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Spag16 Gene Detail
Summary
  • Symbol
    Spag16
  • Name
    sperm associated antigen 16
  • Synonyms
    4921511D23Rik, 4930524F24Rik, 4930585K05Rik, Pf20, Wdr29
  • Feature Type
    protein coding gene
  • IDs
    MGI:1913972
    NCBI Gene: 66722
  • Alliance
    genome feature page
  • Transcription Start Sites
    10 TSS
Location &
Maps
more
  • Sequence Map
    Chr1:69866129-70764291 bp, + strand
    From Ensembl annotation of GRCm39
  • View this region in JBrowse
  • Genome Browsers
    Ensembl | UCSC | NCBI
  • Genetic Map
    Chromosome 1, 35.04 cM, cytoband C3
  • Mapping Data
    4 experiments
Strain
Comparison
more
  • SNPs within 2kb
    25871 from dbSNP Build 142
  • Strain Annotations
    19
For selected strains:
Strain Gene Model ID Feature Type Coordinates Select Strains
C57BL/6J MGI_C57BL6J_1913972
 protein coding gene Chr1:69866031-70764291 (+)
129S1/SvImJ ENSMUSG00200004443
 protein coding gene Chr1:67519780-68422356 (+)
A/J ENSMUSG00195030772
 protein coding gene Chr1:66943099-67836221 (+)
AKR/J ENSMUSG00220036991
 protein coding gene Chr1:67104205-68011261 (+)
BALB/cJ ENSMUSG00180028625
 protein coding gene Chr1:67202076-68104661 (+)
C3H/HeJ ENSMUSG00175000757
 protein coding gene Chr1:67329377-68232012 (+)
C57BL/6NJ ENSMUSG00215015562
 protein coding gene Chr1:67240470-68144891 (+)
CAROLI/EiJ MGP_CAROLIEiJ_G0014234
 protein coding gene Chr1:63472678-64344631 (+)
CAST/EiJ ENSTCUG00005020728
 protein coding gene Chr1:66990027-67890465 (+)
CBA/J ENSMUSG00210003062
 protein coding gene Chr1:67030153-67932711 (+)
DBA/2J ENSMUSG00185004890
 protein coding gene Chr1:68120686-69023263 (+)
FVB/NJ ENSMUSG00205012313
 protein coding gene Chr1:66785986-67676969 (+)
JF1/MsJ ENSUMUG00000002380
 protein coding gene Chr1:67039072-67903254 (+)
LP/J ENSMUSG00230001514
 protein coding gene Chr1:68115029-69017590 (+)
NOD/ShiLtJ ENSMUSG00190012820
 protein coding gene Chr1:67238208-68148710 (+)
NZO/HlLtJ ENSMUSG00225000890
 protein coding gene Chr1:71330114-72232664 (+)
PWK/PhJ ENSLUMG00010007144
 protein coding gene Chr1:66542156-67456615 (+)
SPRET/EiJ ENSMSPG00010017161
 protein coding gene Chr1:68649382-69583362 (+)
WSB/EiJ ENSIUOG00005028456
 protein coding gene Chr1:66846420-67765661 (+)



Homology
more
  • Human Ortholog
    SPAG16, sperm associated antigen 16
  • Vertebrate Orthologs
    2
Vertebrate Orthology Source
Alliance of Genome Resources
  • Human Ortholog
    SPAG16, sperm associated antigen 16
  • Synonyms
    PF20, WDR29
  • Links
    NCBI Gene ID: 79582
    UniProt: Q8N0X2

  • Chr Location
    2q34; chr2:213284389-214410501 (+)  GRCh38
Mutations,
Alleles, and
Phenotypes
less
  • Phenotype Summary
    16 phenotypes from 2 alleles in 2 genetic backgrounds
    18 phenotype references
Phenotype Overview

adipose tissue
behavior/neurological
cardiovascular system
cellular
craniofacial
digestive/alimentary system
embryo
endocrine/exocrine glands
growth/size/body
hearing/vestibular/ear
hematopoietic system
homeostasis/metabolism
integument
immune system
limbs/digits/tail
liver/biliary system
mortality/aging
muscle
nervous system
pigmentation
renal/urinary system
reproductive system
respiratory system
skeleton
taste/olfaction
neoplasm
vision/eye

Click cells to view annotations.
Chimeric males carrying one copy of the mutated allele have impaired spermatogenesis, a significant loss of germ cells at the round spermatid stage, and disorganized sperm axoneme structure. No offspring carrying the mutated allele are produced from matings using male chimeras.
Gene Ontology
(GO)
Classifications
less
  • All GO Annotations
    29
  • GO References
    16
Molecular Function

carbohydrate derivative binding
cytoskeletal protein binding
DNA binding
enzyme regulator
hydrolase
ligase
lipid binding
oxidoreductase
RNA binding
signaling receptor activity
signaling receptor binding
transcription
transferase
transporter
Biological Process

carbohydrate derivative metabolism
cell differentiation
cell population proliferation
cellular component organization
DNA-templated transcription
establishment of localization
homeostatic process
immune system process
lipid metabolic process
programmed cell death
protein metabolic process
response to stimulus
signaling
system development
Cellular Component

cell projection
cytoplasmic vesicle
cytoskeleton
cytosol
endoplasmic reticulum
endosome
extracellular region
Golgi apparatus
mitochondrion
membraneless organelle
nucleus
organelle envelope
organelle lumen
plasma membrane
protein-containing complex
synapse
vacuole
Click cells to view annotations.
Expression
less
Expression Overview

early conceptus
embryo ectoderm
embryo endoderm
embryo mesoderm
embryo mesenchyme
extraembryonic component
alimentary system
auditory system
branchial arches
cardiovascular system
connective tissue
endocrine system
exocrine system
hemolymphoid system
integumental system
limbs
liver and biliary system
musculoskeletal system
nervous system
olfactory system
reproductive system
respiratory system
urinary system
visual system
Click cells to view annotations.


  • Assay Results
    597
  • cDNA Data
    17
  • Literature Summary
    1
  • Comparison Matrix
    Gene Expression + Phenotype
    • Sequences &
    Gene Models
    less
    Representative SequencesLengthStrain/SpeciesFlank
    genomic ENSMUSG00000053153 Ensembl Gene Model | MGI Sequence Detail 898163 C57BL/6J ±  kb
    transcript ENSMUST00000065425 Ensembl | MGI Sequence Detail 2126 Not Applicable  
    polypeptide ENSMUSP00000069821 Ensembl | MGI Sequence Detail 639 Not Applicable  
    For the selected sequence
    Protein
    Information
    less
    Molecular
    Reagents
    less
    • All nucleic 18
      cDNA 17
      Primer pair 1

      Microarray probesets 5
    Other
    Accession IDs
    less
    MGI:1922324, MGI:1923198, MGI:2138527
    References
    more
    • Summaries
      All 53
      Developmental Gene Expression 1
      Gene Ontology 16
      Phenotypes 18
    • Earliest
      J:134667 Roderick TH, et al., Two radiation-induced chromosomal inversions in mice (Mus musculus). Proc Natl Acad Sci U S A. 1970 Oct;67(2):961-7
    • Latest
      J:376101 Chen J, et al., CFAP65 is essential for C2a projection integrity in axonemes: implications for organ-specific ciliary dysfunction and infertility. Cell Mol Life Sci. 2025 Jan 24;82(1):61
    Contributing Projects:
    Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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    Funding Information
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    Send questions and comments to User Support.     		last database update
    09/01/2026
    MGI 6.24     		The Jackson Laboratory