Analysis Tools
mortality/aging
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• death between E4.5 and E7.5
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embryo
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• lacked normal embryonic architecture
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Allele Symbol Allele Name Allele ID |
Mdm2tm1Bay targeted mutation 1, Baylor College of Medicine MGI:1857463 |
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| Summary |
11 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• death between E4.5 and E7.5
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• lacked normal embryonic architecture
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 100% of mice die by day 14 after 10 Gy irradiation
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• 100% of mice die by day 14 after 10 Gy irradiation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• number of offspring is less than the expected 25%; percentage of female offspring is 15-16%
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• at 5 weeks of age, a 15-20% reduction in body weight is observed relative to wild-type mice
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• thymus wet weight is significantly less than in wild-type; weight is decreased 2.5-fold
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• decreased number of lymphoid cells; thymi are hypocellular
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• 87% reduction in number of more mature B220+ IgM+ B cells
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• number of lymphoid cells is decreased 7-fold; mutants have only ~50% as many bone marrow cells as wild-type mice at 5-6 weeks
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• red blood cell count is 82% of wild-type; 2-fold decrease in number of erythrocytes is found at 5-6 weeks
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• WBC count is only 30% of wild-type
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• 1.2-fold decrease
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• concentration of neutrophils is 60% of wild-type value
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• lymphocyte concentration is reduced to 37% of wild-type
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• 40% reduction in CD43+ B220+ immature B cells in bone marrow relative to wild-type
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• reduced to 40% of wild-type number
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• thymocyte number is 5% of wild-type
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• number of mature, double-negative thymocytes is reduced by >80% compared to wild-type
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• double positive thymocyte numbers are reduced in relative proportion from 40% in wild-type to 20% in mutants at 5-6 weeks
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• spleen wet weight is significantly less than in wild-type
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• decreased number of lymphoid cells
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• thymus wet weight is significantly less than in wild-type; weight is decreased 2.5-fold
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• decreased number of lymphoid cells; thymi are hypocellular
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• 87% reduction in number of more mature B220+ IgM+ B cells
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• WBC count is only 30% of wild-type
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• 1.2-fold decrease
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• concentration of neutrophils is 60% of wild-type value
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• lymphocyte concentration is reduced to 37% of wild-type
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• 40% reduction in CD43+ B220+ immature B cells in bone marrow relative to wild-type
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• reduced to 40% of wild-type number
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• thymocyte number is 5% of wild-type
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• number of mature, double-negative thymocytes is reduced by >80% compared to wild-type
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• double positive thymocyte numbers are reduced in relative proportion from 40% in wild-type to 20% in mutants at 5-6 weeks
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• spleen wet weight is significantly less than in wild-type
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• decreased number of lymphoid cells
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• liver wet weight is less than in wild-type
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• kidney wet weight is less than in wild-type
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• an increase in G1 arrest function is seen, with number of S-phase cells 72% of wild-type MEFs
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• exposure to 10 Gy of radiation results in 100% mortality within 7-9 days of treatment, compared to ~50% mortality in wild-type mice between 12 and 22 days after treatment
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• MEFs in culture show decreased rate of proliferation compared to wild-type or Mdm2-heterozygous cells
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• thymus wet weight is significantly less than in wild-type; weight is decreased 2.5-fold
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• decreased number of lymphoid cells; thymi are hypocellular
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• thymocyte number is 5% of wild-type
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no offspring heterozygous for the two mutant Mdm2 alleles are produced
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• female offspring are born at the expected frequency
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• thymus is same size as in wild-type mice; thymocytes show wild-type levels of apoptosis and B cells show wild-type levels also
• number of B220+ B cells in bone marrow is similar to wild-type mice
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• 1.9-fold increase in number of apoptotice B cells relative to wild-type bone marrow
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• 17-fold increase in spontaneous apoptosis, occurring mainly in the crypts rather than villi
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• 100% of animals survive at least 35 days after treatment
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• 100% of animals survive at least 35 days after treatment
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice are normal, fertile and indistinguishable from controls, suggesting that the absence of Trp53 resuces the embryonic lethal phenotype of the Mdm2tm1Bay homozygous mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• failed to rescue embryonic lethality due to the homozygous Mdm2tm1Bay condition
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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