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Gene Expression Data
Assay Details
Assay
Reference: J:233088 Frame JM, et al., Definitive Hematopoiesis in the Yolk Sac Emerges from Wnt-Responsive Hemogenic Endothelium Independently of Circulation and Arterial Identity. Stem Cells. 2016 Feb;34(2):431-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6201387
Gene symbol: Kit
Gene name: KIT proto-oncogene receptor tyrosine kinase
Antibody: anti-Kit
Detection system: Secondary antibody
Assay notes: Fixative was either acetone or 2% formaldehyde.
Results
Specimen 1F: embryonic day 9.5 (more )
Note: 19 somite pairs. Double labeled: green - Kit; red - Itga2b (assay MGI:6201385).
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters 1F Kit , 1F Merge
Specimen 2A: embryonic day 8.5 (more )
Note: 7 somite pairs. Triple labeled: green - Kit; red - Pecam1 (assay MGI:6201388); blue - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS13: yolk sac Present Regionally restricted 2A Kit , 2A Merge Expression is in flattened hemogenic endothelial cells and in rounded hematopoietic cells.
Specimen 2B top left: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: green - Kit; red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 2B top left Expression is in flattened hemogenic endothelial cells and in rounded hematopoietic cells.
Specimen 2B top right: embryonic day 10.0 (more )
Note: 31 somite pairs. Double labeled: green - Kit; red - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS16: yolk sac Present Regionally restricted 2B top right Expression is in flattened hemogenic endothelial cells and in rounded hematopoietic cells.
Specimen 2B bottom left: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: green - Kit; red - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 2B bottom left Expression is in flattened hemogenic endothelial cells and in rounded hematopoietic cells.
Specimen 2B bottom right: embryonic day 9.5 (more )
Note: 28 somite pairs. Double labeled: green - Kit; red - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 2B bottom right Expression is in flattened hemogenic endothelial cells and in rounded hematopoietic cells.
Specimen 2C top left: embryonic day 8.5 (more )
Note: 8 somite pairs. Double labeled: green - Kit; red - Cdh5 (assay MGI:6201390).
Structure Level Pattern Image Note
TS13: yolk sac Present Clusters 2C top left
Specimen 2C top middle: embryonic day 9.5 (more )
Note: 19 somite pairs.
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters 2C top middle
Specimen 2C bottom left: embryonic day 8.0 (more )
Note: 6 somite pairs. Triple labeled: green - Kit; blue - Runx1 (assay MGI:6201389); red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS12: yolk sac Present Clusters 2C bottom left
Specimen 2C bottom middle: embryonic day 11.0 (more )
Note: 41 somite pairs. Double labeled: green - Kit; red - Cdh5 (assay MGI:6201390).
Structure Level Pattern Image Note
TS18: yolk sac Present Clusters 2C bottom middle
Specimen 2C bottom right: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: green - Kit; red - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters 2C right
Specimen 2D top: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: green - Kit; red - Kdr (assay MGI:6201396).
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters 2D top
Specimen 2D second: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: green - Kit; red - Kdr (assay MGI:6201396).
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters 2D second
Specimen 2D third: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: green - Kit; red - Kdr (assay MGI:6201396).
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters 2D third
Specimen 2D fourth: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: green - Kit; red - Kdr (assay MGI:6201396).
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters 2D fourth
Specimen A 20-23: embryonic day 9.5 (more )
Note: 20-23 somite pairs. These data are graphed in Figure 3.
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters Expression is in clusters of 10-49 cells.
Specimen A 27-28: embryonic day 9.5 (more )
Note: 27-28 somite pairs. These data are graphed in Figure 3.
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters
Specimen A 35-36: embryonic day 10.5 (more )
Note: 35-36 somite pairs. These data are graphed in Figure 3.
Structure Level Pattern Image Note
TS17: yolk sac Present Clusters
Specimen A 39-40: embryonic day 11.0 (more )
Note: 39-40 somite pairs. These data are graphed in Figure 3.
Structure Level Pattern Image Note
TS18: yolk sac Present Clusters
Specimen A 43-44: embryonic day 11.0 (more )
Note: 43-44 somite pairs. These data are graphed in Figure 3.
Structure Level Pattern Image Note
TS18: yolk sac Present Clusters
Specimen 4A: embryonic day 8.0 (more )
Note: 7 somite pairs. Flat mount. Double labeled: green - Kit; red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS13: yolk sac Present Regionally restricted 4A Expression is in erythro-myeloid progenitors in the proximal region of the yolk sac and interspersed between the primitive erythroblasts.
Specimen 4B: embryonic day 8.75 (more )
Note: 14 somite pairs. Flat mount. Double labeled: green - Kit; red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS14: yolk sac Present Regionally restricted 4B Expression is in erythro-myeloid progenitors in the proximal region of the yolk sac.
Specimen 4B right top: embryonic day 8.75 (more )
Note: 14 somite pairs. Flat mount. Triple labeled: green - Kit; blue - Runx1 (assay MGI:6201389); red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS14: yolk sac Present Regionally restricted 4B right top Expression is in erythro-myeloid progenitors in the proximal region of the yolk sac.
Specimen 4B right bottom: embryonic day 8.75 (more )
Note: 14 somite pairs. Flat mount. Triple labeled: green - Kit; blue - Runx1 (assay MGI:6201389); red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS14: yolk sac Present Regionally restricted 4B right bottom Expression is in erythro-myeloid progenitors in the proximal region of the yolk sac.
Specimen 4C: embryonic day 9.5 (more )
Note: 21 somite pairs. Flat mount. Double labeled: green - Kit; red - Cdh5 (assay MGI:6201390).
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters 4C Expression is in erythro-myeloid progenitor clusters in both the arterial and venous regions of the yolk sac.
Specimen 5A: embryonic day 9.5; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 28 somite pairs. Flat mount. Double labeled: green - Kit; red - Cdh5 (assay MGI:6201390).
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters 5A Expression is in erythro-myeloid progenitors in the proximal region of the yolk sac. Expression in this mutant is the same as in wild-type.
Specimen 5B: embryonic day 9.5; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 23 somite pairs. Flat mount. Triple labeled: green - Kit; blue - Runx1 (assay MGI:6201389); red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 5B Expression is in erythro-myeloid progenitors in the proximal region of the yolk sac. Expression in this mutant is the same as in wild-type.
Specimen 5D: embryonic day 9.5 (more )
Note: 22 somite pairs. Flat mount. Double labeled: green - Kit; red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 5D Expression is in flattened and polygonal cells in larger remodeled vessels and the vascular plexus.
Specimen 5E: embryonic day 9.5 (more )
Note: 29 somite pairs. Flat mount. Double labeled: green - Kit; red - Cdh5 (assay MGI:6201390).
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 5E Expression is in flattened and polygonal cells in larger remodeled vessels and the vascular plexus.
Specimen 5F: embryonic day 9.5 (more )
Note: 20 somite pairs. Flat mount. Triple labeled: green - Kit; red - Kdr (assay MGI:6201396); blue - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 5F Expression is in flattened and polygonal cells in larger remodeled vessels and the vascular plexus.
Specimen S1B Runx1+/-: embryonic day 9.5; Runx1tm1Dow/Runx1+ (more )
Note: 23 somite pairs. Double labeled: green - Kit; red - Kdr (assay MGI:6201396).
Structure Level Pattern Image Note
TS15: yolk sac Present Clusters S1B top
Specimen S1B Runx1-/-: embryonic day 9.5; Runx1tm1Dow/Runx1tm1Dow (more )
Note: 23 somite pairs. Double labeled: green - Kit; red - Kdr (assay MGI:6201396).
Structure Level Pattern Image Note
TS15: yolk sac Absent S1B bottom
Specimen not shown: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: yolk sac Present Clusters Only rare clusters have expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory