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Gene Expression Data
Assay Details
Assay
Reference: J:273053 Shih HP, et al., A Gene Regulatory Network Cooperatively Controlled by Pdx1 and Sox9 Governs Lineage Allocation of Foregut Progenitor Cells. Cell Rep. 2015 Oct 13;13(2):326-36
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6361261
Gene symbol: Cdx2
Gene name: caudal type homeobox 2
Antibody: anti-Cdx2 (CDX2-88)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Two antibodies were described, but the one used in this assay was not specified; either MGI:3051329 or MGI:6361203 could have been used.
Results
Specimen 2B: embryonic day 8.75 (more )
Note: Triple labeled: green - Cdx2; red - Sox9 (assay MGI:6361259); white - Pdx1 (assay MGI:6361260).
Structure Level Pattern Image Note
TS13: foregut-midgut junction Present Non-Uniform 2B'' , 2B At the boundary between the duodenal and pre-pancreatic domains, there was a transition from a Cdx-high to a Cdx-low state.
Specimen 6A: embryonic day 10.5 (more )
Note: Double labeled: green - Cdx2; red - Pdx1 (assay MGI:6361315).
Structure Level Pattern Image Note
TS17: duodenum Present 6A' , 6A , 6A''
TS17: foregut-midgut junction Present Regionally restricted 6A'' , 6A , 6A' At the duodenal-pancreatic junction, the Pdx1+ domain forms a boundary with the Cdx2+ domain; only a few Pdx1+ cells co-express Cdx2.
Specimen 6B: embryonic day 10.5; Sox9tm1Gsr/Sox9+, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant. Double labeled: green - Cdx2; red - Pdx1 (assay MGI:6361315).
Structure Level Pattern Image Note
TS17: duodenum Present 6B' , 6B'' , 6B
TS17: foregut-midgut junction Present Regionally restricted 6B , 6B'' , 6B' At the duodenal-pancreatic junction, the Pdx1+ domain forms a boundary with the Cdx2+ domain; only a few Pdx1+ cells co-express Cdx2.
Specimen 6C: embryonic day 10.5; Pdx1tm2Cvw/Pdx1+ (more )
Note: Double labeled: green - Cdx2; red - Pdx1 (assay MGI:6361315).
Structure Level Pattern Image Note
TS17: duodenum Present 6C' , 6C , 6C''
TS17: foregut-midgut junction Present Regionally restricted 6C' , 6C , 6C'' At the duodenal-pancreatic junction, the Pdx1+ domain forms a boundary with the Cdx2+ domain; only a few Pdx1+ cells co-express Cdx2.
Specimen 6D: embryonic day 10.5; Pdx1tm2Cvw/Pdx1+, Sox9tm1Gsr/Sox9+, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant. Double labeled: green - Cdx2; red - Pdx1 (assay MGI:6361315).
Structure Level Pattern Image Note
TS17: duodenum Present 6D'' , 6D' , 6D
TS17: foregut-midgut junction Present Regionally restricted 6D'' , 6D , 6D' At the duodenal-pancreatic junction, the Pdx1+ domain forms a boundary with the Cdx2+ domain; only a few Pdx1+ cells co-express Cdx2.
Specimen 6E: embryonic day 10.5; Sox9tm1Gsr/Sox9tm1Gsr, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant. Double labeled: green - Cdx2; red - Pdx1 (assay MGI:6361315).
Structure Level Pattern Image Note
TS17: duodenum Present 6E , 6E'' , 6E'
TS17: foregut-midgut junction Present Regionally restricted 6E , 6E'' , 6E' At the duodenal-pancreatic junction, the Pdx1+ domain forms a boundary with the Cdx2+ domain; only a few Pdx1+ cells co-express Cdx2.
Specimen 6F: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw (more )
Note: Double labeled: green - Cdx2; red - Pdx1 (assay MGI:6361315).
Structure Level Pattern Image Note
TS17: duodenum Present 6F' , 6F , 6F''
TS17: foregut-midgut junction Present Regionally restricted 6F' , 6F'' , 6F At the duodenal-pancreatic junction, the Pdx1+ domain forms a boundary with the Cdx2+ domain; only a few Pdx1+ cells co-express Cdx2.
Specimen 6G: embryonic day 10.5; Pdx1tm2Cvw/Pdx1tm2Cvw, Sox9tm1Gsr/Sox9tm1Gsr, Tg(Foxa3-cre)1Khk/0 (more )
Note: Conditional mutant. Double labeled: green - Cdx2; red - Pdx1 (assay MGI:6361315).
Structure Level Pattern Image Note
TS17: duodenum Present 6G'' , 6G' , 6G
TS17: foregut-midgut junction Present Regionally restricted 6G' , 6G , 6G'' The loss of both Pdx1 and Sox9 results in ectopic Cdx2 expression. There was extensive overlap between the Pdx1 and Cdx2 domains.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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