Phenotypes associated with this allele

• Allele Symbol: Sele^tm2Alb
• Allele Name: targeted mutation 2, Arthur L Beaudet
• Allele ID: MGI:3767067
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Sele^tm2Alb/Sele^tm2Alb Sell^tm2Alb/Sell^tm2Alb Selp^tm1Bay/Selp^tm1Bay	involves: 129S7/SvEvBrd * C57BL/6	MGI:2656301
cx2	Icam1^tm1Bay/Icam1^tm1Bay Sele^tm2Alb/Sele^tm2Alb Selp^tm1Bay/Selp^tm1Bay	involves: 129S7/SvEvBrd * C57BL/6	MGI:3766947
cx3	Sele^tm2Alb/Sele^tm2Alb Selp^tm1Bay/Selp^tm1Bay	involves: 129S7/SvEvBrd * C57BL/6	MGI:3766950
cx4	Sele^tm2Alb/Sele^tm2Alb Selp^tm1Bay/Selp^tm1Bay	involves: 129S7/SvEvBrd * C57BL/6J	MGI:3606634

Genotype
MGI:2656301

cx1

• Allelic Composition: Sele^tm2Alb/Sele^tm2Alb; Sell^tm2Alb/Sell^tm2Alb; Selp^tm1Bay/Selp^tm1Bay
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:70682 )

N • GM-CSF levels are comparable to levels in wild-type or Sell-null mutants; elevation observed in Sele/Selp double null mice is absent

abnormal cellular extravasation ( J:70682 )

• neutrophil influx to the peritoneal cavity during thioglycolate-induced peritonitis is significantly reduced compared to wild-type animals in this model

impaired leukocyte tethering or rolling ( J:70682 )

• rolling is severely compromised; no rolling is seen up to 5 hours after cremaster muscle exteriorization

• rolling flux and rolling fraction are more reduced than the decrease observed in Sele/Selp double null mice

increased leukocyte cell number ( J:70682 )

• white blood cell counts (total, eosinophil, neutrophil, monocyte counts) are significantly elevated compared to wild-type, but are ~half the value found in Sele/Selp double mutants

increased eosinophil cell number ( J:70682 )

increased neutrophil cell number ( J:70682 , J:106550 )

• in liver parenchyma and sinusoids, neutrophil numbers are ~2-fold higher in mutants after Gal/ET treatment; numbers of neutrophils in liver tissue are significantly elevated 4 hours prior to Gal/ET treatment, compared to controls (J:106550)

• more neutrophils accumulate in the hepatic vascular beds (sinusoids and venules) after Gal/ET treatment in mutants than in wild-type controls (J:106550)

increased monocyte cell number ( J:70682 )

abnormal spleen morphology ( J:70682 )

• moderate disruption of splenic architecture is observed

abnormal spleen B cell follicle morphology ( J:70682 )

• lymphoid follicles of the white pulp are identified occasionally and have irregular shapes and sizes

decreased spleen B cell follicle number ( J:106550 )

abnormal spleen B cell follicle shape ( J:106550 )

• irregular

enlarged lymph nodes ( J:70682 )

• both old and young mice show severe cervical lymphadenopathy, compared to wild-type or Sell-null mice; condition is more severe than in Sele/Selp/Sell-triple null mice

abnormal cervical lymph node morphology ( J:70682 )

• both old and young mice show marked cervical lymphadenopathy, compared to wild-type or Sell-null mice; condition is less severe than in Sele/Sell-double null mice

lung inflammation ( J:70682 )

• both old and younger mice show bronchial lymphoid aggregates

hematopoietic system

abnormal cellular extravasation ( J:70682 )

• neutrophil influx to the peritoneal cavity during thioglycolate-induced peritonitis is significantly reduced compared to wild-type animals in this model

impaired leukocyte tethering or rolling ( J:70682 )

• rolling is severely compromised; no rolling is seen up to 5 hours after cremaster muscle exteriorization

• rolling flux and rolling fraction are more reduced than the decrease observed in Sele/Selp double null mice

extramedullary hematopoiesis ( J:70682 )

• mice show moderate extramedullary hematopoiesis in sinusoid of red pulp

increased leukocyte cell number ( J:70682 )

• white blood cell counts (total, eosinophil, neutrophil, monocyte counts) are significantly elevated compared to wild-type, but are ~half the value found in Sele/Selp double mutants

increased eosinophil cell number ( J:70682 )

increased neutrophil cell number ( J:70682 , J:106550 )

• in liver parenchyma and sinusoids, neutrophil numbers are ~2-fold higher in mutants after Gal/ET treatment; numbers of neutrophils in liver tissue are significantly elevated 4 hours prior to Gal/ET treatment, compared to controls (J:106550)

• more neutrophils accumulate in the hepatic vascular beds (sinusoids and venules) after Gal/ET treatment in mutants than in wild-type controls (J:106550)

increased monocyte cell number ( J:70682 )

abnormal spleen morphology ( J:70682 )

• moderate disruption of splenic architecture is observed

abnormal spleen B cell follicle morphology ( J:70682 )

• lymphoid follicles of the white pulp are identified occasionally and have irregular shapes and sizes

decreased spleen B cell follicle number ( J:106550 )

abnormal spleen B cell follicle shape ( J:106550 )

• irregular

respiratory system

lung inflammation ( J:70682 )

• both old and younger mice show bronchial lymphoid aggregates

abnormal lung morphology ( J:70682 )

• lung tissue displays decreasing cellularity within alveolocapillary walls, but this is less severe than in Sele/Selp double-nulls

abnormal lung vasculature morphology ( J:70682 )

• only a small increase in leukocytes in alveolocapillary walls are observed and occasional small bronchial lymphoid aggregates are found

cardiovascular system

abnormal lung vasculature morphology ( J:70682 )

• only a small increase in leukocytes in alveolocapillary walls are observed and occasional small bronchial lymphoid aggregates are found

liver/biliary system

hepatic necrosis ( J:106550 )

• at 7 hours after galactosamine/bacterial endotoxin (Gal/ET) treatment, area on necrosis is attenuated 68% compared to treated controls

abnormal liver physiology ( J:106550 )

• mutants show decreased liver injury relative to treated controls at 7 hours after treatment with Gal/ET

homeostasis/metabolism

decreased circulating alanine transaminase level ( J:106550 )

• at 7 hours after galactosamine/bacterial endotoxin (Gal/ET) treatment, plasma ALT levels are reduced by 65% compared to treated controls

integument

integument phenotype ( J:70682 )

N • mice do not show skin ulcerations as seen in double Sele/Selp mutants

cellular

hepatic necrosis ( J:106550 )

• at 7 hours after galactosamine/bacterial endotoxin (Gal/ET) treatment, area on necrosis is attenuated 68% compared to treated controls

abnormal cellular extravasation ( J:70682 )

• neutrophil influx to the peritoneal cavity during thioglycolate-induced peritonitis is significantly reduced compared to wild-type animals in this model

impaired leukocyte tethering or rolling ( J:70682 )

• rolling is severely compromised; no rolling is seen up to 5 hours after cremaster muscle exteriorization

• rolling flux and rolling fraction are more reduced than the decrease observed in Sele/Selp double null mice

Genotype
MGI:3766947

cx2

• Allelic Composition: Icam1^tm1Bay/Icam1^tm1Bay; Sele^tm2Alb/Sele^tm2Alb; Selp^tm1Bay/Selp^tm1Bay
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

immune system

impaired neutrophil chemotaxis ( J:112286 )

• migration is completely defective in acute croton oil dermatitis model in young mice (7-14 weeks); older mice show complete abrogation of neutrophil migration in response to irritation

increased neutrophil cell number ( J:112286 )

• circulating neutrophils are increased in mice with acute croton oil dermatitis

decreased acute inflammation ( J:112286 )

• in response to croton oil application to the ear in young mice (7-14 weeks), inflammation is significantly reduced relative to controls

hematopoietic system

impaired neutrophil chemotaxis ( J:112286 )

• migration is completely defective in acute croton oil dermatitis model in young mice (7-14 weeks); older mice show complete abrogation of neutrophil migration in response to irritation

increased neutrophil cell number ( J:112286 )

• circulating neutrophils are increased in mice with acute croton oil dermatitis

homeostasis/metabolism

skin edema ( J:112286 )

• dermal edema is decreased by 41% within 6 hours of croton oil irritation in young mice (7-14 weeks), and partial suppression of edema in older (23-35 weeks) mice

integument

integument phenotype ( J:112286 )

N • triply deficient mice do not develop spontaneous skin lesions up to 55 weeks of age

skin edema ( J:112286 )

• dermal edema is decreased by 41% within 6 hours of croton oil irritation in young mice (7-14 weeks), and partial suppression of edema in older (23-35 weeks) mice

cellular

impaired neutrophil chemotaxis ( J:112286 )

• migration is completely defective in acute croton oil dermatitis model in young mice (7-14 weeks); older mice show complete abrogation of neutrophil migration in response to irritation

Genotype
MGI:3766950

cx3

• Allelic Composition: Sele^tm2Alb/Sele^tm2Alb; Selp^tm1Bay/Selp^tm1Bay
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6

immune system

impaired leukocyte tethering or rolling ( J:70682 )

• rolling is severely compromised

• rolling flux and rolling fraction are reduced relative to wild-type

increased leukocyte cell number ( J:70682 )

• white blood cell counts (total, eosinophil, neutrophil, monocyte counts) are significantly elevated compared to wild-type

increased eosinophil cell number ( J:70682 )

increased neutrophil cell number ( J:70682 , J:112286 )

• circulating neutrophils are increased in mutants and this increases with age of mice (J:112286)

• circulating neutrophils are increased to a greater extent in older mutants showing spontaneous lesions than in those without spontaneous lesions (J:112286)

increased monocyte cell number ( J:70682 )

abnormal spleen B cell follicle morphology ( J:70682 )

• lymphoid follicles forming the white pulp are effaced and infrequently observed due to expansion of the red pulp by extramedullary hematopoiesis

decreased spleen B cell follicle number ( J:70682 )

abnormal neutrophil physiology ( J:112286 )

• migration is defective in young mice (7-14 weeks) in acute croton oil dermatitis model

• in older mutants (23-35 weeks), neutrophil emigration during irritant dermatitis shows no compromise compared to wild-type

• in young mice (8 weeks) with croton oil-induced lesions on their backs 15 days before acute croton oil-induced dermatitis on the ear, a 5-fold greater emigration of neutrophils to the ear irritation is observed compared to mutants without experimental lesions on their backs, while circulating neutrophil numbers are similar for both groups

abnormal lymph node secondary follicle morphology ( J:70682 )

• structures aren't recognized in mutant cervical lymph nodes

abnormal lymph node germinal center morphology ( J:70682 )

• structures aren't recognized in mutant cervical lymph nodes

enlarged lymph nodes ( J:70682 )

• both old and young mice show severe cervical lymphadenopathy, compared to wild-type or Sell-null mice; condition is more severe than in Sele/Selp/Sell-triple null mice

abnormal cytokine secretion ( J:70682 )

• GM-CSF levels are elevated 6-7-fold above levels in wild-type or Sell-null mice

decreased acute inflammation ( J:112286 )

• standardized volume fraction of emigrated neutrophils in dermis of irritated ears is 0.017 +/- 0.006, compared to 0.317 in irritated ears of wild-type mice

hematopoietic system

impaired leukocyte tethering or rolling ( J:70682 )

• rolling is severely compromised

• rolling flux and rolling fraction are reduced relative to wild-type

extramedullary hematopoiesis ( J:70682 )

• mice show massive extramedullary hematopoiesis with identifiable myeloid and megakaryocytic lineages, and lesser erythroid component

increased leukocyte cell number ( J:70682 )

• white blood cell counts (total, eosinophil, neutrophil, monocyte counts) are significantly elevated compared to wild-type

increased eosinophil cell number ( J:70682 )

increased neutrophil cell number ( J:70682 , J:112286 )

• circulating neutrophils are increased in mutants and this increases with age of mice (J:112286)

• circulating neutrophils are increased to a greater extent in older mutants showing spontaneous lesions than in those without spontaneous lesions (J:112286)

increased monocyte cell number ( J:70682 )

abnormal spleen B cell follicle morphology ( J:70682 )

• lymphoid follicles forming the white pulp are effaced and infrequently observed due to expansion of the red pulp by extramedullary hematopoiesis

decreased spleen B cell follicle number ( J:70682 )

abnormal neutrophil physiology ( J:112286 )

• migration is defective in young mice (7-14 weeks) in acute croton oil dermatitis model

• in older mutants (23-35 weeks), neutrophil emigration during irritant dermatitis shows no compromise compared to wild-type

• in young mice (8 weeks) with croton oil-induced lesions on their backs 15 days before acute croton oil-induced dermatitis on the ear, a 5-fold greater emigration of neutrophils to the ear irritation is observed compared to mutants without experimental lesions on their backs, while circulating neutrophil numbers are similar for both groups

cardiovascular system

abnormal lung vasculature morphology ( J:70682 )

• in mutants, alveolocapillary walls are engorged with leukocytes; these leukocytes are confined to small vascular spaces and do not infiltrate the interstitial supporting tissue or alveolar and airway spaces

respiratory system

abnormal lung morphology ( J:70682 )

• lung tissue displays decreasing cellularity within alveolocapillary walls with severely affected interstitial areas

abnormal lung vasculature morphology ( J:70682 )

• in mutants, alveolocapillary walls are engorged with leukocytes; these leukocytes are confined to small vascular spaces and do not infiltrate the interstitial supporting tissue or alveolar and airway spaces

integument

spontaneous skin ulceration ( J:70682 , J:112286 )

• mice develop mucocutaneous infections or ulcerative dermatitis (J:70682)

• at 15 months, mice show large areas of ulceration on the neck (J:70682)

• mice develop spontaneous skin lesions characterized by polymorphonuclear leukocytes (J:112286)

cellular

impaired leukocyte tethering or rolling ( J:70682 )

• rolling is severely compromised

• rolling flux and rolling fraction are reduced relative to wild-type

Genotype
MGI:3606634

cx4

• Allelic Composition: Sele^tm2Alb/Sele^tm2Alb; Selp^tm1Bay/Selp^tm1Bay
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J

immune system

abnormal leukocyte cell number ( J:101979 )

• elevated peripheral leukocyte count (3 fold) relative to controls prior to and following infection

• decreased peritoneal WBC count relative to peripheral WBC count following infection

increased neutrophil cell number ( J:101979 )

• neutrophilia in contrast to controls following infection with S. pneumoniae

• decreased number of neutrophils in peritoneal fluid relative to peripheral absolute neutrophil counts following infection

eye inflammation ( J:101979 )

• panophthalmitis observed in 8% of mice following infection with S. pneumoniae

increased susceptibility to bacterial infection ( J:101979 )

• severity and duration of morbidity higher than wild-type after infection with Streptococcus pneumoniae

• decreased survival rate after post-infection day 5

• decreased ability to clear bacteremia among survivors

behavior/neurological

abnormal pilomotor reflex ( J:101979 )

• observed following infection with S. pneumoniae

tremors ( J:101979 )

• observed following infection with S. pneumoniae

head tilt ( J:101979 )

• observed following infection with S. pneumoniae

decreased locomotor activity ( J:101979 )

• observed following infection with S. pneumoniae

spinning ( J:101979 )

• observed following infection with S. pneumoniae

growth/size/body

weight loss ( J:101979 )

• 48 hours post-infection with S. pneumoniae

vision/eye

eye inflammation ( J:101979 )

• panophthalmitis observed in 8% of mice following infection with S. pneumoniae

hematopoietic system

abnormal leukocyte cell number ( J:101979 )

• elevated peripheral leukocyte count (3 fold) relative to controls prior to and following infection

• decreased peritoneal WBC count relative to peripheral WBC count following infection

increased neutrophil cell number ( J:101979 )

• neutrophilia in contrast to controls following infection with S. pneumoniae

• decreased number of neutrophils in peritoneal fluid relative to peripheral absolute neutrophil counts following infection