Pdx1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:158780 Li S, et al., SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells. BMC Dev Biol. 2010;10:19
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6479966
Gene symbol:	Pdx1
Gene name:	pancreatic and duodenal homeobox 1

Antibody:	anti-Pdx1
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. Anti-Pdx1 MGI:6479915 may have been used in this assay.

Results

1E ©

Specimen 1E: embryonic day 14.5; Sel1l^{Gt(CA0017)Wtsi}/Sel1l⁺ (more

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Note: Double labeled: green - Pdx1; red - Sel1l reporter (assay MGI:6479960).

Structure	Level	Pattern	Image	Note
TS22: pancreas epithelium	Present		1E

1L ©

Specimen 1L: postnatal day 1; Sel1l^{Gt(CA0017)Wtsi}/Sel1l⁺ (more

)
Note: Double labeled: green - Pdx1; red - Sel1l reporter (assay MGI:6479960).

Structure	Level	Pattern	Image	Note
TS27: pancreas	Present		1L

2A ©

Specimen 2A: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium dorsal bud	Present		2A
TS19: pancreas primordium ventral bud	Present		2A

2B ©

Specimen 2B: embryonic day 11.5; Sel1l^{Gt(CA0017)Wtsi}/Sel1l^{Gt(CA0017)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Present	Regionally restricted	2B	Dorsal and pancreatic buds appear to be fused together.

2C ©

Specimen 2C: embryonic day 13.5 (more

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Note: Double labeled: green - Pdx1; red - Ins1 (assay MGI:6479964) and Ins2 (assay MGI:6479965).

Structure	Level	Pattern	Image	Note
TS21: pancreas epithelium	Present		2C

2D ©

Specimen 2D: embryonic day 13.5; Sel1l^{Gt(CA0017)Wtsi}/Sel1l^{Gt(CA0017)Wtsi} (more

)
Note: Double labeled: green - Pdx1; red - Ins1 (assay MGI:6479964) and Ins2 (assay MGI:6479965).

Structure	Level	Pattern	Image	Note
TS21: pancreas epithelium	Present	Regionally restricted	2D	Dorsal pancreatic bud exhibits markedly reduced epithelial size and impaired branching morphoogy.

3A ©

Specimen 3A: embryonic day 11.5 (more

)
Note: Double labeled: green - Pdx1; red - glucagon (Gcg; assay MGI:6479963).

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium epithelium	Present		3A

3B ©

Specimen 3B: embryonic day 11.5; Sel1l^{Gt(CA0017)Wtsi}/Sel1l^{Gt(CA0017)Wtsi} (more

)
Note: Double labeled: green - Pdx1; red - glucagon (Gcg; assay MGI:6479963).

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium epithelium	Present		3B

3C ©

Specimen 3C: embryonic day 15.5 (more

)
Note: Double labeled: green - Pdx1; red - Ins1 (assay MGI:6479964) and Ins2 (assay MGI:6479965).

Structure	Level	Pattern	Image	Note
TS23: pancreas epithelium	Present		3C

3D ©

Specimen 3D: embryonic day 15.5; Sel1l^{Gt(CA0017)Wtsi}/Sel1l^{Gt(CA0017)Wtsi} (more

)
Note: Double labeled: green - Pdx1; red - Ins1 (assay MGI:6479964) and Ins2 (assay MGI:6479965).

Structure	Level	Pattern	Image	Note
TS23: pancreas epithelium	Present		3D

5A ©

Specimen 5A: embryonic day 15.5 (more

)
Note: Double labeled: green - Pdx1; red - Ptf1a (assay MGI:6479969).

Structure	Level	Pattern	Image	Note
TS23: pancreas epithelium	Present	Graded	5A	Differential expression in pancreatic epithelial cells, with high expression in subset of core epithelial cells and low expression in peripheral cells.

5B ©

Specimen 5B: embryonic day 15.5; Sel1l^{Gt(CA0017)Wtsi}/Sel1l^{Gt(CA0017)Wtsi} (more

)
Note: Double labeled: green - Pdx1; red - Ptf1a (assay MGI:6479969).

Structure	Level	Pattern	Image	Note
TS23: pancreas epithelium	Present	Uniform	5B	Uniform expression in pancreatic epithelial cells.

5C ©

Specimen 5C: embryonic day 15.5 (more

)
Note: Double labeled: green - Pdx1; red - Sox9 (assay MGI:6479962).

Structure	Level	Pattern	Image	Note
TS23: pancreas epithelium	Present		5C

5D ©

Specimen 5D: embryonic day 15.5; Sel1l^{Gt(CA0017)Wtsi}/Sel1l^{Gt(CA0017)Wtsi} (more

)
Note: Double labeled: green - Pdx1; red - Sox9 (assay MGI:6479962).

Structure	Level	Pattern	Image	Note
TS23: pancreas epithelium	Present		5D

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