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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Il27ratm1Mak
targeted mutation 1, Tak Mak
MGI:3040914
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Il27ratm1Mak/Il27ratm1Mak B6.129P2-Il27ratm1Mak MGI:4943677
hm2
Il27ratm1Mak/Il27ratm1Mak involves: 129P2/OlaHsd * C57BL/6 MGI:4943708
cx3
Il10tm1Cgn/Il10tm1Cgn
Il27ratm1Mak/Il27ratm1Mak
involves: 129P2/OlaHsd * C57BL/6 MGI:4943707
cx4
Faslpr/Faslpr
Il27ratm1Mak/Il27ratm1Mak
MRL.Cg-Il27ratm1Mak Faslpr MGI:4943704


Genotype
MGI:4943677
hm1
Allelic
Composition
Il27ratm1Mak/Il27ratm1Mak
Genetic
Background
B6.129P2-Il27ratm1Mak
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il27ratm1Mak mutation (1 available); any Il27ra mutation (17 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• in Con A-treated mice
• in vitro, mutant splenocytes show a slight increase in proliferation in response to stimulation with increasing concentrations of anti-CD3 or ConA
• purified mutant splenocytes show a relative increase in the number of cells in the S and G2+M phases of the cell cycle upon stimulation with anti-CD3 plus anti-CD28
• mutant splenocytes show normal dose-response kinetics in response to stimulation with anti-CD3 plus IL-12, but exhibit a higher proliferation rate than wild-type splenocytes at all but the highest dose of IL-12 upon co-stimulation with anti-CD28

mortality/aging

immune system
• in vitro, mutant splenocytes show a slight increase in proliferation in response to stimulation with increasing concentrations of anti-CD3 or ConA
• purified mutant splenocytes show a relative increase in the number of cells in the S and G2+M phases of the cell cycle upon stimulation with anti-CD3 plus anti-CD28
• mutant splenocytes show normal dose-response kinetics in response to stimulation with anti-CD3 plus IL-12, but exhibit a higher proliferation rate than wild-type splenocytes at all but the highest dose of IL-12 upon co-stimulation with anti-CD28
• homozygotes display only an initial impairment of IFN-gamma production and induction of Th1 responses during the early stages of infection with an intracellular pathogen (J:89509)
• in a model of experimental autoimmune uveoretinitis, mice exhibit lower Th1 response compared with wild-type mice (J:116610)
• in the early phase of experimental autoimmune uveoretinitis
• in vitro, isolated mutant T cells produce decreased IFN-gamma levels upon primary stimulation with IL-12 plus ConA or anti-CD3 (J:89509)
• however, surprisingly, fully differentiated mutant Th1 cells subjected to a secondary stimulation with ConA produce wild-type levels of IFN-gamma (J:89509)
• in stimulated CD4+ T cells (J:116610)
• in Con A-stimulated liver mononuclear cells
• in Con A-stimulated liver mononuclear cells
• in the early phase, with lower Th1 response
• sub-retinal transplants fail to transfer experimental autoimmune uveoretinitis
• following infection with Myobacterium bovis bacillus Calmette-Guerin (BCG), homozygotes exhibit 8 times more liver granulomas than control littermates at 2 weeks post-infection
• although mutant granulomas are abnormally large and poorly differentiated, no differences in liver CFU number or liver damage are observed relative to similarly-infected wild-type mice
• although mutant splenocytes produce less IFN-gamma in response to anti-CD3 plus IL-12 than wild-type cells at day 2 post-infection, IFN-gamma production is restored to normal levels at at day 7, and serum IFN-gamma levels are comparable to wild-type levels at 2 weeks post-infection
• homozygotes exhibit increased susceptibility to Leishmania major infection, showing increased footpad swelling, more severe footpad ulcerations, and increased parasite burdern relative to control littermates
• infected homozygotes exhibit an abnormal initial Th1 response, as shown by impaired IFN-gamma production by popliteal lymph node CD4+ lymphocytes during the early phases of Leishmania major infection (at day 5 and 2 weeks post-infection), but not during the later phases (at 4 and 6 weeks post-infection) when both IFN-gamma expression and production levels are restored to wild-type levels
• in contrast, IL-4 expression is normal at day 5 but elevated at 2 weeks post-infection or later, concomittant with higher serum levels of IgG1 and IgE at 9 weeks post-infection, indicating a deviation toward a Th2 cytokine profile

liver/biliary system
• in Con A-treated mice

homeostasis/metabolism
• in the early phase of experimental autoimmune uveoretinitis
• at a low dose of Con A
• however, treatment with anti-IFN-gamma or anti-IL4 antibodies or depletion of NK cells lowers ALT levels
• mice exhibit increased sensitivity to Con A-induced liver injury with increased mortality and increased cytokine serum levels compared with wild-type mice
• mice treated with a low dose of Con A exhibit increased alanine transaminase serum levels and massive liver necrosis compared with similarly treated wild-type mice

hematopoietic system
N
• homozygotes display normal hematopoietic and lymphoid development relative to wild-type and heterozygous control mice
• in vitro, mutant splenocytes show a slight increase in proliferation in response to stimulation with increasing concentrations of anti-CD3 or ConA
• purified mutant splenocytes show a relative increase in the number of cells in the S and G2+M phases of the cell cycle upon stimulation with anti-CD3 plus anti-CD28
• mutant splenocytes show normal dose-response kinetics in response to stimulation with anti-CD3 plus IL-12, but exhibit a higher proliferation rate than wild-type splenocytes at all but the highest dose of IL-12 upon co-stimulation with anti-CD28
• homozygotes display only an initial impairment of IFN-gamma production and induction of Th1 responses during the early stages of infection with an intracellular pathogen (J:89509)
• in a model of experimental autoimmune uveoretinitis, mice exhibit lower Th1 response compared with wild-type mice (J:116610)




Genotype
MGI:4943708
hm2
Allelic
Composition
Il27ratm1Mak/Il27ratm1Mak
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il27ratm1Mak mutation (1 available); any Il27ra mutation (17 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• in an E. coli-induced arthritis model, mice exhibit longer sustained inflammation and greater bone erosion compared with wild-type mice

skeleton
• in an E. coli-induced arthritis model, mice exhibit longer sustained inflammation and greater bone erosion compared with wild-type mice




Genotype
MGI:4943707
cx3
Allelic
Composition
Il10tm1Cgn/Il10tm1Cgn
Il27ratm1Mak/Il27ratm1Mak
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il10tm1Cgn mutation (15 available); any Il10 mutation (38 available)
Il27ratm1Mak mutation (1 available); any Il27ra mutation (17 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• compared with Il10tm1Cgn homozygotes

immune system
• helminth-induced colitis compared with similarly treated Il10tm1Cgn homozygotes
• delayed and milder onset compared with Il10tm1Cgn homozygotes
• mice exhibit lower Th1 response compared with Il10tm1Cgn homozygotes
• helminth-infected mice exhibit a decrease in Th1 response and an increase in Th2 response compared with similarly treated Il10tm1Cgn homozygotes
• helminth-infected mice exhibit a decrease in Th1 response and an increase in Th2 response compared with similarly treated Il10tm1Cgn homozygotes
• in stimulated lymphocytes
• in mesenteric lymph node cells from helminth-infected mice compared with similarly treated Il10tm1Cgn homozygotes
• in mesenteric lymph node cells from helminth-infected mice compared with similarly treated Il10tm1Cgn homozygotes

digestive/alimentary system
• helminth-induced colitis compared with similarly treated Il10tm1Cgn homozygotes
• delayed and milder onset compared with Il10tm1Cgn homozygotes

hematopoietic system
• mice exhibit lower Th1 response compared with Il10tm1Cgn homozygotes
• helminth-infected mice exhibit a decrease in Th1 response and an increase in Th2 response compared with similarly treated Il10tm1Cgn homozygotes
• helminth-infected mice exhibit a decrease in Th1 response and an increase in Th2 response compared with similarly treated Il10tm1Cgn homozygotes




Genotype
MGI:4943704
cx4
Allelic
Composition
Faslpr/Faslpr
Il27ratm1Mak/Il27ratm1Mak
Genetic
Background
MRL.Cg-Il27ratm1Mak Faslpr
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Faslpr mutation (39 available); any Fas mutation (76 available)
Il27ratm1Mak mutation (1 available); any Il27ra mutation (17 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• 10% of mice die by week 24 compared with 50% of Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice

immune system
N
• mice exhibit unaltered lymphocyte compartment compared with Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• mice exhibit increased IgG1 depositions in the kidneys compared with Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• IgG1 serum levels are increased compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• mice exhibit reduced IgG2a renal deposits and serum levels compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• Th2-type immune response is favored compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• 10-fold in stimulated CD4+ T cells compared with similarly treated Faslpr/Faslpr Il27ratm1Mak/Il27ra+ cells
• mice exhibit improved survival, creatinine serum level, blood urea nitrogen serum level, and renal injury compared with Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• mice develop membranous glomerulonephritis

renal/urinary system
• mice develop membranous glomerulonephritis

homeostasis/metabolism
• compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice

hematopoietic system
• compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• mice exhibit increased IgG1 depositions in the kidneys compared with Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• IgG1 serum levels are increased compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• mice exhibit reduced IgG2a renal deposits and serum levels compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice
• Th2-type immune response is favored compared to in Faslpr/Faslpr Il27ratm1Mak/Il27ra+ mice

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
membranous glomerulonephritis DOID:10976 J:122148





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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory