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Gene Expression Data
Assay Details
Assay
Reference: J:118937 Kaji K, et al., Mbd3, a component of the NuRD co-repressor complex, is required for development of pluripotent cells. Development. 2007 Mar;134(6):1123-32
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3703637
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4 (N-19; sc-8628)
Detection system: Secondary antibody
Assay notes: The authors did not specify which antibody was used in this assay, a different anti-Oct4 antibody (MGI:3702926) could have been used.
Results
Specimen 2A +/+: embryonic day 3.5 (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present 2A +/+
Specimen 2A +/-: embryonic day 3.5; Mbd3tm1Bh/Mbd3+ (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present 2A +/-
Specimen 2A -/-: embryonic day 3.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Structure Level Pattern Image Note
TS5: inner cell mass Present 2A -/- Expression in this mutant is the same as in wild-type embryos.
Specimen 2B +/-: embryonic day 4.5; Mbd3tm1Bh/Mbd3+ (more )
Note: Double labeled: green - Pou5f1; red - Gata4 (assay MGI:3703639).
Structure Level Pattern Image Note
TS6: embryo Present 2B +/- Oct4/Gata4
Specimen 2B -/-: embryonic day 4.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Note: Double labeled: green - Pou5f1; red - Gata4 (assay MGI:3703639).
Structure Level Pattern Image Note
TS6: embryo Present 2B -/- Oct4/Gata4 Expression in this mutant is the same as in wild-type embryos.
Specimen 2C 5.5dpc +/-: embryonic day 5.5; Mbd3tm1Bh/Mbd3+ (more )
Structure Level Pattern Image Note
TS8: embryo Present 2C 5.5 +/-
Specimen 2C 5.5dpc -/-: embryonic day 5.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Structure Level Pattern Image Note
TS8: embryo Present 2C 5.5 -/- Expression in this mutant is the same as in wild-type embryos.
Specimen 2C 6.5dpc +/-: embryonic day 6.5; Mbd3tm1Bh/Mbd3+ (more )
Structure Level Pattern Image Note
TS9: embryo Present 2C 6.5 +/-
Specimen 2C 6.5dpc -/-: embryonic day 6.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Structure Level Pattern Image Note
TS9: embryo Weak 2C 6.5 -/-
Specimen 2C 7.5dpc +/-: embryonic day 7.5; Mbd3tm1Bh/Mbd3+ (more )
Structure Level Pattern Image Note
TS11: embryo Present 2C 7.5 +/-
Specimen 2C 7.5dpc -/-: embryonic day 7.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Structure Level Pattern Image Note
TS11: embryo Weak 2C 7.5 -/-
Specimen 2C 8.5dpc +/-: embryonic day 8.5; Mbd3tm1Bh/Mbd3+ (more )
Structure Level Pattern Image Note
TS13: embryo Present 2C 8.5 +/-
Specimen 2C 8.5dpc -/-: embryonic day 8.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Structure Level Pattern Image Note
TS13: embryo Absent 2C 8.5 -/-
TS13: extraembryonic component Present Regionally restricted 2C 8.5 -/- Expression is in the ectoplacental cone.
Specimen 3A +/+: embryonic day 5.5 (more )
Note: Double labeled: green - Pou5f1; red - Itga7 (assay MGI:3703713).
Structure Level Pattern Image Note
TS8: embryo Present 3A Oct4 +/+ , 3A merge +/+
Specimen 3A -/-: embryonic day 5.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Note: Double labeled: green - Pou5f1; red - Itga7 (assay MGI:3703713).
Structure Level Pattern Image Note
TS8: embryo Present 3A merge -/- , 3A Oct4 -/-
Specimen 3C +/-: embryonic day 5.5; Mbd3tm1Bh/Mbd3+ (more )
Note: Double labeled: green - Pou5f1; red - Gata4 (assay MGI:3703639).
Structure Level Pattern Image Note
TS8: embryo Present 3C Oct4 +/- , 3C merge +/- Higher magnification was shown in the inset.
Specimen 3C -/-: embryonic day 5.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Note: Double labeled: green - Pou5f1; red - Gata4 (assay MGI:3703639).
Structure Level Pattern Image Note
TS8: embryo Present 3C Oct4 -/- , 3C merge -/- Higher magnification was shown in the inset.
Specimen S1A +/+: embryonic day 5.5 (more )
Note: Double labeled: green - Pou5f1; red - activated Casp3 (assay MGI:3703707).
Structure Level Pattern Image Note
TS8: embryo Present S1A +/+
Specimen S1A +/-: embryonic day 5.5; Mbd3tm1Bh/Mbd3+ (more )
Note: Double labeled: green - Pou5f1; red - activated Casp3 (assay MGI:3703707).
Structure Level Pattern Image Note
TS8: embryo Present S1A +/-
Specimen S1A -/- left: embryonic day 5.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Note: Double labeled: green - Pou5f1; red - activated Casp3 (assay MGI:3703707).
Structure Level Pattern Image Note
TS8: embryo Present S1A -/- left Expression in this mutant is the same as in wild-type embryos.
Specimen S1A -/- right: embryonic day 5.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Note: Double labeled: green - Pou5f1; red - activated Casp3 (assay MGI:3703707).
Structure Level Pattern Image Note
TS8: embryo Present S1A -/- right Expression in this mutant is the same as in wild-type embryos.
Specimen S1B +/+: embryonic day 5.5 (more )
Note: Double labeled: green - Pou5f1; red - Mki67 (assay MGI:3703710).
Structure Level Pattern Image Note
TS8: embryo Present S1B +/+
TS8: extraembryonic component Present S1B +/+
Specimen S1B -/-: embryonic day 5.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Note: Double labeled: green - Pou5f1; red - Mki67 (assay MGI:3703710).
Structure Level Pattern Image Note
TS8: embryo Present S1B -/- Expression in this mutant is the same as in wild-type embryos.
TS8: extraembryonic component Present S1B -/- Expression in this mutant is the same as in wild-type embryos.
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03/25/2025
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