Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:273053
Shih HP, et al., A Gene Regulatory Network Cooperatively Controlled by Pdx1 and Sox9 Governs Lineage Allocation of Foregut Progenitor Cells. Cell Rep. 2015 Oct 13;13(2):326-36
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6361259
|
|
Gene symbol:
|
Sox9
|
|
Gene name:
|
SRY (sex determining region Y)-box 9
|
|
Antibody:
|
anti-Sox9
|
|
Detection system:
|
Secondary antibody coupled to Cy5
|
|
Assay notes:
|
Two antibodies were described, but the one used in this assay was not specified; either MGI:3056750 or MGI:5317435 could have been used.
|
|
|
Results
|
Specimen
2A:
embryonic day 8.75
(more  )
Note: Triple labeled: red - Sox9; green - Sox2 (assay MGI:6361258); white - Pdx1 (assay MGI:6361260).
Specimen
2A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.75
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - Sox9; green - Sox2 (assay MGI:6361258); white - Pdx1 (assay MGI:6361260).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: foregut-midgut junction |
Present |
Regionally restricted |
2A
,
2A'
|
The Sox2-positive domain, from which the stomach develops, formed a boundary with both the Pdx1+ and Sox9+ domains. Very few cells co-expressing Sox2, Pdx1, and Sox9 were observed at this boundary. Sox9 staining spanned the proximal duodenal and pre-pancreatic domains.
|
Specimen
2B:
embryonic day 8.75
(more )
Note: Triple labeled: red - Sox9; green - Cdx2 (assay MGI:6361261); white - Pdx1 (assay MGI:6361260).
Specimen
2B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.75
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: red - Sox9; green - Cdx2 (assay MGI:6361261); white - Pdx1 (assay MGI:6361260).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: foregut-midgut junction |
Present |
Regionally restricted |
2B
,
2B'
|
Cells in the presumptive proximal duodenum expressed high levels of Sox9. Sox9 staining spanned the proximal duodenal and pre-pancreatic domains.
|
|
Analysis Tools