About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:278916 Maruyama K, et al., Isl1-expressing non-venous cell lineage contributes to cardiac lymphatic vessel development. Dev Biol. 2019 Aug 15;452(2):134-143
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6758967
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; red - Lyve1 (assay MGI:6758952); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: aorta Present Regionally restricted 1A PECAM , 1A merge Expressed in endothelial cells.
TS26: aortic sinus Present Regionally restricted 1A PECAM , 1A merge Expressed in endothelial cells.
TS26: heart blood vessel Present Regionally restricted 1A PECAM , 1A merge Expressed in endothelial cells.
TS26: pulmonary artery Present Regionally restricted 1A PECAM , 1A merge Expressed in endothelial cells.
TS26: lymphatic vessel endothelium Present 1A PECAM , 1A merge
Specimen 1B: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; red - Lyve1 (assay MGI:6758952); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: aorta Present Regionally restricted 1B PECAM , 1B merge Expressed in endothelial cells.
TS26: aortic sinus Present Regionally restricted 1B PECAM , 1B merge Expressed in endothelial cells.
TS26: heart blood vessel Present Regionally restricted 1B PECAM , 1B merge Expressed in endothelial cells.
TS26: pulmonary artery Present Regionally restricted 1B PECAM , 1B merge Expressed in endothelial cells.
TS26: lymphatic vessel endothelium Present 1B PECAM , 1B merge
Specimen 1C: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Lyve1 (assay MGI:6758952); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: aortic sinus Present Regionally restricted 1C PECAM , 1C merge Expressed in endothelial cells.
TS26: pulmonary artery Present Regionally restricted 1C PECAM , 1C merge Expressed in endothelial cells.
TS26: lymphatic vessel endothelium Present 1C PECAM , 1C merge
Specimen 1D: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Lyve1 (assay MGI:6758952); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: aorta Present Regionally restricted 1D PECAM , 1D merge Expressed in endothelial cells.
TS26: pulmonary artery Present Regionally restricted 1D PECAM , 1D merge Expressed in endothelial cells.
TS26: lymphatic vessel endothelium Present 1D merge , 1D PECAM
Specimen 1E: embryonic day 18.0; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: blue - Pecam1; red - Lyve1 (assay MGI:6758952); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: aorta Present Regionally restricted 1E merge , 1E PECAM Expressed in endothelial cells.
TS26: heart blood vessel Present Regionally restricted 1E merge , 1E PECAM Expressed in endothelial cells.
TS26: pulmonary artery Present Regionally restricted 1E merge , 1E PECAM Expressed in endothelial cells.
Specimen 1F: embryonic day 18.0; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Dorsal side. Triple labeled: blue - Pecam1; red - Lyve1 (assay MGI:6758952); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS26: aorta Present Regionally restricted 1F PECAM , 1F merge Expressed in endothelial cells.
TS26: heart blood vessel Present Regionally restricted 1F PECAM , 1F merge Expressed in endothelial cells.
TS26: pulmonary artery Present Regionally restricted 1F PECAM , 1F merge Expressed in endothelial cells.
Specimen 2A/B: embryonic day 11.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Prox1 (assay MGI:6758976); green - Cre reporter.
Structure Level Pattern Image Note
TS19: 2nd branchial arch Present 2A , 2B
TS19: ascending aorta Present 2A , 2B
Specimen 2C: embryonic day 11.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Prox1 (assay MGI:6758976); green - Cre reporter.
Structure Level Pattern Image Note
TS19: common cardinal vein Present 2C Merge , 2C PECAM
TS19: heart atrium Present 2C Merge , 2C PECAM
Specimen 2D/E: embryonic day 13.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Prox1 (assay MGI:6758976); green - Cre reporter.
Structure Level Pattern Image Note
TS21: ascending aorta Present 2D , 2E
TS21: pulmonary trunk Present 2D
Specimen 2F/G: embryonic day 17.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Prox1 (assay MGI:6758976); green - Cre reporter.
Structure Level Pattern Image Note
TS25: aorta Present 2F
TS25: pulmonary artery Present 2F , 2G
Specimen 3A/B: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: heart blood vessel Present 3B , 3A
Specimen 3C/D: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Dorsal side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: heart blood vessel Present 3D , 3C
Specimen 3E/F: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: heart blood vessel Present 3F , 3E
Specimen 3G/H: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Dorsal side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: heart blood vessel Present 3G , 3H
Specimen 3I/J: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present 3I , 3J Merge
TS25: pulmonary artery Present 3I , 3J Merge
Specimen 3K/L: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1tm1.1Fuma (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present 3K , 3L Merge
TS25: pulmonary artery Present 3K , 3L Merge
Specimen 4G: embryonic day 9.5 (more )
Note: Triple labeled: blue - Pecam1; red - Isl1 (assay MGI:6758993); green - Prox1 (assay MGI:6758996).
Structure Level Pattern Image Note
TS15: blood vessel Present 4G Merge , 4G Pecam
Specimen 5A/B: embryonic day 9.5 (more )
Note: Left lateral. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Flt4 (assay MGI:6758981).
Structure Level Pattern Image Note
TS15: blood vessel Present 5B Merge , 5A
TS15: 1st branchial arch Present 5A Flt4+/Pecam1+ cells were located mainly in the periphery of the Prox1+ cell cluster, with partial co-expression of Prox1.
TS15: 2nd branchial arch Present 5A , 5B Merge Flt4+/Pecam1+ cells were located mainly in the periphery of the Prox1+ cell cluster, with partial co-expression of Prox1.
Specimen 5C/D: embryonic day 10.5 (more )
Note: Left lateral. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Flt4 (assay MGI:6758981).
Structure Level Pattern Image Note
TS17: blood vessel endothelium Present 5D Merge , 5C
TS17: 1st branchial arch Present 5D Merge , 5C Flt4+/Pecam1+ cells with and without Prox1 expression appeared to form mesh-like network structures, which extended caudally.
TS17: 2nd branchial arch Present 5D Merge , 5C Flt4+/Pecam1+ cells with and without Prox1 expression appeared to form mesh-like network structures, which extended caudally.
Specimen 5E/F: embryonic day 12.5 (more )
Note: Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Flt4 (assay MGI:6758981).
Structure Level Pattern Image Note
TS20: ascending aorta Present 5E , 5F Merge
TS20: pulmonary trunk Present 5E , 5F Merge
Specimen 5G/H: embryonic day 15.0 (more )
Note: Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Flt4 (assay MGI:6758981).
Structure Level Pattern Image Note
TS23: aorta Present 5H Merge , 5G
TS23: pulmonary trunk Present 5H Merge , 5G
Specimen 5I/J: embryonic day 16.5 (more )
Note: Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Flt4 (assay MGI:6758981).
Structure Level Pattern Image Note
TS24: aorta Present 5J Merge , 5I
TS24: pulmonary trunk Present 5J Merge , 5I
Specimen 6A: embryonic day 12.0 (more )
Note: Left lateral. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS19: 2nd branchial arch Present 6A Merge
TS19: sinus venosus Present 6A Merge
Specimen 6I: embryonic day 15.0 (more )
Note: Dorsal side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6758996); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS23: lymphatic vessel endothelium Present Regionally restricted 6I left On the dorsal side of the hearts, Pecam1+/Prox1+/Flt4+ mature cardiac lymphatic vessels extended over the ventricular wall.
Specimen S1D fl/+: embryonic day 17.5; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Right lateral. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present Regionally restricted S1D left Expressed in endothelial cells.
TS25: heart blood vessel Present Regionally restricted S1D left Expressed in endothelial cells.
TS25: pulmonary artery Present Regionally restricted S1D left Expressed in endothelial cells.
TS25: lymphatic vessel endothelium Present Regionally restricted S1D left Expressed in cardiac lymphatic vessels.
Specimen S1D fl/fl: embryonic day 17.5; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Right lateral. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present Regionally restricted S1D right Expressed in endothelial cells.
TS25: heart blood vessel Present Regionally restricted S1D right Expressed in endothelial cells.
TS25: pulmonary artery Present Regionally restricted S1D right Expressed in endothelial cells.
TS25: lymphatic vessel endothelium Present S1D right Expressed in hypoplastic cardiac lymphatic vessels. Relatively preserved.
Specimen S1E/F fl/+: embryonic day 17.5; Prox1tm1.1Fuma/Prox1+, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present Regionally restricted S1E left Expressed in endothelial cells.
TS25: heart blood vessel Present Regionally restricted S1E left Expressed in endothelial cells.
TS25: pulmonary artery Present Regionally restricted S1E left Expressed in endothelial cells.
TS25: lymphatic vessel endothelium Present Regionally restricted S1F PECAM left , S1F merge left , S1E left Expressed in cardiac lymphatic vessels.
Specimen S1E/F fl/fl: embryonic day 17.5; Prox1tm1.1Fuma/Prox1tm1.1Fuma, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Ventral side. Triple labeled: blue - Pecam1; green - Prox1 (assay MGI:6759001); red - Lyve1 (assay MGI:6758952).
Structure Level Pattern Image Note
TS25: aorta Present Regionally restricted S1E right Expressed in endothelial cells.
TS25: heart blood vessel Present Regionally restricted S1E right Expressed in endothelial cells.
TS25: pulmonary artery Present Regionally restricted S1E right Expressed in endothelial cells.
TS25: lymphatic vessel endothelium Present Regionally restricted S1E right , S1F merge right , S1F PECAM right Aberrant Lyve1-negative microvasculature was formed by Foxa1+/Pecam1+ cells around the aortic stem sites.
Specimen S2B/C: embryonic day 13.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Prox1 (assay MGI:6758976); green - Cre reporter.
Structure Level Pattern Image Note
TS21: common carotid artery Present Regionally restricted S2C merge , S2C PECAM , S2B Expressed in endothelial cells.
TS21: jugular vein Present Regionally restricted S2C merge , S2C PECAM , S2B Expressed in endothelial cells.
TS21: lymph sac Present Regionally restricted S2C merge , S2C PECAM , S2B Expressed in endothelial cells in the jugular lymph sac.
Specimen S4G/H: embryonic day 17.5; Isl1tm1(cre)Sev/Isl1+, Prox1tm1.1Fuma/Prox1+ (more )
Note: Conditional mutant. Triple labeled: blue - Pecam1; red - Prox1 (assay MGI:6758976); green - Prox1 (assay MGI:6759001).
Structure Level Pattern Image Note
TS25: face skin Present Regionally restricted S4H PECAM , S4H merge , S4G Expressed in facial skin lymphatic vessels.
TS25: lymphatic vessel endothelium Present Regionally restricted S4H PECAM , S4H merge , S4G Expressed in facial skin lymphatic vessels.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/21/2024
MGI 6.23 		The Jackson Laboratory