1 (assay MGI:6758952); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: aorta Present Regionally restricted 1A PECAM , 1A merge Expressed in endothelial cells.
TS26: aortic sinus Present Regionally restricted 1A PECAM , 1A merge Expressed in endothelial cells.
TS26: heart blood vessel Present Regionally restricted 1A PECAM , 1A merge Expressed in endothelial cells.
TS26: pulmonary artery Present Regionally restricted 1A PECAM , 1A merge Expressed in endothelial cells.
TS26: lymphatic vessel endothelium Present 1A PECAM , 1A merge
Specimen 1B: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; red - Lyve1 (assay MGI:6758952); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: aorta Present Regionally restricted 1B PECAM , 1B merge Expressed in endothelial cells.
TS26: aortic sinus Present Regionally restricted 1B PECAM , 1B merge Expressed in endothelial cells.
TS26: heart blood vessel Present Regionally restricted 1B PECAM , 1B merge Expressed in endothelial cells.
TS26: pulmonary artery Present Regionally restricted 1B PECAM , 1B merge Expressed in endothelial cells.
TS26: lymphatic vessel endothelium Present 1B PECAM , 1B merge
Specimen 1C: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Lyve1 (assay MGI:6758952); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: aortic sinus Present Regionally restricted 1C PECAM , 1C merge Expressed in endothelial cells.
TS26: pulmonary artery Present Regionally restricted 1C PECAM , 1C merge Expressed in endothelial cells.
TS26: lymphatic vessel endothelium Present 1C PECAM , 1C merge
Specimen 1D: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Lyve1 (assay MGI:6758952); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: aorta Present Regionally restricted 1D PECAM , 1D merge Expressed in endothelial cells.
TS26: pulmonary artery Present Regionally restricted 1D PECAM , 1D merge Expressed in endothelial cells.
TS26: lymphatic vessel endothelium Present 1D merge , 1D PECAM
Specimen 1E: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: blue - Pecam1; red - Lyve1 (assay MGI:6758952); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: aorta Present Regionally restricted 1E merge , 1E PECAM Expressed in endothelial cells.
TS26: heart blood vessel Present Regionally restricted 1E merge , 1E PECAM Expressed in endothelial cells.
TS26: pulmonary artery Present Regionally restricted 1E merge , 1E PECAM Expressed in endothelial cells.
Specimen 1F: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: blue - Pecam1; red - Lyve1 (assay MGI:6758952); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: aorta Present Regionally restricted 1F PECAM , 1F merge Expressed in endothelial cells.
TS26: heart blood vessel Present Regionally restricted 1F PECAM , 1F merge Expressed in endothelial cells.
TS26: pulmonary artery Present Regionally restricted 1F PECAM , 1F merge Expressed in endothelial cells.
Specimen 2A/B: embryonic day 11.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Prox1 (assay MGI:6758976); green - Cre reporter.
Structure Level Pattern Image Note
TS19: 2nd branchial arch Present 2A , 2B
TS19: ascending aorta Present 2A , 2B
Specimen 2C: embryonic day 11.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Prox1 (assay MGI:6758976); green - Cre reporter.
Structure Level Pattern Image Note
TS19: common cardinal vein Present 2C Merge , 2C PECAM
TS19: heart atrium Present 2C Merge , 2C PECAM
Specimen 2D/E: embryonic day 13.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Prox1 (assay MGI:6758976); green - Cre reporter.
Structure Level Pattern Image Note
TS21: ascending aorta Present 2D , 2E
TS21: pulmonary trunk Present 2D
Specimen 2F/G: embryonic day 17.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Prox1 (assay MGI:6758976); green - Cre reporter.
Structure Level Pattern Image Note
TS25: aorta Present 2F
TS25: pulmonary artery Present 2F , 2G
Specimen 3A/B: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: heart blood vessel Present 3B , 3A
Specimen 3C/D: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Dorsal side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: heart blood vessel Present 3D , 3C
Specimen 3E/F: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: heart blood vessel Present 3F , 3E
Specimen 3G/H: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Dorsal side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: heart blood vessel Present 3G , 3H
Specimen 3I/J: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present 3I , 3J Merge
TS25: pulmonary artery Present 3I , 3J Merge
Specimen 3K/L: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present 3K , 3L Merge
TS25: pulmonary artery Present 3K , 3L Merge
Specimen 4G: embryonic day 9.5 (more )
Note: Triple labeled: blue - Pecam1; red - Isl1 (assay MGI:6758993); green - Prox1 (assay MGI:6758996).
Structure Level Pattern Image Note
TS15: blood vessel Present 4G Merge , 4G Pecam
Specimen 5A/B: embryonic day 9.5 (more )
Note: Left lateral. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Flt4 (assay MGI:6758981).
Structure Level Pattern Image Note
TS15: blood vessel Present 5B Merge , 5A
TS15: 1st branchial arch Present 5A Flt4+/Pecam1+ cells were located mainly in the periphery of the Prox1+ cell cluster, with partial co-expression of Prox1.
TS15: 2nd branchial arch Present 5A , 5B Merge Flt4+/Pecam1+ cells were located mainly in the periphery of the Prox1+ cell cluster, with partial co-expression of Prox1.
Specimen 5C/D: embryonic day 10.5 (more )
Note: Left lateral. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Flt4 (assay MGI:6758981).
Structure Level Pattern Image Note
TS17: blood vessel endothelium Present 5D Merge , 5C
TS17: 1st branchial arch Present 5D Merge , 5C Flt4+/Pecam1+ cells with and without Prox1 expression appeared to form mesh-like network structures, which extended caudally.
TS17: 2nd branchial arch Present 5D Merge , 5C Flt4+/Pecam1+ cells with and without Prox1 expression appeared to form mesh-like network structures, which extended caudally.
Specimen 5E/F: embryonic day 12.5 (more )
Note: Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Flt4 (assay MGI:6758981).
Structure Level Pattern Image Note
TS20: ascending aorta Present 5E , 5F Merge
TS20: pulmonary trunk Present 5E , 5F Merge
Specimen 5G/H: embryonic day 15.0 (more )
Note: Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Flt4 (assay MGI:6758981).
Structure Level Pattern Image Note
TS23: aorta Present 5H Merge , 5G
TS23: pulmonary trunk Present 5H Merge , 5G
Specimen 5I/J: embryonic day 16.5 (more )
Note: Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Flt4 (assay MGI:6758981).
Structure Level Pattern Image Note
TS24: aorta Present 5J Merge , 5I
TS24: pulmonary trunk Present 5J Merge , 5I
Specimen 6A: embryonic day 12.0 (more )
Note: Left lateral. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS19: 2nd branchial arch Present 6A Merge
TS19: sinus venosus Present 6A Merge
Specimen 6I: embryonic day 15.0 (more )
Note: Dorsal side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS23: lymphatic vessel endothelium Present Regionally restricted 6I left On the dorsal side of the hearts, Pecam1+/Prox1+/Flt4+ mature cardiac lymphatic vessels extended over the ventricular wall.
Specimen S1D fl/+: embryonic day 17.5; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Right lateral. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present Regionally restricted S1D left Expressed in endothelial cells.
TS25: heart blood vessel Present Regionally restricted S1D left Expressed in endothelial cells.
TS25: pulmonary artery Present Regionally restricted S1D left Expressed in endothelial cells.
TS25: lymphatic vessel endothelium Present Regionally restricted S1D left Expressed in cardiac lymphatic vessels.
Specimen S1D fl/fl: embryonic day 17.5; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Right lateral. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present Regionally restricted S1D right Expressed in endothelial cells.
TS25: heart blood vessel Present Regionally restricted S1D right Expressed in endothelial cells.
TS25: pulmonary artery Present Regionally restricted S1D right Expressed in endothelial cells.
TS25: lymphatic vessel endothelium Present S1D right Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
Specimen S1E/F fl/+: embryonic day 17.5; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present Regionally restricted S1E left Expressed in endothelial cells.
TS25: heart blood vessel Present Regionally restricted S1E left Expressed in endothelial cells.
TS25: pulmonary artery Present Regionally restricted S1E left Expressed in endothelial cells.
TS25: lymphatic vessel endothelium Present Regionally restricted S1F PECAM left , S1F merge left , S1E left Expressed in cardiac lymphatic vessels.
Specimen S1E/F fl/fl: embryonic day 17.5; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present Regionally restricted S1E right Expressed in endothelial cells.
TS25: heart blood vessel Present Regionally restricted S1E right Expressed in endothelial cells.
TS25: pulmonary artery Present Regionally restricted S1E right Expressed in endothelial cells.
TS25: lymphatic vessel endothelium Present Regionally restricted S1E right , S1F merge right , S1F PECAM right Aberrant Lyve1-negative microvasculature was formed by Foxa1+/Pecam1+ cells around the aortic stem sites.
Specimen S2B/C: embryonic day 13.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Prox1 (assay MGI:6758976); green - Cre reporter.
Structure Level Pattern Image Note
TS21: common carotid artery Present Regionally restricted S2C merge , S2C PECAM , S2B Expressed in endothelial cells.
TS21: jugular vein Present Regionally restricted S2C merge , S2C PECAM , S2B Expressed in endothelial cells.
TS21: lymph sac Present Regionally restricted S2C merge , S2C PECAM , S2B Expressed in endothelial cells in the jugular lymph sac.
Specimen S4G/H: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Prox1 (assay MGI:6758976); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS25: face skin Present Regionally restricted S4H PECAM , S4H merge , S4G Expressed in facial skin lymphatic vessels.
TS25: lymphatic vessel endothelium Present Regionally restricted S4H PECAM , S4H merge , S4G Expressed in facial skin lymphatic vessels.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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