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Gene Expression Data
Assay Details
Assay
Reference: J:233088 Frame JM, et al., Definitive Hematopoiesis in the Yolk Sac Emerges from Wnt-Responsive Hemogenic Endothelium Independently of Circulation and Arterial Identity. Stem Cells. 2016 Feb;34(2):431-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6201389
Gene symbol: Runx1
Gene name: runt related transcription factor 1
Antibody: anti-Runx1
Detection system: Secondary antibody
Assay notes: Fixative was either acetone or 2% formaldehyde.
Results
Specimen 2A: embryonic day 8.5 (more )
Note: 8 somite pairs. Triple labeled: blue- Runx1; green - Kit (assay MGI:6201387); red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS13: yolk sac Present Regionally restricted 2A Runx1 , 2A Merge Expression is in flattened hemogenic endothelial cells and in rounded hematopoietic cells.
Specimen 2B top right: embryonic day 10.0 (more )
Note: 31 somite pairs. Double labeled: red - Runx1; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS16: yolk sac Present Regionally restricted 2B top right Expression is in flattened hemogenic endothelial cells and in rounded hematopoietic cells.
Specimen 2B bottom left: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: red - Runx1; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 2B bottom left Expression is in flattened hemogenic endothelial cells and in rounded hematopoietic cells.
Specimen 2B bottom right: embryonic day 9.5 (more )
Note: 28 somite pairs. Double labeled: red- Runx1; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 2B bottom right Expression is in flattened hemogenic endothelial cells and in rounded hematopoietic cells.
Specimen 2C bottom left: embryonic day 8.0 (more )
Note: 6 somite pairs. Triple labeled: blue - Runx1; green - Kit (assay MGI:6201387); red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS12: yolk sac Present Regionally restricted 2C bottom left Expression is in flattened hemogenic endothelial cells and in rounded hematopoietic cells.
Specimen 2C right: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: red - Runx1; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 2C right Expression is in flattened hemogenic endothelial cells and in rounded hematopoietic cells.
Specimen 4B right top: embryonic day 8.75 (more )
Note: 14 somite pairs. Triple labeled: blue - Runx1; green - Kit (assay MGI:6201387); red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS14: yolk sac Present Clusters 4B right top
Specimen 4B right bottom: embryonic day 8.75 (more )
Note: 14 somite pairs. Triple labeled: blue - Runx1; green - Kit (assay MGI:6201387); red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS14: yolk sac Present Clusters 4B right bottom
Specimen 5B: embryonic day 9.5; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 23 somite pairs. Flat mount. Triple labeled: blue - Runx1; green - Kit (assay MGI:6201387); red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS15: yolk sac Present 5B
Specimen 5C: embryonic day 9.5; Slc8a1tm1Sjc/Slc8a1tm1Sjc (more )
Note: 21 somite pairs. Flat mount. Double labeled: green - Runx1; red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS15: yolk sac Present 5C
Specimen 5F: embryonic day 9.5 (more )
Note: 20 somite pairs. Flat mount. Triple labeled: blue - Runx1; green - Kit (assay MGI:6201387); red - Pecam1 (assay MGI:6201388).
Structure Level Pattern Image Note
TS15: yolk sac Present 5F
Specimen S3B VA: embryonic day 9.5 (more )
Note: 23 somite pairs. Double labeled: red - Runx1; green - Sox17 (assay MGI:6201698).
Structure Level Pattern Image Note
TS15: vitelline artery Present Clusters S3B left
Specimen S3B Arterial: embryonic day 9.5 (more )
Note: 23 somite pairs. Double labeled: red - Runx1; green - Sox17 (assay MGI:6201698).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Clusters S3B middle
Specimen S3B Venous: embryonic day 9.5 (more )
Note: 23 somite pairs. Double labeled: red - Runx1; green - Sox17 (assay MGI:6201698).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Clusters S3B right
Specimen S3C left: embryonic day 9.5 (more )
Note: 23 somite pairs. Double labeled: red - Runx1; green - Sox17 (assay MGI:6201698).
Structure Level Pattern Image Note
TS15: yolk sac Present S3C left
Specimen S3C right: embryonic day 9.5 (more )
Note: 24 somite pairs. Double labeled: red - Runx1; green - Sox17 (assay MGI:6201698).
Structure Level Pattern Image Note
TS15: yolk sac Present S3C left
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory