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Gene Expression Data
Assay Details
Assay
Reference: J:132598 Nishimura W, et al., Preferential reduction of beta cells derived from Pax6-MafB pathway in MafB deficient mice. Dev Biol. 2008 Feb 15;314(2):443-56
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6382861
Gene symbol: Nkx6-1
Gene name: NK6 homeobox 1
Antibody: anti-Nkx6.1
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 488
Results
Specimen S1D: embryonic day 15.5 (more )
Note: Double labeled: green - Nkx6-1; red - Ins1 (assay MGI:6382865) and Ins2 (assay MGI:6382879).
Structure Level Pattern Image Note
TS23: endocrine pancreas Present S1D Expression marks area of endocrine differentiation.
Specimen S1I: embryonic day 15.5; Pax6Sey-Neu/Pax6+ (more )
Note: Double labeled: green - Nkx6-1; red - Ins1 (assay MGI:6382865) and Ins2 (assay MGI:6382879).
Structure Level Pattern Image Note
TS23: endocrine pancreas Present S1I Expression unaffected compared with wild type.
Specimen 1A: embryonic day 15.5 (more )
Note: Double labeled: green - Nkx6-1; red - Ins1 (assay MGI:6382865) and Ins2 (assay MGI:6382879).
Structure Level Pattern Image Note
TS23: pancreas Present 1A
Specimen 1E: embryonic day 15.5; Pax6Sey-Neu/Pax6+ (more )
Note: Double labeled: green - Nkx6-1; red - Ins1 (assay MGI:6382865) and Ins2 (assay MGI:6382879).
Structure Level Pattern Image Note
TS23: pancreas Present 1E
Specimen 1I: embryonic day 17.5 (more )
Note: Double labeled: green - Nkx6-1; red - Ins1 (assay MGI:6382865) and Ins2 (assay MGI:6382879).
Structure Level Pattern Image Note
TS25: pancreas Present 1I
Specimen 1M: embryonic day 17.5; Pax6Sey-Neu/Pax6+ (more )
Note: Double labeled: green - Nkx6-1; red - Ins1 (assay MGI:6382865) and Ins2 (assay MGI:6382879).
Structure Level Pattern Image Note
TS25: pancreas Weak Regionally restricted 1M Majority of expression restricted to insulin-expressing cells.
Specimen 3A: embryonic day 12.5 (more )
Note: Double labeled: green - Nkx6-1; red - Gluc (Gcg assay MGI:6382866).
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted 3A No expression in majority of Gcg-expressing cells.
Specimen 3E: embryonic day 12.5; Pax6Sey-Neu/Pax6+ (more )
Note: Double labeled: green - Nkx6-1; red - Gluc (Gcg assay MGI:6382866).
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted 3E Authors report normal expression. No expression in majority of Gcg-expressing cells.
Specimen 7B: embryonic day 15.5 (more )
Note: Double labeled: green - Nkx6-1; red - Ins1 (assay MGI:6382863) and Ins2 (assay MGI:6382879).
Structure Level Pattern Image Note
TS23: pancreas Present 7B
Specimen 7F: embryonic day 15.5; Mafbm1Gsb/Mafbm1Gsb (more )
Note: Double labeled: green - Nkx6-1; red - Ins1 (assay MGI:6382863) and Ins2 (assay MGI:6382879).
Structure Level Pattern Image Note
TS23: pancreas Present 7F Expression not affected compared with wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory