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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Wt1tm1Jae
targeted mutation 1, Rudolf Jaenisch
MGI:1857268
Summary 9 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Wt1tm1Jae/Wt1tm1Jae involves: 129S4/SvJae MGI:5824270
hm2
Wt1tm1Jae/Wt1tm1Jae involves: 129S4/SvJae * BALB/c * C57BL/6 MGI:2669010
hm3
Wt1tm1Jae/Wt1tm1Jae involves: 129S4/SvJae * C3H * C57BL/6 MGI:2669011
hm4
Wt1tm1Jae/Wt1tm1Jae involves: 129S4/SvJae * C57BL/6 MGI:2665007
hm5
Wt1tm1Jae/Wt1tm1Jae involves: 129S4/SvJae * C57BL/6 * MF1 MGI:2669009
hm6
Wt1tm1Jae/Wt1tm1Jae involves: 129S4/SvJae * NMRI MGI:5508905
cn7
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Tbx18tm4(cre)Akis/Tbx18+
Wt1tm1Jae/Wt1tm1Jae
involves: 129S4/SvJae MGI:3841149
cn8
Plekha5Tg(AMH-cre)1Flor/0
Wt1tm1Jae/Wt1tm2Vih
involves: 129S4/SvJae * 129S7/SvEvBrd * C57BL/6 * SJL MGI:3690050
cx9
Pax21Neu/Pax2+
Wt1tm1Jae/Wt1+
involves: 102 * 129S4/SvJae * C57BL/6 MGI:3841045


Genotype
MGI:5824270
hm1
Allelic
Composition
Wt1tm1Jae/Wt1tm1Jae
Genetic
Background
involves: 129S4/SvJae
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Wt1tm1Jae mutation (3 available); any Wt1 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• complete lack of the posthepatic mesenchymal plate

muscle
• in some cases, persistent thoracic nephric ridges are seen in E12.5 and E13.5 embryos




Genotype
MGI:2669010
hm2
Allelic
Composition
Wt1tm1Jae/Wt1tm1Jae
Genetic
Background
involves: 129S4/SvJae * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Wt1tm1Jae mutation (3 available); any Wt1 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• homozygous offspring from intercrosses of heterozygous F1 mice survive to birth, but die immediately after due to impaired lung inflation

respiratory system




Genotype
MGI:2669011
hm3
Allelic
Composition
Wt1tm1Jae/Wt1tm1Jae
Genetic
Background
involves: 129S4/SvJae * C3H * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Wt1tm1Jae mutation (3 available); any Wt1 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• homozygous offspring from intercrosses of heterozygous F1 mice survive to birth, but die immediately after due to impaired lung inflation

respiratory system




Genotype
MGI:2665007
hm4
Allelic
Composition
Wt1tm1Jae/Wt1tm1Jae
Genetic
Background
involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Wt1tm1Jae mutation (3 available); any Wt1 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• death between E13 and E15; no homozygous embryos are recovered after E15.5

cardiovascular system
N
• normal aortic, pulmonary, mitral, and tricuspid valves
• normal atrial and ventricular septa
• small amount of pericardial blood observed in some mice

embryo
• degeneration of the metaneprhic blastema observed at E12
• apoptosis in 10 to 50% of the cells of the metanephric blastema
• retarded development of the gonadal ridge followed by arrest around E14

homeostasis/metabolism
• small amount of pericardial blood observed in some mice
• systemic edema

muscle
• incomplete development resulting in the herniation of lung tissue into the abdominal cavity

renal/urinary system
• arrested development
• degeneration of the metaneprhic blastema observed at E12
• apoptosis in 10 to 50% of the cells of the metanephric blastema

respiratory system
• impeded growth, putatively due to incomplete pleura expansion

cellular
• degeneration of the metaneprhic blastema observed at E12
• apoptosis in 10 to 50% of the cells of the metanephric blastema




Genotype
MGI:2669009
hm5
Allelic
Composition
Wt1tm1Jae/Wt1tm1Jae
Genetic
Background
involves: 129S4/SvJae * C57BL/6 * MF1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Wt1tm1Jae mutation (3 available); any Wt1 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• approximately 16% of homozygous offsrping from intercrosses of heterozygous F1 mice survive to birth, but died immediately after due to impaired lung inflation
• some embryos die in utero

muscle
• abnormal primordial diaphragm with the caudal and more anterior aspect unilaterally missing
• left-sided posterolateral defects
• however, some mice had no diaphragmatic hernias

respiratory system

endocrine/exocrine glands
• observed at E18.5 in mice on a background involving MF1

hematopoietic system
• increased apoptosis of primordial spleen cells
• spleen primordium is smaller at E13.5 and the connection to the prospective pancreas is shortened
• at E15.5, only some thickening of the mesogastrium is observed and not a well-developed spleen as in wild-type
• stromal cells fo the spleen fail to develop
• almost complete absence of the spleen, correlated with increased apoptosis of primordial spleen cells

immune system
• increased apoptosis of primordial spleen cells
• spleen primordium is smaller at E13.5 and the connection to the prospective pancreas is shortened
• at E15.5, only some thickening of the mesogastrium is observed and not a well-developed spleen as in wild-type
• stromal cells fo the spleen fail to develop
• almost complete absence of the spleen, correlated with increased apoptosis of primordial spleen cells

cellular
• increased apoptosis of primordial spleen cells

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
congenital diaphragmatic hernia DOID:3827 OMIM:142340
OMIM:222400
OMIM:610187
J:114565




Genotype
MGI:5508905
hm6
Allelic
Composition
Wt1tm1Jae/Wt1tm1Jae
Genetic
Background
involves: 129S4/SvJae * NMRI
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Wt1tm1Jae mutation (3 available); any Wt1 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
embryo
• partial unilateral absence of pleuropericardial membranes in the hilus region resulting in pleural and pericardial cavities that are not separated at E14.5
• pleuropericardial membrane development is disturbed in embryos such that sinuatrial mesenchymal ridges form but they fail to fuse with the lining of the dorsal body wall or the mesothelial lining of the lung hilus
• pericardioperitoneal canals are not closed at E14.5
• slight, but significant, increase in proliferation is seen in the sinuatrial ridges at E12.5




Genotype
MGI:3841149
cn7
Allelic
Composition
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Tbx18tm4(cre)Akis/Tbx18+
Wt1tm1Jae/Wt1tm1Jae
Genetic
Background
involves: 129S4/SvJae
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1Sor mutation (7 available); any Gt(ROSA)26Sor mutation (492 available)
Tbx18tm4(cre)Akis mutation (0 available); any Tbx18 mutation (17 available)
Wt1tm1Jae mutation (3 available); any Wt1 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• the formation of the epicardium is severely compromised in E12.5 embryos




Genotype
MGI:3690050
cn8
Allelic
Composition
Plekha5Tg(AMH-cre)1Flor/0
Wt1tm1Jae/Wt1tm2Vih
Genetic
Background
involves: 129S4/SvJae * 129S7/SvEvBrd * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Plekha5Tg(AMH-cre)1Flor mutation (1 available); any Plekha5 mutation (27 available)
Wt1tm1Jae mutation (3 available); any Wt1 mutation (12 available)
Wt1tm2Vih mutation (1 available); any Wt1 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• in addition to reduced testes size, remainder of reproductive tract is hypoplastic
• histological analysis of proximal portion of male reproductive tract shows structures with coiled morphology and folded epithelium structure of oviduct
• at E13.5, male testicular development, including Sertoli cell location and numbers appears normal, but from E15.5 to P7, development is abnormal
• testes completely lack normal tubular architecture of controls and consist primarily of sheets of eosinophilic cells
• at E15.5, only a few tubule-like structures are observed
• testes contain sheets of eosinophilic cells identified histologically as Leydig cells; these cells are observed in dense clusters interspersed with regions of fibroblast-like cells
• in 7 week old males, testes size is ~10% of control wild-type littermates
• males have a uterus in addition to vas deferens, epididymis, and seminal vesicles
• histological analysis of proximal portion of male reproductive tract shows structures with coiled morphology and folded epithelium structure of oviduct
• based on aberrant structure of male testes, males are predicted to be sterile

endocrine/exocrine glands
• at E13.5, male testicular development, including Sertoli cell location and numbers appears normal, but from E15.5 to P7, development is abnormal
• testes completely lack normal tubular architecture of controls and consist primarily of sheets of eosinophilic cells
• at E15.5, only a few tubule-like structures are observed
• testes contain sheets of eosinophilic cells identified histologically as Leydig cells; these cells are observed in dense clusters interspersed with regions of fibroblast-like cells
• in 7 week old males, testes size is ~10% of control wild-type littermates




Genotype
MGI:3841045
cx9
Allelic
Composition
Pax21Neu/Pax2+
Wt1tm1Jae/Wt1+
Genetic
Background
involves: 102 * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax21Neu mutation (1 available); any Pax2 mutation (16 available)
Wt1tm1Jae mutation (3 available); any Wt1 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• fewer than expected mice are observed at E16
• 49% fewer than expected mice survive beyond weaning compared

renal/urinary system
• at E16, whole kidney apoptosis is increased compared to in wild-type mice
• the volume of collecting ducts is reduced compared to in wild-type mice
• compared to in Pax21Neu heterozygotes and wild-type mice
• at E16 and E19, mice exhibit abnormal kidney development that is more severe than in Pax21Neu heterozygotes
• however, kidney development at E13 is normal
• renal calyces are reduced in size and number compared to in wild-type mice
• the left kidney is 48% smaller than normal while the right kidney is 58% smaller than normal
• kidneys contain fewer than normal nephrons with fewer mesenchymal condensates, comma-shaped bodies, and S-shaped bodies and looser mesenchyme adjacent to ureteric bud structures compared to in wild-type kidneys
• in 20% of mice

cellular
• at E16, whole kidney apoptosis is increased compared to in wild-type mice





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last database update
05/14/2019
MGI 6.14
The Jackson Laboratory