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Gene Expression Data
Assay Details
Assay
Reference: J:233088 Frame JM, et al., Definitive Hematopoiesis in the Yolk Sac Emerges from Wnt-Responsive Hemogenic Endothelium Independently of Circulation and Arterial Identity. Stem Cells. 2016 Feb;34(2):431-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6201388
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-CD31
Detection system: Secondary antibody
Assay notes: Fixative was either acetone or 2% formaldehyde.
Results
Specimen 2A: embryonic day 8.5 (more )
Note: 7 somite pairs. Triple labeled: red - Pecam1; green - Kit (assay MGI:6201387); blue - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS13: yolk sac blood vessel Present Regionally restricted 2A Merge , 2A CD31 Expression is in endothelial cells.
Specimen 2B: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: red - Pecam1; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 2B top left Expression is in endothelial cells.
Specimen 2C bottom left: embryonic day 8.0 (more )
Note: 6 somite pairs. Triple labeled: red - Pecam1; green - Kit (assay MGI:6201387); blue - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS12: yolk sac Present 2C bottom left Expression is in endothelial cells.
Specimen 4A: embryonic day 8.0 (more )
Note: 7 somite pairs. Double labeled: red - Pecam1; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS12: yolk sac Present Regionally restricted 4A Expression is in endothelial cells.
Specimen 4B: embryonic day 8.75 (more )
Note: 14 somite pairs. Double labeled: red - Pecam1; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS14: yolk sac blood vessel Present Regionally restricted 4B Expression is in endothelial cells.
Specimen 4B right top: embryonic day 8.75 (more )
Note: 14 somite pairs. Triple labeled: red - Pecam1; green - Kit (assay MGI:6201387), blue - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS14: yolk sac blood vessel Present Regionally restricted 4B right top Expression is in endothelial cells.
Specimen 4B right bottom: embryonic day 8.75 (more )
Note: 14 somite pairs. Triple labeled: red - Pecam1; green - Kit (assay MGI:6201387), blue - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS14: yolk sac blood vessel Present Regionally restricted 4B right bottom Expression is in endothelial cells.
Specimen 5B: embryonic day 9.5; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 23 somite pairs. Flat mount. Triple labeled: red - Pecam1; green - Kit (assay MGI:6201387); blue - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 5B Expression in this mutant is the same as in wild-type.
Specimen 5C: embryonic day 9.5; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 21 somite pairs. Flat mount. Double labeled: red - Pecam1; blue - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 5C
Specimen 5D: embryonic day 9.5 (more )
Note: 22 somite pairs. Flat mount. Double labeled: red - Pecam1; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 5D Expression is in endothelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory