Icam1^tm1Bay
Targeted Allele Detail

Summary

• Symbol: Icam1^tm1Bay
• Name: intercellular adhesion molecule 1; targeted mutation 1, Baylor College of Medicine
• MGI ID: MGI:1857183
• Synonyms: CD54^-, ICAM-1-
• Gene: Icam1 Location: Chr9:20927281-20940113 bp, + strand Genetic Position: Chr9, 7.69 cM
• Alliance: Icam1^tm1Bay page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:14565
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into exon 5 of the gene. Subsequent analysis reported by King, et. al. (J:25530) revealed the presence of alternatively spliced transcripts in both wild-type and homozygous mutant mice. Protein expression was detected in mutant mice in medullary stromal cells of the thymus, as well as in the lung and spleen. Variable splicing of exons 2 through 6, encoding extracellular Ig domains, results in the production of 3 isoforms (2-6, 3-6, 4-6, where the numbers refer to spliced exons) in mutant mice. In addition to the 3 found in mutant mice, 2 other alternative splice forms including exon 5 were identified in wild-type mice. The reading frame and exons 1 and 7, encoding the signal sequence and both the transmembrane and cytosolic domains, are left intact in each isoform. (J:14565, J:25530)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Icam1 Mutation: 24 strains or lines available

References

• Original: J:14565 Sligh JE Jr, et al., Inflammatory and immune responses are impaired in mice deficient in intercellular adhesion molecule 1. Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8529-33
• All: 121 reference(s)