Tbx18^tm4(cre)Akis
Targeted Allele Detail

Summary

• Symbol: Tbx18^tm4(cre)Akis
• Name: T-box18; targeted mutation 4, Andreas Kispert
• MGI ID: MGI:3841145
• Synonyms: Tbx18^Cre
• Gene: Tbx18 Location: Chr9:87584853-87613313 bp, - strand Genetic Position: Chr9, 47.06 cM, cytoband E3.2
• Alliance: Tbx18^tm4(cre)Akis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147421
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S1/SvImJ

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase)
• Mutation: Insertion
• Tbx18^tm4(cre)Akis expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tbx18 (MGI:1923615)

• Mutation details: A targeting vector was designed to insert a Cre ORF followed by a PGK-neo cassette flanked by FRT sites into the Tbx18 locus. After homologous recombination the ATG codon of Cre replaced the endogenous Tbx18 start codon. The vector was linearized and electroporated into 129S1/SvImJ ES cells. Clones that underwent homologous recombination correctly were identified by Southern blotting and injected into CD-1 morulae. Chimeric males were obtained and mated to a FLPe deleter line. When mice carrying this allele were crossed with mice carrying the cre reporter allele Gt(ROSA)26Sor^tm1Sor, cre expression was detected in numerous locations of E12.5 embyros including the epicardium, the interventricular septum and myocardial wall of the left ventricle. (J:147421, J:160473)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tbx18 (mouse) Summary of all recombinase alleles driven by Tbx18. Alliance mouse Tbx18 gene page

Expression

In Mice Carrying this Mutation:	316 assay results 9 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbx18 Mutation: 36 strains or lines available

References

• Original: J:147421 Christoffels VM, et al., Tbx18 and the fate of epicardial progenitors. Nature. 2009 Apr 16;458(7240):E8-9; discussion E9-10
• All: 26 reference(s)