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Gene Expression Data
Assay Details
Assay
Reference: J:233088 Frame JM, et al., Definitive Hematopoiesis in the Yolk Sac Emerges from Wnt-Responsive Hemogenic Endothelium Independently of Circulation and Arterial Identity. Stem Cells. 2016 Feb;34(2):431-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6201396
Gene symbol: Kdr
Gene name: kinase insert domain protein receptor
Antibody: Flk1 antibody
Detection system: Secondary antibody
Assay notes: Fixative was either acetone or 2% formaldehyde.
Results
Specimen 2D top: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: red - Kdr; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 2D top Expression is in the endothelium.
Specimen 2D second: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: red - Kdr; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 2D second Expression is in the endothelium.
Specimen 2D third: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: red - Kdr; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 2D third Expression is in the endothelium.
Specimen 2D fourth: embryonic day 9.5 (more )
Note: 22 somite pairs. Double labeled: red - Kdr; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 2D fourth Expression is in the endothelium.
Specimen 5F: embryonic day 9.5 (more )
Note: 20 somite pairs. Flat mount. Triple labeled: red - Kdr; green - Kit (assay MGI:6201387); blue - Runx1 (assay MGI:6201389).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted 5F Expression is in the endothelium.
Specimen S1B Runx1+/-: embryonic day 9.5; Runx1tm1Dow/Runx1+ (more )
Note: 23 somite pairs. Double labeled: red - Kdr; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present S1B top Expression is in the endothelium.
Specimen S1B Runx1-/-: embryonic day 9.5; Runx1tm1Dow/Runx1tm1Dow (more )
Note: 23 somite pairs. Double labeled: red - Kdr; green - Kit (assay MGI:6201387).
Structure Level Pattern Image Note
TS15: yolk sac blood vessel Present Regionally restricted S1B bottom Expression is in the endothelium.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory