En2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:57262 Song J, et al., The type II activin receptors are essential for egg cylinder growth, gastrulation, and rostral head development in mice. Dev Biol. 1999 Sep 1;213(1):157-69
Assay type:	RNA in situ
MGI Accession ID:	MGI:5491489
Gene symbol:	En2
Gene name:	engrailed 2

Probe:	En2 cDNA1
Probe preparation:	Antisense labelled with fluorescein RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Expression was detected using INT/BCIP for the color reaction.

Results

4E ©

Specimen 4E: embryonic day 9.5 (more

)
Note: Double labeled: orange - En2; purple - Otx2 (assay MGI:5491488).

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present		4E

4J ©

Specimen 4J: embryonic day 9.5; Acvr2a^tm1Hsch/Acvr2a^tm1Hsch, Acvr2b^tm1Enl/Acvr2b⁺ (more

)
Note: Double labeled: orange - En2; purple - Otx2 (assay MGI:5491488).

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present		4J

4K ©

Specimen 4K: embryonic day 9.5; Acvr2a^tm1Hsch/Acvr2a^tm1Hsch, Acvr2b^tm1Enl/Acvr2b⁺ (more

)
Note: Double labeled: orange - En2; purple - Otx2 (assay MGI:5491488).

Structure	Level	Pattern	Image	Note
TS15: midbrain-hindbrain junction	Present		4K

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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