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Gene Expression Data
Assay Details
Assay
Reference: J:118937 Kaji K, et al., Mbd3, a component of the NuRD co-repressor complex, is required for development of pluripotent cells. Development. 2007 Mar;134(6):1123-32
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3703639
Gene symbol: Gata4
Gene name: GATA binding protein 4
Antibody: anti-Gata4
Detection system: Secondary antibody
Results
Specimen 2B +/- Oct4/Gata4: embryonic day 4.5; Mbd3tm1Bh/Mbd3+ (more )
Note: Double labeled: red - Gata4; green - Pou5f1 (assay MGI:3703637).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 2B +/- Oct4/Gata4
Specimen 2B -/- Oct4/Gata4: embryonic day 4.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Note: Double labeled: red - Gata4; green - Pou5f1 (assay MGI:3703637).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 2B -/- Oct4/Gata4 Expression in this mutant is the same as in wild-type embryos.
Specimen 2B +/- Nanog/Gata4: embryonic day 4.5; Mbd3tm1Bh/Mbd3+ (more )
Note: Double labeled: red - Gata4; green - Nanog (assay MGI:3703641).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 2B +/- Nanog/Gata4
Specimen 2B -/- Nanog/Gata4: embryonic day 4.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Note: Double labeled: red - Gata4; green - Nanog (assay MGI:3703641).
Structure Level Pattern Image Note
TS6: primitive endoderm Present 2B -/- Nanog/Gata4 Expression in this mutant is the same as in wild-type embryos.
Specimen 3C +/-: embryonic day 5.5; Mbd3tm1Bh/Mbd3+ (more )
Note: Double labeled: red - Gata4; green - Pou5f1 (assay MGI:3703637).
Structure Level Pattern Image Note
TS8: embryo Present 3C Gata4 +/- , 3C merge +/- Higher magnification was shown in the inset.
Specimen 3C -/-: embryonic day 5.5; Mbd3tm1Bh/Mbd3tm1Bh (more )
Note: Double labeled: red - Gata4; green - Pou5f1 (assay MGI:3703637).
Structure Level Pattern Image Note
TS8: embryo Present 3C Gata4 -/- , 3C merge -/- Higher magnification was shown in the inset.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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