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Targeted Allele Detail
Symbol: Apptm3.1Zhe
Name: amyloid beta (A4) precursor protein; targeted mutation 3.1, Hui Zheng
MGI ID: MGI:4847574
Synonyms: APP/hAbeta/mutC, APPki
Gene: App  Location: Chr16:84954440-85173707 bp, - strand  Genetic Position: Chr16, 46.92 cM, cytoband C3-qter
Amyloid beta deposits in Apptm3.1Zhe/Apptm3.1Zhe Psen1tm1Mpm/Psen1tm1Mpm mice

Show the 2 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:166379
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Humanized sequence, Inserted expressed sequence)
Mutations:    Insertion, Nucleotide substitutions
Apptm3.1Zhe expresses 1 gene
Mutation detailsExons 16 and 17 were replaced with a loxP site, a truncated exon 16 with a BACE cleavage site at K670M671 followed by a stop codon, an FRT-flanked neo cassette with a 3' loxP site, a modified exon 16 with the Swedish mutation K595N/M596L (APP695) and three additional mutations (G601R, F606Y, R609H) to humanize the sequence, and a modified exon 17 with the Arctic mutation E618G, London mutation V642I, silent mutation on G621S622 (GGTTCG to GGATCC) to create a BamHI site, and a frameshift mutation starting at I656H657H658 (ATC CAT CAT to ATC ATC AT) that results in a stop codon 30 bp after the frameshift side such that there are 10 resides behind I656. Cre-mediated recombination removed the truncated exon 16 and the neo cassette. (J:166379)
Generation of the Apptm3.1Zhe allele
View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any App Mutation:  20 strains or lines available
Original:  J:166379 Li H, et al., Genetic dissection of the amyloid precursor protein in developmental function and amyloid pathogenesis. J Biol Chem. 2010 Oct 1;285(40):30598-605
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
MGI 6.03
The Jackson Laboratory