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En2 Gene Detail
Summary
  • Symbol
    En2
  • Name
    engrailed 2
  • Synonyms
    En-2
  • Feature Type
    protein coding gene
  • IDs
    MGI:95390
    NCBI Gene: 13799
  • Alliance
    genome feature page
  • Transcription Start Sites
    1 TSS
Location &
Maps
more
  • Sequence Map
    Chr5:28370692-28377164 bp, + strand
    From Ensembl annotation of GRCm39
  • View this region in JBrowse
  • Genome Browsers
    Ensembl | UCSC | NCBI
  • Genetic Map
    Chromosome 5, 13.94 cM
  • Mapping Data
    45 experiments
Strain
Comparison
more
  • SNPs within 2kb
    180 from dbSNP Build 142
  • Strain Annotations
    19
  • PCR
    1
  • RFLP
    14
For selected strains:
Strain Gene Model ID Feature Type Coordinates Select Strains
C57BL/6J MGI_C57BL6J_95390
 protein coding gene Chr5:28370692-28377195 (+)
129S1/SvImJ ENSMUSG00200012784
 protein coding gene Chr5:23866307-23872736 (+)
A/J ENSMUSG00195048589
 protein coding gene Chr5:23325799-23332240 (+)
AKR/J ENSMUSG00220007058
 protein coding gene Chr5:23331974-23338419 (+)
BALB/cJ ENSMUSG00180036094
 protein coding gene Chr5:23130462-23136899 (+)
C3H/HeJ ENSMUSG00175043919
 protein coding gene Chr5:25104905-25111344 (+)
C57BL/6NJ ENSMUSG00215024160
 protein coding gene Chr5:23833542-23840019 (+)
CAROLI/EiJ MGP_CAROLIEiJ_G0027037
 protein coding gene Chr5:22802440-22808923 (+)
CAST/EiJ ENSTCUG00005023602
 protein coding gene Chr5:23314677-23321182 (+)
CBA/J ENSMUSG00210035300
 protein coding gene Chr5:24078183-24084626 (+)
DBA/2J ENSMUSG00185035876
 protein coding gene Chr5:25923421-25929886 (+)
FVB/NJ ENSMUSG00205015123
 protein coding gene Chr5:22850853-22857300 (+)
JF1/MsJ ENSUMUG00000051385
 protein coding gene Chr5:30498108-30504619 (+)
LP/J ENSMUSG00230041725
 protein coding gene Chr5:30551657-30558083 (+)
NOD/ShiLtJ ENSMUSG00190011667
 protein coding gene Chr5:23138066-23144509 (+)
NZO/HlLtJ ENSMUSG00225018111
 protein coding gene Chr5:35775655-35782096 (+)
PWK/PhJ ENSLUMG00010047664
 protein coding gene Chr5:23267156-23273639 (+)
SPRET/EiJ ENSMSPG00010032472
 protein coding gene Chr5:24343807-24350168 (+)
WSB/EiJ ENSIUOG00005041097
 protein coding gene Chr5:26007321-26013794 (+)



Homology
more
  • Human Ortholog
    EN2, engrailed homeobox 2
  • Vertebrate Orthologs
    4
Vertebrate Orthology Source
Alliance of Genome Resources
  • Human Ortholog
    EN2, engrailed homeobox 2
  • Links
    NCBI Gene ID: 2020
    UniProt: P19622

  • Chr Location
    7q36.3; chr7:155458129-155464831 (+)  GRCh38
Human Diseases
more
  • Diseases
    1 with En2 mouse models; 1 with human EN2 associations

Human Disease Mouse Models
      
IDs
 View 1 model
      
IDs
Click on a disease name to see all genes associated with that disease.

  • Mutations/Alleles
    1 with disease annotations
  • References
    4 with disease annotations
Mutations,
Alleles, and
Phenotypes
less
  • Phenotype Summary
    31 phenotypes from 6 alleles in 9 genetic backgrounds
    45 phenotypes from multigenic genotypes
    93 phenotype references
Phenotype Overview

adipose tissue
behavior/neurological
cardiovascular system
cellular
craniofacial
digestive/alimentary system
embryo
endocrine/exocrine glands
growth/size/body
hearing/vestibular/ear
hematopoietic system
homeostasis/metabolism
integument
immune system
limbs/digits/tail
liver/biliary system
mortality/aging
muscle
nervous system
pigmentation
renal/urinary system
reproductive system
respiratory system
skeleton
taste/olfaction
neoplasm
vision/eye

Click cells to view annotations.
This locus affects anterior-posterior cerebellar patterning. Homozygous null mutants show altered foliation pattern and perform poorly in motor learning (rotarod) tests. Heterozygotes test intermediate on rotarod. Hypomorphs show no phenotypic effects.
Gene Ontology
(GO)
Classifications
less
  • All GO Annotations
    31
  • GO References
    15
Molecular Function

carbohydrate derivative binding
cytoskeletal protein binding
DNA binding
enzyme regulator
hydrolase
ligase
lipid binding
oxidoreductase
RNA binding
signaling receptor activity
signaling receptor binding
transcription
transferase
transporter
Biological Process

carbohydrate derivative metabolism
cell differentiation
cell population proliferation
cellular component organization
DNA-templated transcription
establishment of localization
homeostatic process
immune system process
lipid metabolic process
programmed cell death
protein metabolic process
response to stimulus
signaling
system development
Cellular Component

cell projection
cytoplasmic vesicle
cytoskeleton
cytosol
endoplasmic reticulum
endosome
extracellular region
Golgi apparatus
mitochondrion
membraneless organelle
nucleus
organelle envelope
organelle lumen
plasma membrane
protein-containing complex
synapse
vacuole
Click cells to view annotations.
Expression
less
Expression Overview

early conceptus
embryo ectoderm
embryo endoderm
embryo mesoderm
embryo mesenchyme
extraembryonic component
alimentary system
auditory system
branchial arches
cardiovascular system
connective tissue
endocrine system
exocrine system
hemolymphoid system
integumental system
limbs
liver and biliary system
musculoskeletal system
nervous system
olfactory system
reproductive system
respiratory system
urinary system
visual system
Click cells to view annotations.


  • Assay Results
    1416
  • Tissues
    285
  • cDNA Data
    22
  • Literature Summary
    163
  • Comparison Matrix
    Gene Expression + Phenotype
    • Sequences &
    Gene Models
    less
    Representative SequencesLengthStrain/SpeciesFlank
    genomic ENSMUSG00000039095 Ensembl Gene Model | MGI Sequence Detail 6473 C57BL/6J ±  kb
    transcript ENSMUST00000036177 Ensembl | MGI Sequence Detail 3539 Not Applicable  
    polypeptide ENSMUSP00000036761 Ensembl | MGI Sequence Detail 324 Not Applicable  
    For the selected sequence
    Protein
    Information
    less
    Molecular
    Reagents
    less
    • All nucleic 67
      Genomic 6
      cDNA 25
      Primer pair 11
      Other 25
      Antibodies 4

      Microarray probesets 3
    Other
    Accession IDs
    less
    MGD-MRK-9498, MGD-MRK-9500, MGI:2141264
    References
    more
    • Summaries
      All 326
      Developmental Gene Expression 163
      Diseases 4
      Gene Ontology 15
      Phenotypes 93
    • Earliest
      J:5021 Batchelor AL, et al., A comparison of the mutagenic effectiveness of chronic neutron- and gamma-irradiation of mouse spermatogonia. Mutat Res. 1966 Jun;3(3):218-29
    • Latest
      J:360569 Trinh LT, et al., Positive autoregulation of Sox17 is necessary for gallbladder and extrahepatic biliary duct formation. Development. 2025;:dev.203033
    Contributing Projects:
    Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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    Funding Information
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    Send questions and comments to User Support.     		last database update
    09/08/2026
    MGI 6.24     		The Jackson Laboratory