Phenotypes associated with this allele

• Allele Symbol: Fabp5^tm1Hota
• Allele Name: targeted mutation 1, Gokhan S Hotamisligil
• Allele ID: MGI:2450172
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fabp5^tm1Hota/Fabp5^tm1Hota	B6.Cg-Fabp5^tm1Hota	MGI:3815422
cx2	Fabp4^tm1Brsp/Fabp4^tm1Brsp Fabp5^tm1Hota/Fabp5^tm1Hota	B6.Cg-Fabp4^tm1Brsp Fabp5^tm1Hota	MGI:3815451
cx3	Apoe^tm1Unc/Apoe^tm1Unc Fabp4^tm1Brsp/Fabp4^tm1Brsp Fabp5^tm1Hota/Fabp5^tm1Hota	B6.Cg-Fabp4^tm1Brsp Fabp5^tm1Hota Apoe^tm1Unc	MGI:3815438
cx4	Fabp4^tm1Brsp/Fabp4^tm1Brsp Fabp5^tm1Hota/Fabp5^tm1Hota Lep^ob/Lep^ob	B6.Cg-Fabp4^tm1Brsp Fabp5^tm1Hota Lep^ob	MGI:3815448
cx5	Fabp5^tm1Hota/Fabp5^tm1Hota Lep^ob/Lep^ob	B6.Cg-Fabp5^tm1Hota Lep^ob	MGI:3815449
cx6	Fabp5^tm1Hota/Fabp5^tm1Hota Lep^ob/Lep^ob	involves: 129 * C57BL/6J * C57BL/10J * SJL	MGI:3815423
cx7	Fabp4^tm1Brsp/Fabp4^tm1Brsp Fabp5^tm1Hota/Fabp5^tm1Hota	involves: 129S7/SvEvBrd * C57BL/6J * SJL	MGI:6113244

Genotype
MGI:3815422

hm1

• Allelic Composition: Fabp5^tm1Hota/Fabp5^tm1Hota
• Genetic Background: B6.Cg-Fabp5^tm1Hota

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:81609 )

• when fed a high fat diet mice weigh 10% less than wild type mice

decreased circulating glucose level ( J:81609 )

• compared to wild-type mice when fed a high fat diet

decreased circulating leptin level ( J:81609 )

• compared to wild-type mice when fed a standard or high fat diet

improved glucose tolerance ( J:81609 )

• compared to wild-type mice when fed a high fat diet

increased insulin sensitivity ( J:81609 )

• compared to wild-type mice when fed a high fat diet

adipose tissue

decreased subcutaneous adipose tissue amount ( J:81609 )

• compared to wild-type mice when fed a high fat diet

increased adipocyte glucose uptake ( J:81609 )

• un-stimulated and insulin stimulated adipocytes exhibit a 2-fold higher glucose uptake compared to wild-type adipocytes

liver/biliary system

decreased liver weight ( J:81609 )

• compared to wild-type mice when fed a high fat diet

growth/size/body

decreased susceptibility to diet-induced obesity ( J:81609 )

• when fed a high fat diet mice weigh 10% less than wild type mice

integument

decreased subcutaneous adipose tissue amount ( J:81609 )

• compared to wild-type mice when fed a high fat diet

cellular

increased adipocyte glucose uptake ( J:81609 )

• un-stimulated and insulin stimulated adipocytes exhibit a 2-fold higher glucose uptake compared to wild-type adipocytes

Genotype
MGI:3815451

cx2

• Allelic Composition: Fabp4^tm1Brsp/Fabp4^tm1Brsp; Fabp5^tm1Hota/Fabp5^tm1Hota
• Genetic Background: B6.Cg-Fabp4^tm1Brsp Fabp5^tm1Hota

homeostasis/metabolism

decreased circulating glucose level ( J:111878 )

• when fed a high fat diet

increased insulin sensitivity ( J:111878 )

• when fed a high fat diet, insulin resistance is partially recovered compared to similarly treated wild-type mice

• increased insulin sensitivity is greater when fed a high fat diet than in Fabp4^tm1Brsp Fabp5^tm1Hota Lep^ob homozygotes

Genotype
MGI:3815438

cx3

• Allelic Composition: Apoe^tm1Unc/Apoe^tm1Unc; Fabp4^tm1Brsp/Fabp4^tm1Brsp; Fabp5^tm1Hota/Fabp5^tm1Hota
• Genetic Background: B6.Cg-Fabp4^tm1Brsp Fabp5^tm1Hota Apoe^tm1Unc

mortality/aging

mortality/aging ( J:102223 )

N • mice fed a high fat diet exhibit a 100% survival rate at 1 year compared to a 33% survival for Apoe^tm1Unc homozygotes

homeostasis/metabolism

decreased circulating glucose level ( J:102223 )

• fasting serum glucose levels are reduced 48% in female mice compared to Apoe^tm1Unc homozygotes

• however, serum glucose levels in male mice is the same as in controls

decreased circulating cholesterol level ( J:102223 )

• fasting serum cholesterol levels are 18% lower in male mice and 29% lower in female mice compared to in Apoe^tm1Unc homozygotes

• however, serum cholesterol levels in female mice are the same as in controls

decreased circulating triglyceride level ( J:102223 )

• fasting serum triglyceride levels are 32% lower in male mice and 42% lower in female mice compared to in Apoe^tm1Unc homozygotes

• however, serum triglyceride levels in female mice are the same as in controls

improved glucose tolerance ( J:102223 )

• compared to Apoe^tm1Unc homozygotes

increased insulin sensitivity ( J:102223 )

• compared to Apoe^tm1Unc homozygotes

cardiovascular system

decreased susceptibility to atherosclerosis ( J:102223 )

• male mice exhibit a 53% reduction in mean atherosclerotic lesion and a 45% reduction in mean lesion are of the en face aorta compared to Apoe^tm1Unc homozygotes

• female mice exhibit a 37% reduction in mean atherosclerotic lesion and a 37% reduction in mean lesion are of the en face aorta compared to Apoe^tm1Unc homozygotes

• when fed a high fat diet, male mice exhibit a 26% reduction in atherosclerosis of the proximal aorta with a 78% reduction in the en face aorta lesion compared to in Apoe^tm1Unc homozygotes

growth/size/body

decreased body weight ( J:102223 )

• body weight of male mice is 10% lower than in Apoe^tm1Unc homozygotes

• however, female mice weigh the same as controls

Genotype
MGI:3815448

cx4

• Allelic Composition: Fabp4^tm1Brsp/Fabp4^tm1Brsp; Fabp5^tm1Hota/Fabp5^tm1Hota; Lep^ob/Lep^ob
• Genetic Background: B6.Cg-Fabp4^tm1Brsp Fabp5^tm1Hota Lep^ob

homeostasis/metabolism

decreased circulating cholesterol level ( J:111878 )

• compared to Lep^ob homozygotes

decreased circulating triglyceride level ( J:111878 )

• compared to Lep^ob homozygotes

abnormal glucose homeostasis ( J:111878 )

• insulin stimulated whole-body glucose turn-over is greater than in Lep^ob homozygotes

• insulin stimulated glycosis in skeletal muscle is increased compared to in Lep^ob homozygotes

• however, insulin stimulated glucose production is the same as in controls

decreased circulating glucose level ( J:111878 )

• in hyperinsulinemic-euglycemic clam studies, mice exhibit a basal glucose level that is lower than in Lep^ob homozygotes

• however, insulin stimulated glucose levels are the same as in controls and mice are euglycemic compared to wild-type mice

decreased circulating insulin level ( J:111878 )

• compared to Lep^ob homozygotes

abnormal gluconeogenesis ( J:111878 )

• in hyperinsulinemic-euglycemic clam studies, basal hepatic glucose production is 33% less than in Lep^ob homozygotes

improved glucose tolerance ( J:111878 )

• compared to Lep^ob homozygotes

abnormal glycogen homeostasis ( J:111878 )

• insulin stimulated glucogen synthesis in skeletal muscle is increased 3-fold compared to in Lep^ob homozygotes

increased insulin sensitivity ( J:111878 )

• compared to Lep^ob homozygotes

increased fatty acids level ( J:111878 )

• compared to Lep^ob homozygotes

decreased liver triglyceride level ( J:111878 )

• liver triglyceride levels are 27% less than in Lep^ob homozygotes

adipose tissue

increased white adipose tissue amount ( J:111878 )

• at 20 weeks of age, mice exhibit an increase in epididymal and subcutaneous adipose tissue compared to Lep^ob homozygotes

increased adipocyte glucose uptake ( J:111878 )

• un-stimulated and insulin stimulated adipocyte glucose uptake is higher than in Lep^ob homozygotes

muscle

increased muscle cell glucose uptake ( J:111878 )

• 2.5-fold higher than in Lep^ob homozygotes

liver/biliary system

decreased liver triglyceride level ( J:111878 )

• liver triglyceride levels are 27% less than in Lep^ob homozygotes

small liver ( J:111878 )

• compared to Lep^ob homozygotes

decreased susceptibility to hepatic steatosis ( J:111878 )

• compared to Lep^ob homozygotes

growth/size/body

increased body weight ( J:111878 )

• at 20 weeks of age, mice weigh more than Lep^ob homozygotes

cellular

increased adipocyte glucose uptake ( J:111878 )

• un-stimulated and insulin stimulated adipocyte glucose uptake is higher than in Lep^ob homozygotes

increased muscle cell glucose uptake ( J:111878 )

• 2.5-fold higher than in Lep^ob homozygotes

Genotype
MGI:3815449

cx5

• Allelic Composition: Fabp5^tm1Hota/Fabp5^tm1Hota; Lep^ob/Lep^ob
• Genetic Background: B6.Cg-Fabp5^tm1Hota Lep^ob

homeostasis/metabolism

improved glucose tolerance ( J:111878 )

• mice exhibit improved glucose tolerance compared to Lep^ob homozygotes but not as much as in Fabp4^tm1Brsp Fabp5^tm1Hota Lep^ob homozygotes

increased insulin sensitivity ( J:111878 )

• compared to Lep^ob homozygotes

Genotype
MGI:3815423

cx6

• Allelic Composition: Fabp5^tm1Hota/Fabp5^tm1Hota; Lep^ob/Lep^ob
• Genetic Background: involves: 129 * C57BL/6J * C57BL/10J * SJL

homeostasis/metabolism

decreased circulating glucose level ( J:81609 )

• compared to Lep^ob homozygotes

increased circulating insulin level ( J:81609 )

• similar to Lep^ob homozygotes, mice exhibit increased insulin levels compared to wild-type mice

improved glucose tolerance ( J:81609 )

• compared to Lep^ob homozygotes

increased insulin sensitivity ( J:81609 )

• compared to Lep^ob homozygotes

growth/size/body

obese ( J:81609 )

• similar to Lep^ob homozygotes, mice develop early-onset severe obesity comapred to wild-type mice

Genotype
MGI:6113244

cx7

• Allelic Composition: Fabp4^tm1Brsp/Fabp4^tm1Brsp; Fabp5^tm1Hota/Fabp5^tm1Hota
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J * SJL

adipose tissue

abnormal brown adipose tissue morphology ( J:217245 )

• in fasted mice, brown adipose tissue is darker with small multilocular lipid droplets

decreased brown adipose tissue mass ( J:217245 )

• in fasted, but not fed, mice at room temperature

• however, cold exposure does not exacerbate the phenotype

abnormal brown adipose tissue thermogenesis ( J:217245 )

• failure to increase glucose uptake in fed or fasted mice exposed to cold

• reduced fatty acid uptake in fed mice at room temperature and further decreased following cold-exposure

• however, fasted mice exhibit a normal decrease in fatty acid uptake

homeostasis/metabolism

impaired adaptive thermogenesis ( J:217245 )

• mice exposed to 4 degrees after a period of fasting exhibit a larger drop in body temperature than wild-type mice

• however, mice continuously fed and cold exposed exhibit normal surface body temperature

decreased circulating glucose level ( J:217245 )

• in fed or fasted mice after cold exposure

• however, cold exposure does not exacerbate hypoglycemia

increased circulating free fatty acids level ( J:217245 )

• non-esterified fatty acids in fed mice after cold exposure

decreased body surface temperature ( J:217245 )

• mice exposed to 4 degrees after a period of fasting exhibit a larger drop in body temperature than wild-type mice

• however, mice continuously fed and cold exposed exhibit normal surface body temperature

decreased glycogen level ( J:217245 )

• marginally reduced in fed mice after cold exposure

decreased skeletal muscle glycogen level ( J:217245 )

• in fed mice before cold exposure

• however, cold-exposure does not exacerbate the phenotype

decreased triglyceride level ( J:217245 )

• in cold-exposed, fasted mice

• however, fed mice exhibit normal levels even when cold-exposed

muscle

muscle phenotype ( J:217245 )

N • skeletal muscle exhibit normal glucose and fatty acid uptake whether fed or fasted and housed at room temperature or cold exposed

decreased skeletal muscle glycogen level ( J:217245 )

• in fed mice before cold exposure

• however, cold-exposure does not exacerbate the phenotype